Method for preparing animal globin polypeptide powder

Abstract

The application relates to the technical field of polypeptide preparation, and particularly discloses a preparation method of animal blood globin polypeptide powder. The preparation method of the animal blood globin polypeptide powder comprises the following steps: adding trisodium citrate into fresh animal blood, stirring for 10-30 min, adding cyanidin and polylysine, stirring uniformly, placing in a temperature-10 to-20 DEG C environment for 1-2 h, increasing to room temperature, homogenizing for 1-5 min, centrifuging, removing supernatant, and obtaining animal blood globin solution; adding the animal blood globin solution and rare earth lanthanum chitosan amine chelate salt into a glycine-sodium hydroxide buffer solution, stirring at 40-50 DEG C for 10-30 min, adding compound protease, and continuing to stir for 10-20 h to obtain an enzymatic solution; adjusting the pH value of the enzymatic solution to 5-6, increasing the temperature to 90-100 DEG C for 5-10 min, decreasing to room temperature, centrifuging for 5-10 min, and taking supernatant for low-temperature spray drying.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of polypeptide preparation, and particularly relates to a preparation method of animal blood globin polypeptide powder. BACKGROUND

[0002] Animal blood contains various nutritional components, such as protein, amino acid, vitamin, saccharide, lipid, mineral element and the like, wherein the fresh blood protein content is as high as 22%, which is the largest animal polypeptide powder resource. At present, only 20% of animal blood is reasonably utilized, and most of the animal blood is discharged as waste, thus causing serious pollution to the environment. Therefore, how to reasonably utilize the slaughter animal blood and improve the centralized utilization rate is crucial.

[0003] The potential nutritional value of animal blood is very high, but the utilization of animal blood is still very insufficient at present. At present, the chemical treatment method is mainly used for research, which not only pollutes the environment, but also results in poor stability and effect of the obtained polypeptide powder. With the development of enzyme preparation industry, the cost of enzyme preparation products is gradually reduced, which also promotes the utilization of enzymes in animal blood globin polypeptide powder. Due to the different types of enzymes, the conditions for enzymolysis of animal blood globin are different, and the enzymolysis effect is also different.

[0004] CN110904177A discloses a pig blood globin polypeptide powder and a preparation method and application thereof, which is prepared by the following steps: obtaining blood globin cells by pretreating pig blood; obtaining blood globin lysate by crushing and homogenizing the blood globin cells; obtaining enzyme lysate by enzyme treatment; obtaining product diluent after enzyme inactivation; obtaining product concentrate after concentration treatment; and obtaining the final product by sterile filtration treatment, freeze-drying, crushing and packaging the product concentrate. However, the product has poor stability, and the processing cost and production difficulty are large, which is not conducive to practical application.

[0005] Therefore, in view of the above problems, how to provide an animal blood globin polypeptide powder production process with good enzymolysis effect, good stability and excellent antioxidant activity has become a technical problem to be solved at present. SUMMARY

[0006] The present application aims at solving the problems in the prior art and provides a preparation method of animal blood globin polypeptide powder.

[0007] The present application aims at solving the problems in the prior art and provides a preparation method of animal blood globin polypeptide powder.

[0008] S1, tri-sodium citrate is added to fresh animal blood and stirred for 10-30 min, anthocyanins and polylysine are added and stirred uniformly, and then the mixture is placed in an environment with a temperature of-10 to-20℃ for 1-2 h, and then the temperature is increased to room temperature, and the mixture is subjected to homogenization treatment for 1-5 min, and then centrifuged to remove the supernatant, and thus animal blood globin liquid is obtained;

[0009] S2, adding animal blood globulin solution, rare earth lanthanum shell glucosamine chelate salt and glycine-sodium hydroxide buffer solution, stirring at 40-50℃ for 10-30min, adding compound protease and continuing to stir for 10-20h to obtain an enzymatic hydrolysate;

[0010] S3, adjusting the pH value of the enzymatic hydrolysate to 5-6, heating to 90-100℃ for 5-10min, cooling to room temperature, centrifuging for 5-10min, and taking the supernatant for low-temperature spray drying.

