Method for separation purification of walnut green seedcase polyphenol substances by macroporous resin

A technology of walnut green skin and macroporous resin, applied in the field of food science, can solve the problems of high cost and low purity, and achieve the effects of simple operation, high quality and high efficiency

Inactive Publication Date: 2016-03-02
HENGSHAN COUNTY HONGMENGYUAN TECH IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional separation and purification methods such as precipitation separation and solvent extraction have shortcomings such as low purity or high cost. Macroporous resin method can make up for these shortcomings. It has simple process, convenient operation and low cost. It is widely used in food, medicine and cosmetics industries The application of the method has brought considerable economic benefits, but the application of the macroporous resin method in the separation and purification of walnut green bark polyphenols has not yet appeared

Method used

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  • Method for separation purification of walnut green seedcase polyphenol substances by macroporous resin
  • Method for separation purification of walnut green seedcase polyphenol substances by macroporous resin
  • Method for separation purification of walnut green seedcase polyphenol substances by macroporous resin

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Experimental program
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Effect test

Embodiment 1

[0047] 3.1 Preparation of sample solution: Weigh a certain amount of dried walnut green husk powder, use 50% ethanol to ultrasonically extract for 30 minutes at a temperature of 35°C and a solid-liquid ratio of 1g:17mL, and extract twice. The extract was vacuum filtered, and the filtrate was rotary evaporated to obtain a crude walnut green bark polyphenol extract, which was diluted to a corresponding concentration and refrigerated for later use.

[0048] 3.2 Determination of polyphenol content: Determination of the total polyphenol content in walnut green husk adopts the Folin-Ciocaileu method, pipette 0.2mL of crude extract, add it to a 10mL volumetric flask, add 0.5mL of FC reagent, mix well, and add it within 1min 1.5mL of 20% sodium carbonate solution, mix, and dilute to volume with deionized water;

[0049] After the above solution was placed in the dark at 35°C for 1.5h, the solution without crude extract was used as a blank control, and the absorbance value was measured...

Embodiment 2

[0068] 3.1 Preparation of sample solution: Weigh a certain amount of dried walnut green husk powder, use 50% ethanol to ultrasonically extract for 32.5min at a temperature of 37.5°C and a solid-liquid ratio of 1g:17mL, and extract twice. The extract was vacuum filtered, and the filtrate was rotary evaporated to obtain a crude walnut green bark polyphenol extract, which was diluted to a corresponding concentration and refrigerated for later use.

[0069] 3.2 Determination of polyphenol content: Determination of the total polyphenol content in walnut green husk adopts the Folin-Ciocaileu method, pipette 0.2mL of crude extract, add it to a 10mL volumetric flask, add 0.5mL of FC reagent, mix well, and add it within 1min 1.5mL of 20% sodium carbonate solution, mix, and dilute to volume with deionized water;

[0070] After the above solution was placed in the dark at 37.5°C for 1.75h, the solution without the crude extract was used as a blank control, and the absorbance value was me...

Embodiment 3

[0089] 3.1 Preparation of sample solution: Weigh a certain amount of dried walnut green husk powder, use 50% ethanol to ultrasonically extract for 35 minutes at a temperature of 40°C and a solid-liquid ratio of 1g:17mL, and extract twice. The extract was vacuum filtered, and the filtrate was rotary evaporated to obtain a crude walnut green bark polyphenol extract, which was diluted to a corresponding concentration and refrigerated for later use.

[0090] 3.2 Determination of polyphenol content: Determination of the total polyphenol content in walnut green husk adopts the Folin-Ciocaileu method, pipette 0.2mL of crude extract, add it to a 10mL volumetric flask, add 0.5mL of FC reagent, mix well, and add it within 1min 1.5mL of 20% sodium carbonate solution, mix, and dilute to volume with deionized water;

[0091] After the above solution was placed in the dark at 40°C for 2 hours, the solution without crude extract was used as a blank control, and the absorbance value was measu...

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Abstract

The invention discloses a method for separation purification of walnut green seedcase polyphenol substances by macroporous resin. The method comprises 1, carrying out extraction on walnut green seedcase dry powder at a temperature of 35-40 DEG C through ethanol with content of 50% under ultrasonic action many times, carrying out vacuum filtration on the extract, evaporating the filtrate to obtain walnut green seedcase polyphenol crude extract, diluting the walnut green seedcase polyphenol crude extract until a desired concentration is obtained, and carrying out refrigeration for next use, 2, determining total polyphenol content of walnut green seedcase by a Folin-Ciocaileu method, 3, carrying out macroporous resin pretreatment, 4, carrying out macroporous resin static adsorption and desorption, 5, carrying out macroporous resin dynamic adsorption and desorption, and 6, through a HPLC method, determining free phenol content and composition of the walnut green seedcase polyphenol extract obtained by the macroporous resin separation purification so that the whole separation purification process is finished. The method provides the novel approach for separation purification of walnut green seedcase polyphenol substances, can be operated simply and realizes high separation purification quality and efficiency.

Description

technical field [0001] The invention belongs to the field of food science, and in particular relates to a method for separating and purifying polyphenols in walnut green husks with a macroporous resin. Background technique [0002] In recent years, with the improvement of living standards, people's demand for health food is increasing day by day. Plants contain a large amount of polyphenols. These substances have various physiological functions such as anti-tumor, anti-oxidation, anti-radiation, anti-hyperglycemia, and hypolipidemic. They are important raw materials for health food. Polyphenols can be obtained from plants through separation and purification. phenolic substances. However, traditional separation and purification methods such as precipitation separation and solvent extraction have shortcomings such as low purity or high cost. The macroporous resin method can make up for these shortcomings. It has simple process, convenient operation and low cost. The applicat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/105
Inventor 张有林韩军岐张润光马乐
Owner HENGSHAN COUNTY HONGMENGYUAN TECH IND CO LTD
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