The invention provides 'high-quality seafood
peptide' for preparing a natural
peptide-rich
flavor enhancer and a preparation method thereof. The preparation method mainly comprises the following steps of:
raw material pretreatment; controllable enzymolysis: mixing serum, adding water to regulate the serum till the concentration of the mixed serum is 30 to 50 weight percent, regulating the pH value to between 6.0 and 8.0, adding
papain to perform
hydrolysis, regulating the pH, heating the mixture, adding composite
flavor protease into the mixture, hydrolyzing the mixture, controlling the
solid concentration to be between 10 and 15 percent, and inactivating the
enzyme after the enzymolysis is finished to obtain a
hydrolysis product; centrifuging and
filtration;
fermentation and fishy smell removal: sterilizing the enzymolysis solution, then cooling the enzymolysis solution to
room temperature, inoculating
lactic acid bacteria according to the weight of the enzymolysis solution, and fermenting the solution to obtain
fermentation solution without fishy smell; ultra-
filtration: diluting the
fermentation solution till the
solid concentration is 2 to 5 percent, and obtaining a short
peptide-rich product with a molecular weight of below 6,000Da through an ultra-
filtration membrane with a
cut-off molecular weight of 6,000Da; concentration; high-pressure homogenization; and
spray drying. The prepared
flavor enhancer has double effects on flavor and
nutrition, and can be widely applied to the food field of condiments, meat products, quick-frozen products, instant noodles, expanding leisure food and the like.