A kind of ph-responsive lipid based on dendrimers and its preparation method and application

Abstract

The invention discloses a dendrimer-based pH-responsive lipid, a preparation method and application thereof, and belongs to the field of biological materials. The pH-responsive lipids involved in the present invention have different surface charges in different pH environments. The specific performance is that it presents negative, neutral or slightly positive electricity in the physiological environment in vivo, so that it can have good biocompatibility, and when it reaches the tumor tissue, it can utilize the pH difference between tumor tissue and normal tissue or inside and outside tumor cells. , the amino group is gradually exposed to expose more positive charges or realize charge reversal, and complete the contact between the positively charged carrier and the negatively charged cell membrane, thereby promoting the process of cell endocytosis. In cell lysosomes, cationic carriers continue to function as proton sponges or lysosomal membrane cleavage to escape from lysosomes.

Description

technical field

[0001] The invention relates to the field of biomedical materials, in particular to a dendrimer-based pH-responsive lipid and its preparation method and application. technical background

[0002] More and more gene-related diseases are considered to be curable by gene therapy, which usually requires the delivery of nucleic acid drugs to target cells to treat some tumors, asthma and cardiovascular diseases. But so far, the bottleneck limiting the clinical trials of gene drugs has been the development of safe and effective carriers. Given the high immunogenicity of viral vectors, non-viral vectors have become a hot topic for research. However, it is often difficult for non-viral vectors to obtain high transfection efficiency in vivo and in vitro. In order to improve the transfection ability of non-viral vectors, it is necessary to allow non-viral vectors to acquire the ability to respond to complex body environments like viruses. However, the research on non-...

Images