[0011] Preferably, in S1, the fresh animal blood is one of chicken blood, duck blood, pig blood, cow blood and sheep blood.

[0012] Preferably, in S1, the mass ratio of fresh animal blood, trisodium citrate, anthocyanin and polylysine is 100:1-3:1-2:0.1-1.

[0013] Preferably, in S1, the speed of heating to room temperature is 1-2℃ / min, and the homogenization speed is 8000-10000r / min.

[0014] Preferably, in S2, the mass ratio of animal blood globulin solution, rare earth lanthanum shell glucosamine chelate salt, glycine-sodium hydroxide buffer solution and compound protease is 30-50:1-2:200-300:0.1-1.

[0015] Preferably, in S2, the concentration of the glycine-sodium hydroxide buffer solution is 0.01-0.02mol / L, and the pH value thereof is 9-9.6.

[0016] Preferably, in S2, the specific activity of the compound protease is 5000-8000U / g.

[0017] Preferably, in S2, the compound protease comprises alkaline protease.

[0018] Preferably, in S2, the alkaline protease is at least one of bread yeast alkaline protease, serine alkaline protease, bacillus subtilis alkaline protease and streptomycin alkaline protease.

[0019] Preferably, in S3, the pH value of the enzymatic hydrolysate is adjusted to 5-6 by using 1-2mol / L hydrochloric acid.

[0020] Preferably, in S3, the centrifugation speed is 5000-8000r / min.

[0021] Preferably, in S3, the low-temperature spray drying temperature is 40-50℃.

[0022] An animal blood globulin polypeptide powder is prepared by the above-mentioned method.

[0023] Beneficial effects:

[0024] The fresh animal blood is subjected to freezing treatment to cause a large number of blood cell lysis and fragmentation, and then subjected to homogenization treatment, and the fragmentation effect is excellent; anthocyanins and polylysine are compounded to effectively inhibit the oxidation and denaturation problems in the freezing treatment, and to inhibit the damage of ice crystals to proteins, so that the protein content is increased, and the antioxidant activity of polypeptides is effectively ensured.

[0025] The animal blood cell liquid is compounded with rare earth lanthanum shell sugar amine chelate salt, and subjected to complex protease enzymolysis to form a peptide segment with a small molecular weight, the polypeptide has uniform molecular weight distribution, good solubility, and excellent antioxidant activity. It is found through experiments that the animal blood cell polypeptide powder obtained by the application has a strong DPPH free radical scavenging capacity, which can reach 97.9%.

[0026] Meanwhile, the animal blood cell polypeptide powder obtained by the application can also improve intestinal mucosal immunity and protect intestinal health, has high safety, no side effects, no bad flavor, and is suitable for large-scale industrial production. BRIEF DESCRIPTION OF DRAWINGS

[0027] Figure 1 A comparison chart of the degree of hydrolysis and the crude protein content of the animal blood cell polypeptide powder obtained in Example 5 and Comparative Examples 1-3.

[0028] Figure 2 A comparison chart of the percentage of peptide molecular weight in the animal blood cell polypeptide powder obtained in Example 5 and Comparative Examples 1-3.

[0029] Figure 3 A comparison chart of the color of the animal blood cell polypeptide powder obtained in Example 5 and Comparative Examples 1-3.

[0030] Figure 4 A comparison chart of the DPPH free radical scavenging capacity and superoxide anion scavenging capacity of the animal blood cell polypeptide powder obtained in Example 5 and Comparative Examples 1-3.

[0031] Figure 5 A comparison chart of the reducing power and metal ion chelating capacity of the animal blood cell polypeptide powder obtained in Example 5 and Comparative Examples 1-3. DETAILED DESCRIPTION

[0032] The application will be further described below in combination with specific embodiments.

