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Method for fast efficiently extracting and purifying anthocyanin from lonicera caerulea

A technology of cyanidinus and anthocyanins, which is applied in the field of fast and efficient extraction and purification of cyanidin-3-O-glucoside, can solve the problems of long extraction time, low yield, large investment, etc., and achieve efficient and reasonable utilization, and obtain The effect of high efficiency and high purity

Active Publication Date: 2017-11-07
黑龙江伊蓝生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional organic solvent extraction method has long extraction time and low yield; however, supercritical fluid extraction of anthocyanins has the disadvantages of large investment and high energy consumption.

Method used

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  • Method for fast efficiently extracting and purifying anthocyanin from lonicera caerulea
  • Method for fast efficiently extracting and purifying anthocyanin from lonicera caerulea
  • Method for fast efficiently extracting and purifying anthocyanin from lonicera caerulea

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example 1

[0023] A method for quickly and efficiently extracting and purifying anthocyanins from Cydola edulis is carried out according to the following process steps:

[0024] A. High-speed homogeneous crushing of enzymatically hydrolyzed indigo fruit: 1 kg of fresh blue indigo fruit and 5 L of water were subjected to high-speed homogeneous crushing for 6 minutes, the pH of the slurry was adjusted to 4.0 with citric acid, cellulase was added to make the enzyme concentration 450U / mL, and then Enzymolysis at 40°C for 3 hours, filtering, and collecting the filtrate and filter residue;

[0025] B. Negative-pressure cavitation-enhanced extraction of indigo fruit filter residue: add 15L ethanol solution to the above-mentioned filter residue according to the mass-volume ratio for negative-pressure cavitation-enhanced extraction. The extraction temperature is 40°C, the extraction pressure is -0.05MPa, and the extraction is performed twice, each time for 50min , Combine the extract and the filt...

example 2

[0030] A method for quickly and efficiently extracting and purifying anthocyanins from Cydola edulis is carried out according to the following process steps:

[0031] A. High-speed homogeneous crushing and enzymatic hydrolysis of blue indigo fruit raw materials: 1kg of fresh blue indigo fruit and 7L of water were subjected to high-speed homogeneous crushing for 8 minutes, the pH of the slurry was adjusted to 5.0 with glacial acetic acid, and the compound enzyme of cellulase and pectinase was added to make the cellulose Enzyme concentration is 300U / mL, pectinase concentration is 200U / mL, then enzymolysis at 45°C for 4h, filter, collect filtrate and filter residue;

[0032] B. Negative-pressure cavitation-enhanced extraction of indigo fruit filter residue: add 10L ethanol solution to the above-mentioned filter residue according to the mass-volume ratio for negative-pressure cavitation-enhanced extraction. The extraction temperature is 35°C, the extraction pressure is -0.07MPa, an...

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Abstract

The invention relates to a method for fast efficiently extracting and purifying cyanidin-3-O-glucoside from lonicera caerulea. The adopted technical scheme includes: taking a lonicera caerulea extract as a raw material, and adopting high-speed homogenized pulverized enzyme hydrolysis technology, negative-pressure cavitation reinforced extraction technology, low-temperature flocculent settling technology, medium-pressure quick-flash reverse column chromatographic technology and recrystallization technology to obtain refined cyanidin-3-O-glucoside having the purity more than 95%. The method is green and eco-friendly, less in steps, short in time consumption, less in loss of a target compound, high in product yield and purity, and suitable for massive production. The method has high economic value.

Description

technical field [0001] The invention relates to a method for extracting and purifying anthocyanin as a functional active ingredient from L. indigo fruit, in particular to a method for rapidly and efficiently extracting and purifying cyanidin-3-O-glucoside from L. indigo fruit. Background technique [0002] Lonicera caerulea L. is a small perennial deciduous shrub belonging to Lonicera caerulea. It has the effects of clearing heat and detoxifying, stabilizing blood pressure, improving liver detoxification function, anti-oxidation, anti-inflammation, anti-tumor, anti-fatigue, regulating immunity, protecting eyesight, and promoting good body development. The fruit of L. indigo is rich in anthocyanin compounds. Anthocyanin is a water-soluble natural pigment widely present in plants. It is a type of compound formed by combining anthocyanin and sugar with glycosidic bonds. The structural core is 3, 5,7-Trihydroxy-2-phenylbenzopyranium cation. It is safe, non-toxic, high in nutri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/065C07H1/08
CPCC07H1/08C07H17/065
Inventor 付玉杰焦骄李璐盖庆岩王微王鹏王希清
Owner 黑龙江伊蓝生物科技有限公司
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