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Determination method of effective component in aliphatic oil

The technology of an active ingredient and a determination method is applied in a field of determination of active ingredients in fatty oil, which can solve the problems of wasting organic solvents, adverse to human health, time-consuming and labor-intensive, and saves time, operation time and reagents. The effect of the solvent

Inactive Publication Date: 2006-04-12
NAT INST FOR THE CONTROL OF PHARMA & BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to this step, it takes about 4-6 hours to process a sample, which is time-consuming and labor-intensive, and wastes a lot of organic solvents, which is not good for human health and damages the environment.
Moreover, the traditional sample derivatization method has a low recovery rate of only about 83.6%, and the derivatization reagents also need to be used and prepared immediately, which brings inconvenience to the operation.

Method used

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  • Determination method of effective component in aliphatic oil
  • Determination method of effective component in aliphatic oil
  • Determination method of effective component in aliphatic oil

Examples

Experimental program
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Effect test

Embodiment 1

[0070] The assay method of the seal oil active ingredient described in the present embodiment, comprises the following steps:

[0071] 1) Derivatization treatment of seal oil:

[0072] Take 120 μl of seal oil, put it in a 10ml measuring bottle, add 4ml of 0.5mol / L sodium methoxide, shake well, react at room temperature for 15 minutes, until the small oil droplets completely disappear, then add 0.2ml of glacial acetic acid to terminate the reaction, and use 0.005% BHT Methanol constant volume, 0.2 μm filter membrane filtration, obtains the seal oil test solution that derivatization is processed;

[0073] 2) Get 20 μ l of the seal oil test solution after the derivatization treatment, and enter the liquid chromatograph, and the chromatographic conditions of the chromatograph are:

[0074] Chromatographic column: Lichrospher RP-18, 5μm, 100A, 4.6mm×250mm;

[0075] Mobile phase: acetonitrile: methanol: water = 7:1:2, isocratic elution;

[0076] Flow rate: 1.2ml / min;

[0077] Co...

Embodiment 2

[0083] The assay method of the seal oil active ingredient described in the present embodiment, comprises the following steps:

[0084] 1) Derivatization treatment of seal oil:

[0085] Take 80 μl of seal oil, put it in a 50ml measuring bottle, add 6ml of 0.8mol / L sodium methoxide, shake well, react at 40°C until the small oil droplets completely disappear, then add 0.3ml of glacial acetic acid to stop the reaction, and use 0.003% BHT containing Methanol constant volume, 0.2μm filter membrane filtration, obtains the seal oil test solution that derivatizes and handles;

[0086] 2) Get 30 μl of the seal oil test solution after the derivatization treatment, and enter the liquid chromatograph, and the chromatographic conditions of the chromatograph are:

[0087] Chromatographic column: LichrospherRP-18, 5μm, 100A, 4.6mm×250mm;

[0088] Mobile phase: methanol: water = 3: 2, isocratic elution;

[0089] Flow rate: 1.3ml / min;

[0090] Column temperature: 30°C;

[0091] Detection w...

Embodiment 3

[0096] The assay method of the seal oil active ingredient described in the present embodiment, comprises the following steps:

[0097] 1) Derivatization treatment of seal oil:

[0098] Take 100 μl of seal oil, put it in a 100ml measuring bottle, add 8ml of 1.0mol / L sodium methoxide, shake well, react at 50°C until the small oil droplets completely disappear, then add 0.5ml of glacial acetic acid to terminate the reaction, and use 0.001% BHT containing Methanol constant volume, 0.2μm filter membrane filtration, obtains the seal oil test solution that derivatizes and handles;

[0099] 2) Get 50 μ l of the seal oil test solution after derivatization, enter the liquid chromatograph, and the chromatographic conditions of the chromatograph are:

[0100] Chromatographic column: Waters SymmetryShield RP-18, 5μm, 100, 3.9mm×150mm;

[0101] Mobile phase: acetonitrile: methanol: water = 7:1:2, isocratic elution;

[0102] Flow rate: 1.3ml / min;

[0103] Column temperature: 15°C;

[0...

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Abstract

This invention discloses a test method for the effective components in fat oil, which applies the HPLC method to reduce the derivation operation steps of a sample and save time and reagents, not necessary to apply the step of extraction and applies suitable chromatograph conditions to separate the target components from impurities completely.

Description

technical field [0001] The invention relates to a method for determining effective components in fatty oils. Background technique [0002] Fur seals are deep-sea mammals that live in cold waters below the Arctic Circle at -50°C. They feed on rare cod, have a thick layer of fat under their skin, and are rich in omega-3 polyene fatty acids. The seal fat oil extracted from the seal fat of the pollution-free sea area in northern Canada contains three important omega-3 polyene fatty acids-EPA (Eicosapentaenoic Acid, Eicosapentaenoic Acid), DPA (Docosapentaenoic Acid, twenty Two carbon pentaenoic acid) and DHA (Docosahexaenoic Acid, docosahexaenoic acid), and the total content is more than 20%. After years of research in the medical field, it has been confirmed that these three fatty acids have a variety of unique biological activities on the human body: EPA can improve blood circulation, soften blood vessels, adjust blood lipids, lower blood pressure and blood sugar, and anti-in...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
Inventor 徐康森王召乐嘉静李湛君
Owner NAT INST FOR THE CONTROL OF PHARMA & BIOLOGICAL PROD
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