The invention discloses a method for three-dimensional representation of stratified structure micrometer CT imaging of a turbine blade heat barrier coating. The method comprises the following steps: preparing a double-layer-structure heat barrier coating micrometer CT scanning sample; firstly, carrying out micrometer CT scanning on the heat barrier coating sample, carrying out ring artifact and beam hardening correction on projection data, then carrying out three-dimensional reconstruction to obtain three-dimensional stratified structure information, establishing a three-dimensional structure analysis model, and carrying out characteristic segmentation and extraction on three components, namely a ceramic heat insulating layer, a bonding layer and matrix alloy; then, analyzing the thickness of the coating, extracting the distribution of holes in the coating, calculating the porosity, extracting the interface topography of the coating, and analyzing structure characteristics such as internal defects; and finally, estimating the preparation process quality of the heat barrier coating by integrating the composition, thickness, porosity distribution, interface topography and the like. The method for three-dimensional representation of the stratified structure of the heat barrier coating provided by the invention is suitable for estimation and optimization of the spraying process quality of the heat barrier coating, so that the quality of the heat barrier coating can be well controlled.