Method for carrying out lysosome classification by taking lysosome metabolite as marker

A technology for lysosomes and metabolites, applied in the field of lysosome classification, can solve the problem of poor fineness of lysosome classification
CN113267555AActive Publication Date: 2021-08-17UNIV OF SCI & TECH OF CHINA
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
UNIV OF SCI & TECH OF CHINA
Publication Date
2021-08-17

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Abstract

The invention discloses a method for carrying out lysosome classification by taking a metabolite of the lysosomes as a marker. The method comprises the following steps: obtaining contents of a single lysosome in a cell by adopting a single lysosome patch clamp technology; performing mass spectrometric detection on the lysosome content to obtain the composition and content of metabolites in the lysosome content; and classifying the lysosomes according to a detection result and differences of metabolite composition and content in a single lysosome content to obtain five different subgroups of lysosomes, including known autophagy lysosomes and endocytosis lysosomes. The lysosomes can be classified more finely, the lysosomes can be divided into five types by adopting the method, and each type of lysosomes has an obvious classification marker. Researchers provide clues for studying the specific functions of various lysosomes in the fields of aging and degenerative diseases such as development of drug metabolism for treating Alzheimer's disease and cancer.
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Description

technical field

[0001] The invention relates to the field of lysosome classification, in particular to a method for lysosome classification using lysosome metabolites as markers. Background technique

[0002] Lysosome is the main degradative organelle in cells and a key site of cell metabolism. Dysfunction of lysosomes is closely related to many diseases, such as lysosomal storage diseases, neurodegenerative diseases, tumors, etc. Therefore, lysosomes are also important targets for disease treatment and drug development. In addition, lysosomes are also involved in the intracellular transport, storage and metabolism of drugs, which are directly related to the resistance of multiple drugs. There are often a large number of lysosomes in cells, and these lysosomes have huge heterogeneity in size, shape, and function. Different lysosomes often play different roles in diseases and drug metabolism. The classification of enzymes and the study of the characteristics and differences...

Claims

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