Asiaticoside and preparation thereof

A technology of madecassoside and centella asiatica, applied in the field of asiaticoside and its preparation, to achieve the effects of simple process, low production cost and short production cycle

Inactive Publication Date: 2008-12-17
卢照凯 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN1491656A discloses the process steps of extracting Centella asiatica extract from Centella asiatica, but the main components of the extract are asiaticoside and madecassoside, and the content of the two in the extract is 10-95% , without separating asiaticoside and madecassoside

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1 of the present invention: the preparation method of asiaticoside, its steps are as follows:

[0018] 1) Extraction: put the fresh Centella asiatica into a clean extraction tank after crushing it, add 8 times the weight of 60% (v / v) ethanol, control the temperature at 85°C, and extract for the first time under reflux for 2 hours (timed from the boiling of the solution) ), then squeeze out the liquid medicine, add 6 times the weight of 60% (v / v) ethanol for the second time, reflux for extraction for 2 hours, and combine the extracts.

[0019] 2) Concentration and centrifugation: the extract is introduced into a clean concentrator for vacuum concentration, the temperature is controlled at 50°C, the vacuum is 0.08MPa, and it is concentrated to 1 / 6 of the volume of the extract; the concentrated extract is centrifuged through a three-legged Machine centrifuge, the speed is 1700r / min;

[0020] 3) Purification by column chromatography: the centrifuged supernatant ...

Embodiment 2

[0025] Embodiment 2 of the present invention: prepare asiaticoside according to the following steps:

[0026] 1) Extraction: crush the dried centella asiatica and put it into a clean extraction tank, add 12 times the weight of water, control the temperature at 100°C, reflux and extract for 3 hours for the first time (starting from the boiling of the solution), and then press out the liquid medicine , add 5 times the weight of water for the second time, reflux for extraction for 2 hours, add 2 times the weight of water for the third time, reflux and extract for 1 hour, and combine the extracts.

[0027] 2) Concentration and centrifugation: the extract is introduced into a clean concentrator for vacuum concentration, the temperature is controlled at 60°C, the vacuum is 0.1MPa, and it is concentrated to 1 / 2 of the volume of the extract; the concentrated extract is centrifuged through a three-legged Machine centrifuge, the speed is 1700r / min;

[0028] 3) Purification by column ch...

Embodiment 3

[0033] Embodiment 3 of the present invention: prepare asiaticoside according to the following steps:

[0034]1) Extraction: crush fresh Centella asiatica and add 2 to 3 times the weight of 80% (v / v) methanol to infiltrate for 6 hours to fully expand the raw Centella asiatica, put it into a percolator evenly in stages, press Tight, place for 8-48 hours and then add 17-18 times the weight of 80% (v / v) methanol, heat to 50 ° C to start percolation, percolation rate is 1BV / h, time 15 hours, collect percolation liquid.

[0035] 2) Concentration and centrifugation: the percolation liquid is introduced into the concentrator for vacuum concentration, the temperature is controlled at 40°C, the vacuum degree is 0.06MPa, and it is concentrated to 1 / 10 of the volume of the extract; the concentrated extract is centrifuged through a centrifuge, The speed is 1700r / min;

[0036] 3) Purification by column chromatography: the centrifuged supernatant is directly poured into a D101 macroporous a...

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PUM

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Abstract

The invention discloses an asiaticoside and a preparation method thereof. The Centella asiatica is adopted as the raw material and is extracted by water or low-carbon ethanol; the extract is separated and purified by column chromatography, the resin is decolored, and the extract is crystallized, recrystallized and dried and finally the extractive mainly composed of by the asiaticoside is obtained, wherein, the weight of the asiaticoside acounts for not less than 95 percent. The preparation method utilizes the modern biological extraction technology and adopts the technology processes of aqueous extract or ethanol extract, separation and purification by column chromatography, decoloring, crystallization and recrystallization to extract the asiaticoside from the hydrocotyle asiatica; the purity of the asiaticoside is improved by the resin column chromatography and recrystallization to lead the extractive contain more than 95 percent of the asiaticoside (determined by HPLC method); in addition, the technology process is simple, the production period is short, the consumption of organic solvent is less, and the production cost is low, thus being applicable to the commercial production.

Description

(1) Technical field: [0001] The invention relates to asiaticoside and a preparation method thereof, belonging to the technical field of extraction of medicinal active ingredients. (two) background technology: [0002] Centella asiatica is the whole herb or rooted whole plant of Centella asiatica, a dicotyledonous plant Umbelliferae. It has the effects of clearing away heat and dampness, reducing swelling and detoxification; Injury etc. Asiaticoside is one of the active ingredients in Centella asiatica extract. Madecassoside has multiple pharmacological effects: (1) Beauty: It has anti-oxidation effect, removes old dead fat layer, promotes skin metabolism, increases skin elasticity and smoothness, and supplements nutrients. (2) Health care: regulate endocrine, improve intestinal function, delay aging, improve memory, improve anemia, promote sleep, and improve gastrointestinal function. (3) Weight loss: lower cholesterol, remove excess fat and reduce overweight, regulate ne...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00A61K36/23
Inventor 卢照凯卢昶年
Owner 卢照凯
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