Method for determining radix notoginseng extract and contents of five types of ginsenosides in preparation of radix notoginseng extract by Fourier transform near-infrared spectrograph

Abstract

The invention discloses a method for determining a radix notoginseng extract and contents of five types of ginsenosides in a preparation of the radix notoginseng extract by a Fourier transform near-infrared spectrograph. The method comprises the steps that a sample is detected by an ultra-high performance liquid chromatography, moving phase builds acetonitrile and water gradient elution parameters with separation degrees higher than 1.7 in chromatographic peaks of Rg1 and Re, and the spectrum preprocessing adopts the combination of 2 to 3 methods in Savitzky-Golay polynomial smoothing, second-order differential, Noriis derivative filtering and data normalization; and three preferred wave bands of the five types of ginsenosides are modeled, and a calibration model is built by any one of a partial least squares regression method, a principal component regression method and a multiple linear regression method. The method is used for determining the contents of the five types of ginsenosides in the sample to be detected, the determining result is consistent with a result determined by the ultra-high performance liquid chromatograph basically, the requirements of 'Chinese pharmacopoeia' are met, the accuracy is high, and the determination speed is increased substantially.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a method for the rapid determination of five saponins (notoginseng saponin R 1 , Ginsenoside Rg 1 , Ginsenoside Re, Ginsenoside Rb 1 , ginsenoside Rd) content method. Background technique [0002] The notoginseng extract and the preparation thereof have the effects of increasing cerebral blood vessel flow, dilating cerebral blood vessels, improving hemodynamics, reducing cerebral ischemic brain tissue, inhibiting thrombus formation and the like. In the prior art, the determination of the content of active ingredients in the extract of Sanqi and its prepared preparations is based on the 2010 edition of the Chinese Pharmacopoeia and the quality standard of Xuesaitong injection WS3-B-3590-2001(Z)-2011 to detect the saponins of Sanqi R 1 , Ginsenoside Rg 1 , Ginsenoside Re, Ginsenoside Rb 1 , Ginsenoside Rd five component content, using high performance liquid chromatography (...

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Problems solved by technology

[0003] "Research on the Determination of Ginsenoside Rg1 and Re in Health Foods by UPLC" (Rong Weiguang et al., "Chinese Journal of Health Inspection", Volume 18, No. 11, November 2008, p2242-2243), although ultra-high performance liquid phase detection is used Ginsenoside Rg 1 and Re, but there is a lack of gradient elution parameters of the mobile phase of the key technology in the detection and the inability to detect five kinds of notoginseng saponins (the other three components Rd, R 1 , Rb 1 ) defects and deficiencies

[0004] Determination of ginsenoside Rg in American ginseng by ultra-high performance liquid chromatography 1 , Re, Rb 1 "(Yang Liwei et al., "Chinese Materia Medica", Volume 31, No. 1, January 2008, p55-57), although the conditions of ultra-high performance liquid chromatography are disclosed, but the gradient elution parameters are different from those in this patent At the same time, the results of ultra-high performance liquid chromatography were not compared with those of traditional liquid chromatography, and the consistency of the test results with national standards cannot be determined.

[0005] "A new method for rapid and non-destructive analysis of saponins in Chinese herbal medicine Panax notoginseng by near-infrared spectroscopy" (Yang Nanlin et al., "Acta Chemica Sinica" 2003, Vol. Near-infrared Spectroscopy Rapid Determination of Effective Components of Traditional Chinese Medicine" (Liu Quan et al., "Acta Chemical Industry" Vol. However, its basic data comes from conventional liquid chromatography. Due to the long analysis time of conventional liquid chromatography, the data deviation is large, which has a great impact on the chemical values ​​required for near-infrared modeling, and the lack of modeling technology makes The prediction results of the above literatures using near-infrared spectroscopy are not ideal

[0006] At present, the application of near-infrared technology to the content detection of the five components in the Panax notoginseng extract and its prepared preparations is the first, and there is no application report in this area at home and abroad

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