The invention discloses a portable lossless measuring device of plant nutrient components and a measuring method thereof; the measuring device comprises a monochromatic source, a photoelectric detector, a light source driving circuit, a signal conditioning circuit and a microcontroller system, and the monochromatic source and the photoelectric detector form an integral vane gripper that is respectively connected with the light source driving circuit and the signal conditioning circuit through electric cables, thus not only reducing light energy loss and raising signal-to-noise ratio, but also simplifying the measuring device and leading the sampling of the gripper to be flexible and convenient; the light source driving circuit mainly comprises a voltage regulating chip, a sampling resistance, a triode and an analog switch and realizes constant-current driving of the monochromatic source and programmed adjustment of current value, thus not only improving light source stability, but also effectively solving the saturation problem of the photoelectric detector, leading the photoelectric detector to work in the best detection range, increasing the signal-to-noise ratio and raising measuring precision; furthermore, the measuring device has additional measurement and deduction of dark noise in the measuring process, thus improving the measuring precision of the plant nutrient components.