[0033] Example 1

[0034] A method for preparing animal blood cell polypeptide powder, comprising the following steps:

[0035] S1, 10 g of trisodium citrate is added to 1000 g of chicken blood, stirred at a speed of 3000 r / min for 10 min, 10 g of anthocyanins and 1 g of polylysine are stirred uniformly, placed in a temperature of-10℃ environment for 1 h, increased to room temperature at a speed of 1℃ / min, homogenized for 1 min, the homogenization speed is 8000 r / min, centrifuged, the supernatant is removed, and animal blood globin solution is obtained;

[0036] S2, 300 g of animal blood globin solution and 10 g of rare earth lanthanum chitosan amine chelate salt are added into 2000 g of glycine-sodium hydroxide buffer solution with pH=9 and a concentration of 0.01 mol / L, stirred at a temperature of 40℃ for 10 min, the stirring speed is 50 r / min, 1 g of compound protease with specific activity of 5000 U / g (composed of papain and bacillus subtilis alkaline protease) is added, and the stirring is continued for 10 h, and an enzymatic hydrolysate is obtained;

[0037] S3, the pH value of the enzymatic hydrolysate is adjusted to 5 by using hydrochloric acid with a concentration of 1 mol / L, the temperature is increased to 90℃ and kept for 5 min, the temperature is decreased to room temperature, centrifuged at a speed of 5000 r / min for 5 min, and the supernatant is taken and spray dried at a low temperature of 40℃.

[0038] Example 2

[0039] A preparation method of animal blood globin polypeptide powder, comprising the following steps:

[0040] S1, 30 g of trisodium citrate is added to 1000 g of sheep blood, stirred at a speed of 4000 r / min for 30 min, 20 g of anthocyanins and 10 g of polylysine are stirred uniformly, placed in a temperature of-20℃ environment for 2 h, increased to room temperature at a speed of 2℃ / min, homogenized for 5 min, the homogenization speed is 10000 r / min, centrifuged, the supernatant is removed, and animal blood globin solution is obtained;

[0041] S2, 500 g of animal blood globin solution and 20 g of rare earth lanthanum chitosan amine chelate salt are added into 3000 g of glycine-sodium hydroxide buffer solution with pH=9.6 and a concentration of 0.02 mol / L, stirred at a temperature of 50℃ for 30 min, the stirring speed is 100 r / min, 10 g of compound protease with specific activity of 8000 U / g (composed of papain and streptomycin alkaline protease) is added, and the stirring is continued for 20 h, and an enzymatic hydrolysate is obtained;

[0042] S3, the pH value of the enzymatic hydrolysate is adjusted to 6 by using hydrochloric acid with a concentration of 2 mol / L, the temperature is increased to 100℃ and kept for 10 min, the temperature is decreased to room temperature, centrifuged at a speed of 8000 r / min for 10 min, the supernatant is taken, and spray dried at a low temperature of 50℃.

[0043] Example 3

[0044] A method for preparing animal blood cell polypeptide powder, comprising the following steps:

[0045] S1, 15g of trisodium citrate is added to 1000g of pig blood, stirred at a speed of 3700r / min for 15min, 17g of anthocyanins and 2g of polylysine are added and stirred uniformly, placed in a temperature-17℃ environment for 80min, increased to room temperature at a speed of 1.8℃ / min, homogenized for 2min at a homogenization speed of 9500r / min, centrifuged, and the supernatant is removed to obtain animal blood cell liquid;

[0046] S2, 350g of animal blood cell liquid and 17g of rare earth lanthanum chitosan amine chelate salt are added to 2200g of glycine-sodium hydroxide buffer solution with pH=9.3 and a concentration of 0.012mol / L, stirred at a temperature of 48℃ for 15min at a stirring speed of 90r / min, 3g of compound protease with specific activity of 7000U / g (consisting of streptomycin alkaline protease and serine alkaline protease) is added, and stirring is continued for 13h to obtain an enzymatic hydrolysate;

[0047] S3, the pH value of the enzymatic hydrolysate is adjusted to 5.5 by using 1.8mol / L hydrochloric acid, heated to 92℃ for 9min, cooled to room temperature, centrifuged at a speed of 6000r / min for 9min, and the supernatant is taken and spray dried at a low temperature of 42℃.

