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Triterpene saponin in camellia seeds, preparation method and medical use thereof

A technology for triterpenoid saponins and seeds, which is applied in the field of medicine and achieves the effects of clear structure, high purity and high yield

Inactive Publication Date: 2009-03-25
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the extraction of total saponins from camellia seeds and the production and processing of its active ingredients into preparations

Method used

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  • Triterpene saponin in camellia seeds, preparation method and medical use thereof
  • Triterpene saponin in camellia seeds, preparation method and medical use thereof
  • Triterpene saponin in camellia seeds, preparation method and medical use thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Preparation of Total Camellia Saponins

[0047] Gained tea seed cake 10kg after getting dry camellia seed oil expression, remove impurity, dust.

[0048] Add medicinal materials and 10 times the amount of water in the traditional Chinese medicine extraction tank, heat to 80°C, extract twice, each time for 2 hours, combine the extracts and concentrate to 4 times the volume of the medicinal materials.

[0049] The extract was adsorbed by the macroporous resin, washed with 20% ethanol to remove impurities, and then eluted with 90% ethanol to obtain 680 grams of crude total saponins of camellia seeds (content 60%).

[0050] The obtained camellia seed crude total saponins are dissolved in 5 times the amount of water, and through open ODS column chromatography (sample size: filler 1:10), the tea flavonoid impurities are washed away with 20% ethanol, and then eluted with 85% ethanol to obtain the total camellia seed saponins 380 grams (content 85%).

[0051] The obtained cam...

Embodiment 2

[0053] Preparation of Total Camellia Saponins

[0054] (1) Take 6 kg of tea seed cake obtained after oil extraction from dried camellia seeds, and remove impurities and dust.

[0055] (2) Add medicinal materials and 8 times the amount of 95% ethanol in the traditional Chinese medicine extraction tank, heat to 80° C., extract twice for 1.5 hours each time, combine the extracts and concentrate to 2 times the volume of the medicinal materials.

[0056] (3) The extract was absorbed by the macroporous resin, washed with 30% ethanol to remove impurities, and then eluted with 90% ethanol to obtain 770 grams of crude total saponins of camellia seeds (content 65%).

[0057](4) Add 4 times the amount of water to dissolve the crude total saponins of the obtained camellia seeds, go through open ODS column chromatography (sample size: filler 1:12), wash off the tea flavonoid impurities with 20% ethanol, and then elute with 90% ethanol to obtain camellia Seed total saponins 430 grams (cont...

Embodiment 3

[0060] Effect of total saponins from camellia seeds on tumor growth in S180 tumor-bearing mice

[0061] Experimental animals: 8-week-old male ICR mice with an average body weight of 20 grams. Provided by the Experimental Animal Center of Zhejiang University.

[0062] Drugs and tumor strains: total saponins of camellia seeds were prepared according to the method described in claim 2; cyclophosphamide (CTX), 200 mg / bottle, produced by Shanghai Hualian Pharmaceutical Co., Ltd., and S180 ascites tumor mice were obtained from the Animal Experiment Center of Zhejiang University supply.

[0063] Preparation of S180 ascites tumor cell suspension: Operate under sterile conditions, take S180 sarcoma ascites type tumor-bearing mice inoculated for 10 days, disinfect the abdominal skin of the animal, penetrate the abdominal cavity with a 5mL disposable sterile syringe, and absorb the well-grown tumor cells. Ascites, the pumped ascites was put into a sterile Erlenmeyer flask and placed on...

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Abstract

The invention pertains to the field of medical technology and provides triterpenoid saponin in camellia seeds, and a preparation method and medical application thereof. The triterpenoid saponin is shown in a general formula (1), wherein, R1 is hydrogen or oxhydryl or acyloxy; R2 is the hydrogen or the acyloxy; R3 is the hydrogen or the acyloxy; R4 is the hydrogen or the acyloxy; R5 is methyl or methylol or aldehyde group or carboxyl or metheyl carboxyl; R6 is the hydrogen or orglycosyl; the preparation method comprises the following steps: macroporous resins are degreased, extracted by ethanol, decocted in water, desugared and decolored to obtain crude total saponins and then go though an opened ODS chromatographic column and repeated HPLC method to obtain the total saponins of camellia seeds and monomer theasaponin. The triterpenoid saponin compounds in the camellia seeds have the functions of protecting gastric mucosa, being antineoplastic, reducing blood sugar and blood fat and thelike and are used for preparing medicaments or health-care foods having the functions of protecting gastric mucosa, being antineoplastic and reducing blood sugar and blood fat.

Description

Technical field: [0001] The invention belongs to the technical field of medicine, and relates to triterpene saponins in camellia seeds, a preparation method thereof and a medical application thereof. Background technique: [0002] Camellia sinensis is a plant of the genus Camellia in the family Theaceae, which is widely cultivated in my country and is an excellent economic crop. There are systematic research reports on the chemical composition and biological activity of its young buds-tea. According to the existing research data, there are as many as 600 kinds of chemical components in tea, including more than 500 kinds of organic compounds. The biochemical reaction pathways of their synthesis and transformation are interrelated and mutually restrictive. Its biological activity involves: anti-inflammation, anti-allergy, anti-oxidation, scavenging free radicals, lowering blood sugar, lowering blood fat, promoting gastrointestinal tract, protecting liver, preventing precance...

Claims

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Application Information

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IPC IPC(8): C07J63/00A61K31/704A61K36/82A61P1/04A61P35/00A61P3/10A61P3/06
Inventor 李宁李铣张鹏马忠俊
Owner SHENYANG PHARMA UNIVERSITY
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