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Method for enriching and purifying anthocyanin in lonicera caerulea

A technology of indigo honeysuckle and anthocyanins, applied in chemical instruments and methods, organic chemistry, preparation of sugar derivatives, etc., can solve the problems of low extraction purity and low extraction rate, and achieve high extraction purity, large adsorption capacity, and raw materials Source Rich Effects

Inactive Publication Date: 2012-07-04
DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings of conventional techniques such as relatively low extraction rate and low extraction purity, and provide a method for enriching and purifying anthocyanins in Lonicera japonica Lonicerae by using a macroporous adsorption resin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: A method for enriching and purifying anthocyanins in Lonicera edulis by using macroporous adsorption resin, the steps are as follows:

[0027] (1) The dried Lonicera edulis was crushed and passed through a 60-mesh sieve, and extracted with ethanol with 15 times the volume of Lonicera edulis powder at reflux for 3 times, each for 2 hours;

[0028] (2) Suction filtration of the Lonicera edulis extract processed in step (1), and recovering ethanol from the extract under reduced pressure to obtain an initial extract;

[0029] (3) Adjust the pH of the obtained primary extract with acid to 2.3, stand still, filter with suction, and recover the solvent under reduced pressure to obtain a crude extract of Lonicera edulis;

[0030] (4) Adsorb the obtained crude extract of Lonicera edulis Lonicera japonica on a macroporous adsorption resin chromatography column, the macroporous resin used is the NKA-II type resin produced by Tianjin Nankai University;

[0031] (5) Use 30%, 40%, 5...

Embodiment example 2

[0035] Implementation case 2: A method for enriching and purifying anthocyanins in Lonicera edulis by using macroporous adsorption resin, the steps are as follows:

[0036] (1) After crushing the dried Lonicera edulis Lonicera edulis through an 80-mesh sieve, reflux extraction with ethanol of 20 times the volume of Lonicera edulis Lonicera japonica powder for 3 times, each for 2 hours;

[0037] (2) Suction filtration of the Lonicera edulis extract processed in step (1), and recovering ethanol from the extract under reduced pressure to obtain an initial extract;

[0038] (3) Adjust the pH of the obtained initial extract with acid to 2.2, stand still, filter with suction, and recover the solvent under reduced pressure to obtain a crude extract of Lonicera edulis;

[0039] (4) Adsorb the obtained crude anthocyanin extract with a macroporous adsorption resin, the macroporous resin used is D4006 type resin produced by Tianjin Nankai University;

[0040] (5) Elute with 30%, 50%, 70%, 90% etha...

Embodiment example 3

[0044] Implementation case 3: A method for enriching and purifying anthocyanins in Lonicera edulis by using macroporous adsorption resin, the steps are as follows:

[0045] (1) After crushing the dried Lonicera edulis Lonicera edulis through a 40-mesh sieve, the volume of the Lonicera edulis Lonicera japonica powder is 20 times the volume of ethanol to extract 3 times, each for 2 hours;

[0046] (2) Suction filtration of the Lonicera edulis extract processed in step (1), and the extract is decompressed to recover ethanol to obtain the initial extract;

[0047] (3) Adjust the pH value of the obtained initial extract to 2.0 with acid, stand still, suction filter, and recover the solvent under reduced pressure to obtain a crude anthocyanin extract;

[0048] (4) Adsorb the obtained crude anthocyanin extract on a macroporous adsorption resin chromatography column. The macroporous resin used is the NKA-9 resin produced by Tianjin Nankai University;

[0049] (5) Use 40%, 60%, and 80% ethanol i...

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Abstract

The invention belongs to the field of natural organic chemistry, and relates to a method for enriching and purifying anthocyanin in lonicera caerulea by using macroporous adsorbent resin. The method is characterized in that: the anthocyanin is separated and purified by using a macroporous adsorption technology, the adsorptive selectivity to the anthocyanin is good, the adsorption speed and the resolution speed are high, and the adsorption capacity is high; the anthocyanin is extracted conveniently and fast, raw materials are rich in source, the production cost is low, the separation effect is obvious, the extraction purity is high, and an anthocyanin semifinished product containing over 35 percent of the anthocyanin and an anthocyanin finished product containing over 95 percent of the anthocyanin can be obtained; and the defects of low conventional extraction rate and low extraction purity are overcome. According to the method disclosed by the invention, the macroporous adsorbent resin which is stable in physicochemical property, large in surface area, high in exchange rate, high in mechanical strength, strong in anti-pollution capacity and high in thermal stability is used, so that the anthocyanin can be selectively adsorbed from a solution through physical adsorption, the adsorption speed and the resolution speed are high, and the adsorption capacity is high.

Description

Technical field: [0001] The invention belongs to the field of natural organic chemistry, and relates to a method for purifying anthocyanins, in particular to a method for enriching and purifying anthocyanins in Lonicera edulis by using a macroporous adsorption resin. Background technique: [0002] Lonicera edulis, also known as Lonicera edulis, goat's milk, black blinds, mountain eggplant, blue edulis, is a small perennial deciduous shrub of Loniceraceae Lonicera. The fruit is a berry, rich in sugars, organic acids, minerals, multiple vitamins and trace elements. It has high nutritional value and medicinal value, and is an ideal natural health nutritious food. The fruit can be eaten fresh and can be processed into beverages, suitable for extracting natural purple pigment. The seeds contain anthocyanin, and the whole plant leaves contain aucubin, which has certain medical value. Lonicera edulis contains anthocyanin, leuco anthocyanin, catechin and rutane. It has been clinically ...

Claims

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Application Information

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IPC IPC(8): C07H17/065C07H1/08
Inventor 姚德坤姚德利闫峰黄运生刘德文姚航郑君
Owner DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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