[0048] Example 4

[0049] A method for preparing animal blood cell polypeptide powder, comprising the following steps:

[0050] S1, 25g of trisodium citrate is added to 1000g of pig blood, stirred at a speed of 3300r / min for 25min, 13g of anthocyanins and 8g of polylysine are added and stirred uniformly, placed in a temperature-13℃ environment for 100min, increased to room temperature at a speed of 1.2℃ / min, homogenized for 4min at a homogenization speed of 8500r / min, centrifuged, and the supernatant is removed to obtain animal blood cell liquid;

[0051] S2, 450g of animal blood cell liquid and 13g of rare earth lanthanum chitosan amine chelate salt are added to 2800g of glycine-sodium hydroxide buffer solution with pH=9.3 and a concentration of 0.018mol / L, stirred at a temperature of 42℃ for 25min at a stirring speed of 70r / min, 7g of compound protease with specific activity of 6000U / g (consisting of bread yeast alkaline protease and serine alkaline protease) is added, and stirring is continued for 17h to obtain an enzymatic hydrolysate;

[0052] S3, the pH value of the enzymatic hydrolysate is adjusted to 5.5 by using 1.2 mol / L hydrochloric acid, the temperature is raised to 98℃ for 7 min, and then the temperature is reduced to room temperature, and the supernatant is obtained by centrifugation at 7000 r / min for 7 min, and then the supernatant is spray dried at 48℃.

[0053] Example 5

[0054] A method for preparing animal blood polypeptide powder, comprising the following steps:

[0055] S1, 20g of trisodium citrate is added to 1000g of pig blood, stirred at a speed of 3500r / min for 20min, 15g of anthocyanin and 5g of polylysine are added and stirred uniformly, placed in a temperature-15℃ environment for 90min, and then the temperature is raised to room temperature at a speed of 1.5℃ / min, homogenized for 3min at a homogenization speed of 9000r / min, centrifuged, and the supernatant is removed to obtain animal blood globin solution;

[0056] S2, 400g of animal blood globin solution and 15g of rare earth lanthanum chitosan amine chelate salt are added to 2400g of glycine-sodium hydroxide buffer solution with pH value of 9-9.6 and concentration of 0.015mol / L, stirred at a temperature of 45℃ for 20min at a stirring speed of 80r / min, 5g of compound protease with specific activity of 6500U / g (consisting of bread yeast alkaline protease and bacillus subtilis alkaline protease) is added, and the stirring is continued for 15h to obtain an enzymatic hydrolysate;

[0057] S3, the pH value of the enzymatic hydrolysate is adjusted to 5-6 by using 1.5 mol / L hydrochloric acid, the temperature is raised to 95℃ for 8 min, and then the temperature is reduced to room temperature, and the supernatant is obtained by centrifugation at 6500 r / min for 8 min, and then the supernatant is spray dried at 45℃.

[0058] Comparative Example 1

[0059] A method for preparing animal blood polypeptide powder, comprising the following steps:

[0060] S1, 20g of trisodium citrate is added to 1000g of pig blood, stirred at a speed of 3500r / min for 20min, 15g of anthocyanin is added and stirred uniformly, placed in a temperature-15℃ environment for 90min, and then the temperature is raised to room temperature at a speed of 1.5℃ / min, homogenized for 3min at a homogenization speed of 9000r / min, centrifuged, and the supernatant is removed to obtain animal blood globin solution;

[0061] S2, 400g of animal blood globulin solution, 15g of rare earth lanthanum chitosan chelate salt is added into 2400g of glycine-sodium hydroxide buffer solution with pH=9-9.6 and concentration of 0.015mol / L, stirring at 45℃ for 20min, stirring speed is 80r / min, 5g of compound protease with specific activity of 6500U / g (consisting of bread yeast alkaline protease and bacillus subtilis alkaline protease) is added, continue to stir for 15h, to obtain the enzymatic hydrolysate;

[0062] S3, the pH value of the enzymatic hydrolysate is adjusted to 5-6 by using 1.5mol / L hydrochloric acid, heated to 95℃ for 8min, reduced to room temperature, centrifuged at 6500r / min for 8min, take the supernatant, low temperature spray drying at 45℃.

