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A kind of nano-medicine with tumor therapeutic effect and preparation method thereof

A nano-drug and tumor treatment technology, applied in the field of drugs with tumor treatment effect, can solve problems such as the inability to completely overcome drug resistance, and achieve the effects of overcoming drug resistance, reducing toxic and side effects, and having a good therapeutic effect.

Active Publication Date: 2018-03-02
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] So far, there have been many reports on hollow mesoporous silica nanoparticles (HMSNs) loaded with doxorubicin to treat cancer (Document 10: Gao Y, Chen Y, Ji X, et al.ACS nano, 2011 ,5(12):9788-9798; Literature 11: He Q, Gao Y, Zhang L, et al.Biomaterials, 2011,32(30):7711-7720), these methods can indeed improve the anticancer activity of the drug, However, the chemotherapeutic effect of chemotherapeutic drugs is always the main factor, and drug resistance cannot be completely overcome.

Method used

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  • A kind of nano-medicine with tumor therapeutic effect and preparation method thereof
  • A kind of nano-medicine with tumor therapeutic effect and preparation method thereof
  • A kind of nano-medicine with tumor therapeutic effect and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, DOX reacts with sodium bicarbonate, sodium hydroxide solution:

[0033] Take a certain amount of DOX and add it to 0.2mol / L sodium bicarbonate solution and sodium hydroxide solution with the same pH as the sodium carbonate solution, and let it stand.

[0034] see figure 1 , a is the action of DOX and sodium hydroxide solution, b is free DOX, and c is the action of DOX and sodium bicarbonate. Comparing a and c, it can be found that DOX can react with sodium bicarbonate to form a precipitate, which is bicarbonate; while NaOH cannot react with DOX to produce a precipitate.

Embodiment 2

[0035]Embodiment 2, preparation of hollow mesoporous silica nanoparticles (HMSNs):

[0036] Referring to literature (document 11 Yu Gao, Yu Chen, Xiufeng Ji, Xinyu He, Qi Yin, Zhiwen Zhang, Jianlin Shi, Yaping Li, ACS Nano, 2011, 5(12), 9788-9789)), under stirring conditions at 30°C, Mix 71.4mL ethanol, 3.14mL ammonia water and 10mL water evenly, then add 6mL tetraethoxysilane (TEOS) to react for 1h; pre-mix 5mL TEOS and 3mL octadecyltrimethoxysilane (C 18TMS), then quickly Add it into the above-mentioned reaction vessel, and continue to react for 1 hour; finally, disperse the prepared nanoparticles in a sodium carbonate solution (0.2M), and react in a water bath at 80° C. for 2 hours. The product was washed with ultrapure water, centrifuged, and finally calcined in a muffle furnace at 550°C for 6h to remove C 18TMS.

[0037] TEM characterization of HMSNs morphology and particle size:

[0038] Suspend an appropriate amount of HMSNs in ultra-pure water and disperse by ultraso...

Embodiment 3

[0040] Embodiment 3, the preparation of the nano-loading system (DMSNs) of loading doxorubicin:

[0041] Under dark conditions, disperse 100mg of HMSNs into DOX solution (1mg / mL, 10mL) and stir overnight, centrifuge the mixture at 8000rpm for 5min, remove the supernatant, wash the precipitate with ultrapure water three times and centrifuge to obtain DMSNs were ultrasonically dispersed in ultrapure water and stored at 4°C in the dark.

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PUM

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Abstract

The invention discloses a nano-medicine with tumor therapeutic effect and a preparation method thereof. It first loads doxorubicin with hollow mesoporous silica nanoparticles (HMSNs), and then treats it with bicarbonate to make a "nano bomb". When the drug enters the cell lysosome through endocytosis, the H+ in the lysosome can react with the drug and quickly generate CO2 gas; the generated gas destroys the integrity of the lysosome or improves the permeability of the lysosomal membrane Sex, induces lysosome-associated cell death, thereby killing cancer cells. The "nanometer bomb" also has a good therapeutic effect on drug-resistant strains, and can effectively overcome the drug resistance of cancer cells.

Description

technical field [0001] The present invention relates to a kind of medicament with function of treating tumor. Background technique [0002] According to the report of the International Agency for Research on Cancer (IARC), by 2030, there will be 21.4 million new cancer patients worldwide, and at the same time, 13.2 million new cancer patients will also die (Document 1: Allen T M, Cullis P R. Science, 2004 , 303(5665):1818-1822). [0003] Chemotherapy, referred to as chemotherapy, is one of the three major means of treating cancer, but the emergence of toxic side effects and multi-drug resistance of cancer cells has limited its application (Document 2: Luqmani Y A. Medical principles and practice: international journal of the Kuwait University, Health Science Centre, 2004, 14:35-48). [0004] A variety of methods have been used to solve the drug resistance of cancer cells, such as mitochondria-targeted photodynamic therapy (Document 3: Han K, Lei Q, Wang S B, et al. Advance...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/14A61K31/704A61K33/10A61K47/04A61P35/00
Inventor 郑鹄志杨利利
Owner SOUTHWEST UNIV
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