Preparation method for lysosomal membrane coated nanoparticle

A nanoparticle and lysosomal membrane technology, applied in medical preparations with non-active ingredients, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problems of low loading efficiency, loss, and damage to membrane structure biologically active proteins and other problems, to achieve the effects of high loading efficiency, economical savings, and uniform appearance
CN110711249AActive Publication Date: 2020-01-21BEIJING UNIV OF CHEM TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
BEIJING UNIV OF CHEM TECH
Publication Date
2020-01-21

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Abstract

The invention relates to a preparation method for a lysosomal membrane coated nanoparticle. Under the function of the driving of macrophages, an active lysosomal membrane coated nanoparticle is synthesized. Specifically, the preparation method comprises the following steps of: firstly, activating the macrophages by lipopolysaccharides, stimulating the secretion of lysosomal related proteases in the macrophages, and enhancing the enzymatic activity of the lysosomal related proteases; then, utilizing the phagocytic ability of the macrophages to jointly incubate the prepared nanoparticle and themacrophages to enable the macrophages to carry out phagocytosis on the nanoparticle, and carrying out internalization on the nanoparticle in macrophage lysosome; and finally, utilizing a lysosome extraction kit to extract the lysosome to obtain the lysosomal membrane coated nanoparticle. Compared with a preparation method for a traditional lipid membrane coated nanoparticle, on one hand, the preparation method disclosed by the invention is simple, and does not need to consider damage caused for the membrane by pressure or electric shock. On the other hand, the macrophages are taken as drivingforce, and the prepared lysosomal membrane coated nanoparticle keeps the activity of hydrolase in the lysosome.
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Description

Technical field:

[0001] The present invention relates to the preparation technology and method of lysosome-coated functional nanometer medicine. Under the action of macrophage drive, the nanoparticle coated with active lysosome membrane is synthesized. Specifically, macrophages are first activated with lipopolysaccharide to stimulate the secretion of lysosome-related proteases in macrophages and enhance their enzymatic activity, and secondly, using the phagocytic ability of macrophages, the prepared nanoparticles are co-incubated with macrophages , macrophages phagocytose the nanoparticles, internalize the nanoparticles in the macrophage lysosomes, and finally extract the lysosomes with a lysosome extraction kit to obtain nanoparticles coated with a lysosomal membrane. Background technique:

[0002] Due to the diversity, heterogeneity and recurrence of tumors, it is difficult to effectively inhibit tumor recurrence and metastasis by simply relying on traditional surgery and ...

Claims

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