Method for simultaneously determining contents of chlorogenic acid, caffeic acid in dandelion preparation by HPLC

A technology of dandelion and chlorogenic acid, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that cannot fully reflect the quality of dandelion preparations, and achieve the effects of high sensitivity, simple method, and high recovery rate

Inactive Publication Date: 2008-12-17
HENAN UNIV OF CHINESE MEDICINE
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Problems solved by technology

Chlorogenic acid is also the main antibacterial component in dandelion. Only the content of caffeic acid can not fully reflect the quality of dandelion preparations. Therefore, a method for simultaneous determination of chlorogenic acid and caffeic acid in dandelion preparations was established. of great significance

Method used

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  • Method for simultaneously determining contents of chlorogenic acid, caffeic acid in dandelion preparation by HPLC
  • Method for simultaneously determining contents of chlorogenic acid, caffeic acid in dandelion preparation by HPLC
  • Method for simultaneously determining contents of chlorogenic acid, caffeic acid in dandelion preparation by HPLC

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Embodiment 1

[0028] 1. Instruments and reagents

[0029] LC-10AD (Shimadzu, Japan) high performance liquid chromatography, ultraviolet detector, HW-2000 chromatographic workstation; METTLER AE240 1 / 100,000 analytical balance, HANGPING JA2003 electronic balance, KQ-500DV numerical control ultrasonic cleaner ( Kunshan Ultrasonic Instrument Co., Ltd.); chromatographic methanol (Tianjin Siyou Fine Chemicals Co., Ltd.), water (Wahaha Purified Water), and other reagents were of analytical grade.

[0030] Caffeic acid and chlorogenic acid reference substances were produced by China Institute for the Control of Pharmaceutical and Biological Products, caffeic acid batch number: 110885-200102, chlorogenic acid batch number: 110753-200413. Dandelion medicinal materials were purchased from Zhengzhou Dongsheng Medicine Store (dandelion produced in Henan). Dandelion extract and dandelion slices are homemade.

[0031] 2. Methods and Results

[0032] 2.1 Chromatographic conditions and system suitabilit...

Embodiment 2

[0054] Methodological investigation

[0055] 1. Linear relationship inspection

[0056] Take by weighing an appropriate amount of caffeic acid reference substance, add methanol to make a 0.066mg / ml reference substance solution, inject 0.4, 0.8, 2, 3, 8 μl of samples respectively, and measure the peak area according to the chromatographic conditions in Example 1, and take the mass as The abscissa, the peak area is the ordinate, the standard curve is drawn, and the regression equation is calculated as: Y=1.444e+006X, r=0.9999, and there is a good linear relationship in the range of 0.0264 μg to 0.528 μg.

[0057] Weigh an appropriate amount of chlorogenic acid reference substance, add methanol to form a 0.0784 mg / ml reference substance solution, inject 0.4, 1, 2, 3, and 8 μl of samples respectively, and measure the peak area according to the chromatographic conditions in Example 1. The abscissa is the abscissa, the peak area is the ordinate, a standard curve is drawn, and the r...

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Abstract

The invention relates to a method for simultaneously measuring the content of chlorogenic acid and caffeic acid in a dandelion preparation by utilizing the HPLC method. The measurement method can effectively solve the problem that the chlorogenic acid and the caffeic acid are taken as the index components to simultaneously carry out the content measurement in order to lay the foundation for the quality standard of the dandelion preparation. The specific technical proposal comprises the following steps: respectively preparing a sample solution to be measured and a reference product solution, gradient-eluting with the RP-HPLC method by taking the methanol-phosphoric acid water as the mobile phase and measuring the content of the chlorogenic acid and the caffeic acid. The method has the advantages of convenience, reliability, rapidness, high sensitivity, good repeatability and high recovery rate, takes the chlorogenic acid and the caffeic acid as the index components, lays the foundation for the quality standard establishment of dandelion and the preparation thereof and provides more scientific basis.

Description

1. Technical field [0001] The invention relates to the field of quality detection of traditional Chinese medicines, in particular to a method for simultaneously determining the contents of chlorogenic acid and caffeic acid in dandelion preparations by HPLC. 2. Background technology [0002] Dandelion is a commonly used traditional Chinese medicine, and there is no uniform standard for its quality control. The Chinese Pharmacopoeia's quality control of dandelion is limited to the TLC identification and content determination of caffeic acid, and still adopts the quality control mode of western medicine, which cannot fully reflect the internal quality of dandelion. Although some people have used acetonitrile-0.2% phosphoric acid as mobile phase and room temperature gradient elution to determine the content of caffeic acid in dandelion in different regions, a rapid and stable HPLC method for quantitative analysis of caffeic acid in dandelion has been established. [0003] Howev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/34G01N30/06
Inventor 李喜凤朱艳琴陈随清董诚明孟璐师绘敏许闽
Owner HENAN UNIV OF CHINESE MEDICINE
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