Method for determining lignocelluloses component content of plant straw

Abstract

The invention provides a method for determining the lignocelluloses component content of plant straw, and relates to the field of agronomy. The method particularly comprises the following steps: firstly, selecting the plant straw of a tested material for pretreatment; then carrying out two-step acidolysis respectively by adopting 72% concentrated sulfuric acid and 4% dilute sulfuric acid, and degrading and separating a lignocelluloses component in the plant straw; then accurately determining the content of cellulose and semi-cellulose by adopting an HPLC method, determining the content of acid-soluble lignin in a degraded liquid by adopting a UV method, and determining the content of acid-insoluble lignin by adopting a firing method. The method remedies the defects of insufficient pretreatment, extensive treatment process and inaccurate quantitative determination method caused by adopting a traditional method, gets a new breakthrough of determining the content of semi-fibers of lignin of the plant straw by adopting the HPLC method, can be used for accurately measuring the content of the lignocelluloses component in various plant straw, is accurate and reliable in result, and has wide reference and use values in the fields of agricultural crop production, biochemical analysis, biomass novel energy utilization and the like.

Description

technical field [0001] The invention relates to the field of agronomy, and specifically provides a method for measuring the content of lignocellulose components in plant straws. Background technique [0002] Plant straw lignocellulose mainly includes three components of cellulose (38%-50%), hemicellulose (23%-32%) and lignin (15%-30%) (Rubin, 2008), which constitute the The basic skeleton of the plant cell wall supports and protects the plant. The content of cellulose, hemicellulose and lignin in the plant straw is directly related to the mechanical strength of the plant, lodging resistance and other agronomic traits; the lignocellulose in the plant straw The components can be directly burned to generate heat, generate electricity, or be converted into ethanol, synthetic gas, and hydrogen after degradation (Xie Guanghui et al., 2007). It is a renewable resource with abundant sources that can be used to produce alternative energy (Himmel et al., 2007), the content of cellulo...

AI Technical Summary

Problems solved by technology

At present, the determination of plant straw lignocellulose content is mainly divided into material pretreatment (chemical method, physical method, biological method and physical chemical method) and quantitative determination (gravimetric method, concentrated acid hydrolysis sugar determination method, ultraviolet spectrophotometry and isotope method). Marking method, etc.), the predecessors have carried out the determination of the lignocellulose content of corn, rice and other food crops, and biomass energy crops such as miscanthus, sugarcane, etc., because of insufficient sample pretreatment, extensive operation process, and later quantitative determination The method is imprecise or even erroneous, resulting in large errors in the final measurement results, and cannot be used universally in various plant straws

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