[0063] Comparative example 2

[0064] A method for preparing animal blood globin polypeptide powder, comprising the following steps:

[0065] S1, 20g of trisodium citrate is added into 1000g of pig blood, stirring at 3500r / min for 20min, 15g of anthocyanin and 5g of polylysine are added and stirred uniformly, placed in a temperature of-15℃ environment for 90min, heated to room temperature at a speed of 1.5℃ / min, homogenized for 3min at a speed of 9000r / min, centrifuged, remove the supernatant, to obtain animal blood globulin solution;

[0066] S2, 400g of animal blood globulin solution is added into 2400g of glycine-sodium hydroxide buffer solution with pH=9-9.6 and concentration of 0.015mol / L, stirring at 45℃ for 20min, stirring speed is 80r / min, 5g of compound protease with specific activity of 6500U / g (consisting of bread yeast alkaline protease and bacillus subtilis alkaline protease) is added, continue to stir for 15h, to obtain the enzymatic hydrolysate;

[0067] S3, the pH value of the enzymatic hydrolysate is adjusted to 5-6 by using 1.5mol / L hydrochloric acid, heated to 95℃ for 8min, reduced to room temperature, centrifuged at 6500r / min for 8min, take the supernatant, low temperature spray drying at 45℃.

[0068] Comparative example 3

[0069] A method for preparing animal blood globin polypeptide powder, comprising the following steps:

[0070] S1. Add 20g of trisodium citrate to 1000g of pig blood, stir at 3500r / min for 20min, add 15g of anthocyanin and 5g of polylysine and stir evenly, place in an environment of -15℃ for 90min, raise to room temperature at a rate of 1.5℃ / min, homogenize for 3min at a homogenization speed of 9000r / min, centrifuge, remove the supernatant, and obtain animal blood cell fluid;

[0071] S2. Add 400g of animal blood cell solution and 15g of rare earth lanthanum chitosan chelate salt to 2400g of glycine-sodium hydroxide buffer solution with pH=9-9.6 and concentration of 0.015mol / L. Stir at 45℃ for 20min at a stirring speed of 80r / min. Add 5g of complex protease (composed of bromelain and trypsin) with a specific activity of 6500U / g. Continue stirring for 15h to obtain the enzymatic hydrolysate.

[0072] S3. Adjust the pH of the enzyme hydrolysate to 5-6 using 1.5 mol / L hydrochloric acid, heat to 95℃ and keep warm for 8 min, cool to room temperature, centrifuge at 6500 r / min for 8 min, take the supernatant and spray dry at 45℃.

[0073] The degree of hydrolysis (DH) of a protein refers to the percentage of peptide bonds that are broken during the protein hydrolysis reaction. This invention uses the o-phthalaldehyde (OPA) method to determine the degree of hydrolysis. Animal blood cell polypeptide powders obtained in Example 5 and Comparative Examples 1-3 were diluted with water by the same factor. 0.4 mL of each diluted solution was taken, and 3 mL of OPA reagent was added. The reaction was carried out precisely for 2 minutes, and the absorbance was measured at 340 nm.

[0074] The crude protein content of the animal blood cell polypeptide powders obtained in Example 5 and Comparative Examples 1-3 was determined using the Kjeldahl method.

[0075] like Figure 1 As shown, the animal blood cell polypeptide powder obtained in Example 5 had the highest degree of hydrolysis and crude protein content, which was superior to Comparative Examples 1-3 (P<0.05), confirming that the method of the present invention can effectively hydrolyze proteins in fresh animal blood.

[0076] The molecular weight distribution of the animal blood cell polypeptide powders obtained in Example 5 and Comparative Examples 1-3 was determined by high performance liquid chromatography. The injection volume of the prepared polypeptide samples and standards (molecular weights of 6500 Da, 13700 Da, and 43000 Da) was 100 μL, and the detection time was 50 min. The percentage of peptide molecular weight was calculated based on the peak value and peak area.

[0077] like Figure 2As shown, in the animal blood cell polypeptide powder obtained in Example 5, more than 60% had a molecular weight less than 1 kDa, which is superior to Comparative Examples 1-3 (P < 0.05). This confirms that the method of the present invention is more beneficial for the generation of small molecule peptides.

