5 results about "Pre column derivatization" patented technology

This invention relates to the field of detection and analysis technology, and discloses a pre-column derivatization-liquid chromatography-tandem mass spectrometry (LC-MS / MS) method for the detection of perfluorocarboxylic acids and their substitutes. The highly sensitive and selective method provided by this invention, based on 3-nitrophenylhydrazine pre-column derivatization-LC-MS / MS, achieves good correlation (R0) of nine target analytes (C2-C9 perfluorocarboxylic acids and GenX) within a wide linear range of 0.0001-100 ng / mL. 2 The method has a detection limit as low as 0.83 ng / L and a quantitation limit of 2.76 ng / L, with a sensitivity of 0.83 ng / L for short-chain trifluoroacetic acid, significantly better than traditional gas chromatography. The spiked recoveries in ultrapure water matrix range from 88.2% to 115.7%, with inter-day and intra-day precisions of 0.2% to 17.61% and 3.5% to 16.3%, respectively, demonstrating good accuracy and reproducibility. This method incorporates highly polar short-chain perfluorocarboxylic acids, previously only analyzable by GC-MS, into the LC-MS / MS detection system. It also achieves trace detection of the novel pollutant GenX at the ng / L level, with significantly higher sensitivity than existing methods.

The application discloses application of a phenylpropionic acid compound in detection of monosaccharide composition of traditional Chinese medicine polysaccharide. The phenylpropionic acid compound has a structure as shown in formula (I). The application provides a new application of the phenylpropionic acid compound, and preliminary realization of quality control research on traditional Chinese medicine preknowing polysaccharide. Compared with a method for detecting monosaccharide composition of traditional Chinese medicine polysaccharide by using 1-phenyl-3-methyl-5-pyrazolone as a pre-column derivatization reagent, the application has the following advantages: 1. Derivatization of aldehyde sugar and ketose can be simultaneously realized; 2. The reaction temperature is more moderate, and the safety of the reaction is greatly improved; 3. The detection wavelength of HPLC-UV is improved from 245 nm to 274 nm, interference is reduced, and sensitivity is improved; and 4. The pre-column derivatization reagent is low in cost and easy to obtain.

This invention discloses an online detection method and system for health hazard factors in drinking water based on pre-column derivatization, belonging to the field of water quality testing technology. The method includes: introducing a drinking water sample into a microfluidic chip-type derivatization reaction cell, where it reacts with a pH / temperature dual-response intelligent derivatization reagent to generate characteristic spectral derivatized products; acquiring spectral data using a micro fiber optic spectrometer, and calculating the derivatization efficiency in real time using a PID dynamic control model, automatically adjusting the reagent dosage and reaction conditions until the target is met; the target derivatized products are separated and purified twice by a two-dimensional chromatographic separation component and then transmitted to a dual internal standard correction machine learning model, where peak shape analysis, overlapping peak decomposition, dual internal standard correction, and quantitative regression calculations are performed to output the detection results and feed them back to the PID dynamic control model to update parameter thresholds. This invention improves the specificity, stability, and accuracy of the detection.

The application discloses a kind of brain tissue and blood plasma double matrix amino acid class matter pretreatment and detection method, the method includes the following steps: standard preparation;Differential sample preparation and homogenization;Double protein precipitation and preliminary purification;Solid phase extraction (SPE) depth purification and enrichment;Microenvironment control's pre-column derivatization;HPLC-FLD chromatographic separation and detection;Result calculation.The application is by optimizing sample pretreatment process, introduce SPE depth purification and pre-column derivatization technology under specific pH condition, effectively solve the problem of brain tissue and blood plasma matrix complex, many interferents and amino acid derivatization efficiency low.This method has the advantages of simple steps, high accuracy, good sensitivity and excellent reproducibility, can realize the rapid, synchronous detection of 13 kinds of amino acid class matters in rat brain tissue and plasma, provides reliable technical means for neurotransmitter and related metabolite research.

The present application relates to the technical field of amino acid detection, and specifically relates to a method for quantitatively detecting free amino acids in a polypeptide drug starting material protective amino acid, wherein the liquid chromatography conditions are as follows: the chromatographic column is AQ-C18, the mobile phase A is 0.1+ / -0.02% formic acid aqueous solution, the mobile phase B is 0.1+ / -0.02% formic acid acetonitrile solution, and the diluent is acetonitrile aqueous solution; the CAD detector conditions are as follows: the evaporation temperature is 50+ / -2 DEG C, and the gas adjustment mode is the analysis mode; and the free amino acid content is calculated by substituting the peak area of the amino acid reference solution and the peak area of the Fmoc protective amino acid solution into the external standard method. Compared with the pre-column derivatization-high performance liquid chromatography method, the present application does not need expensive derivatization reagents and complex derivatization processes; compared with the ultra-high performance liquid chromatography-tandem mass spectrometry method, the present application does not need to configure expensive mass spectrometers, has good economic efficiency and practical value, and can quantitatively detect the free amino acid content.