[0078] The colorimetry of the animal blood cell polypeptide powders obtained in Example 5 and Comparative Examples 1-3 was measured using a Minolta CR-410 colorimeter. L value represents black-to-white (brightness), with a higher value indicating greater whiteness (brightness); a value represents green-to-red, with a higher value indicating greater redness; b value represents blue-to-yellow, with a higher value indicating greater yellowness; WI represents whiteness, WI = 100 - [2 × (100 - L)]. 2 +a 2 +b 2 ] 0.5 C (chroma) represents chroma, and h (hue) represents hue. The higher the value, the more it is closer to a pure color.

[0079] like Figure 3 As shown, the L value of the animal blood cell polypeptide powder obtained in Example 5 was slightly higher than that of Comparative Examples 1-3, but there was no significant difference (P>0.05); the WI, C, and h values ​​of the animal blood cell polypeptide powder obtained in Example 5 were the highest, which were better than those of Comparative Examples 1-3 (P<0.05), confirming that the method of the present invention is more conducive to decolorization, the product is more likely to be pure in color, and it helps to maintain the stability of the product during storage.

[0080] The DPPH free radical scavenging ability of the animal blood cell polypeptide powders obtained in Example 5 and Comparative Examples 1-3 was determined. The specific operation was as follows: 1.5 mL of each group of animal blood cell polypeptide powder solutions with a concentration of 2 mg / mL was mixed with 1.5 mL of DPPH (1.0 mM), and the mixture was reacted at room temperature in the dark for 30 min. The absorbance was measured at 517 nm.

[0081] The superoxide anion scavenging ability of the animal blood cell polypeptide powders obtained in Example 5 and Comparative Examples 1-3 was determined. The specific operation was as follows: 0.1 mL of each group of animal blood cell polypeptide powder aqueous solution with a concentration of 2 mg / mL was added to 2.8 mL of Tris-HCl-EDTA (pH=8.2) buffer, incubated in a water bath at 25℃ for 10 min, and then 0.1 mL of 3.0 mM pyrogallol solution was added. The mixture was quickly mixed, and the absorbance was measured at 325 nm every 30 s for 5 min.

[0082] Superoxide anion scavenging capacity (%) = (V 对照 -V 试样 )÷V 对照 ×100%

[0083] Among them, V 对照 The auto-oxidation rate of pyrogallol in the control group (ΔA / min), V 试样 The oxidation rate of phthalic acid triterpenoids in each group is ΔA / min.

[0084] like Figure 4 As shown, the animal blood cell polypeptide powder obtained in Example 5 had the highest DPPH free radical scavenging capacity and superoxide anion scavenging capacity, which was superior to Comparative Examples 1-3 (P<0.05).

[0085] The reducing power of the animal blood cell polypeptide powders obtained in Example 5 and Comparative Examples 1-3 was determined as follows: 1.0 mL of the sample was mixed with 1.0 mL of sodium phosphate solution (pH = 6.6) and 1.0 mL of 1% potassium ferricyanide, and incubated at 50°C for 20 min. 1.0 mL of 10% TCA solution was added, and the mixture was centrifuged at 5000 × g for 10 min. 2.0 mL of the supernatant was taken and mixed with 2.0 mL of deionized water and 0.4 mL of 0.1% ferric chloride, and allowed to stand at room temperature for 10 min. The absorbance was measured at 700 nm.

[0086] The metal ion chelating ability of the animal blood cell polypeptide powders obtained in Example 5 and Comparative Examples 1-3 was determined, and the specific operation was as follows:

[0087] Add 50 μL of sample to 100 μL of 20 μM ferrous chloride solution, mix well, then add 100 μL of 0.5 mM phenoxyazine solution, shake vigorously until homogeneous, and measure the absorbance A at 562 nm after reacting for 10 min. 试样 Use 1.0 mL of deionized water instead of the sample to determine A. 对照 EDTA was used as a positive control, and all measurements were averaged three times.

[0088] Metal ion chelating ability (%) = [(A 对照 -A 试样 ) / A 对照 ×100%.

[0089] like Figure 5 As shown, the animal blood cell polypeptide powder obtained in Example 5 had the strongest reducing power and metal ion chelating ability, which was superior to Comparative Examples 1-3 (P<0.05).

[0090] The applicant believes that the above results are due to the following: The present invention involves freezing fresh animal blood to induce a large-scale lysis and fragmentation of blood cells, followed by homogenization, resulting in excellent fragmentation. Furthermore, the use of anthocyanins and polylysine effectively inhibits oxidation and denaturation during freezing and suppresses ice crystal damage to proteins, thereby increasing protein content and effectively ensuring the antioxidant activity of the peptides. Additionally, the invention uses animal blood fluid combined with rare-earth lanthanum chitosan chelate salt, which is then enzymatically hydrolyzed by a complex protease to form peptides with smaller molecular weights. These peptides have a uniform molecular weight distribution, good solubility, and excellent antioxidant activity.

[0091] The above merely describes preferred specific embodiments of the present application, but the protection scope of the present application is not limited thereto, and any person skilled in the art, according to the technical solution and inventive concept of the present application, makes equivalent replacement or change within the technical range disclosed by the present application, which should be covered within the protection scope of the present application.

Claims

1. A method for preparing animal blood cell polypeptide powder, characterized in that, The method comprises the following steps: S1, adding trisodium citrate into fresh animal blood and stirring for 10-30 min, adding cyanidin and polylysine and stirring uniformly, placing in a temperature environment of-10 to-20℃ for 1-2 h, increasing to room temperature, homogenizing for 1-5 min, centrifuging, removing supernatant, and obtaining animal blood corpuscle liquid; S2, adding the animal blood corpuscle liquid and rare earth lanthanum chitosan amine chelate salt into a glycine-sodium hydroxide buffer solution, stirring at 40-50℃ for 10-30 min, and adding complex protease and continuing to stir for 10-20 h to obtain an enzymatic hydrolysate; In S2, the specific activity of the complex protease is 5000-8000 U / g; the complex protease comprises at least one of bread yeast alkaline protease, serine alkaline protease, bacillus subtilis alkaline protease and streptomycin alkaline protease; S3, adjusting the pH value of the enzymatic hydrolysate to 5-6, increasing the temperature to 90-100℃ for 5-10 min, decreasing to room temperature, centrifuging for 5-10 min, and taking supernatant for low-temperature spray drying.

2. The method for preparing animal blood cell polypeptide powder according to claim 1, characterized in that, In S1, the fresh animal blood is one of chicken blood, duck blood, pig blood, cow blood and sheep blood.

3. The method for preparing animal blood cell polypeptide powder according to claim 1, characterized in that, In S1, the mass ratio of the fresh animal blood, trisodium citrate, cyanidin and polylysine is 100:1-3:1-2:0.1-1.

4. The method for preparing animal blood cell polypeptide powder according to claim 1, characterized in that, In S1, the increasing speed to room temperature is 1-2℃ / min; and the homogenizing speed is 8000-10000 r / min.

5. The method for preparing animal blood cell polypeptide powder according to claim 1, characterized in that, In S2, the mass ratio of the animal blood corpuscle liquid, rare earth lanthanum chitosan amine chelate salt, glycine-sodium hydroxide buffer solution and complex protease is 30-50:1-2:200-300:0.1-1; wherein the concentration of the glycine-sodium hydroxide buffer solution is 0.01-0.02 mol / L, and the pH value is 9-9.

6.

6. The method for preparing animal blood cell polypeptide powder according to claim 1, characterized in that, In S3, the pH value of the enzymatic hydrolysate is adjusted to 5-6 by using 1-2 mol / L hydrochloric acid.

7. The method for preparing animal blood cell polypeptide powder according to claim 1, characterized in that, In S3, the centrifuging speed is 5000-8000 r / min.

8. The method for preparing animal blood cell polypeptide powder according to claim 1, characterized in that, In S3, the low-temperature spray drying temperature is 40-50℃.

9. An animal globin polypeptide powder, characterized in that, The animal blood corpuscle polypeptide powder is prepared by the method in any one of claims 1-8.

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