Novel nano-drug with tumor treatment action and preparation method

A nano-medicine and tumor treatment technology, which is applied in the field of drugs with tumor therapeutic effects, can solve problems such as inability to completely overcome drug resistance, and achieve the effects of overcoming drug resistance, reducing toxic and side effects, and good therapeutic effects

Active Publication Date: 2015-10-21
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] So far, there have been many reports on hollow mesoporous silica nanoparticles (HMSNs) loaded with doxorubicin to treat cancer (Document 10: Gao Y, Chen Y, Ji X, et al.ACS nano, 2011 ,5(12):9788-9798; Literature 11: He Q, Gao Y, Zhang L, e...

Method used

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  • Novel nano-drug with tumor treatment action and preparation method
  • Novel nano-drug with tumor treatment action and preparation method
  • Novel nano-drug with tumor treatment action and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, DOX reacts with sodium bicarbonate, sodium hydroxide solution:

[0034] Take a certain amount of DOX and add it to 0.2mol / L sodium bicarbonate solution and sodium hydroxide solution with the same pH as the sodium carbonate solution, and let it stand.

[0035] see figure 1, a is the action of DOX and sodium hydroxide solution, b is free DOX, and c is the action of DOX and sodium bicarbonate. Comparing a and c, it can be found that DOX can react with sodium bicarbonate to form a precipitate, which is bicarbonate; while NaOH cannot react with DOX to produce a precipitate.

Embodiment 2

[0036] Embodiment 2, preparation of hollow mesoporous silica nanoparticles (HMSNs):

[0037] Refer to the literature (document 11Yu Gao, Yu Chen, Xiufeng Ji, Xinyu He, Qi Yin, Zhiwen Zhang, Jianlin Shi, Yaping Li, ACS Nano, 2011, 5(12), 9788-9789)), under stirring conditions at 30°C , mix 71.4mL ethanol, 3.14mL ammonia water, and 10mL water evenly, then add 6mL tetraethoxysilane (TEOS) to react for 1h; mix 5mL TEOS and 3mL octadecyltrimethoxysilane (C 18 TMS) was pre-mixed, then quickly added to the above reaction vessel, and continued to react for 1 h; finally, the prepared nanoparticles were dispersed in sodium carbonate solution (0.2M), and reacted in a water bath at 80° C. for 2 h. The product was washed with ultrapure water, centrifuged, and finally calcined in a muffle furnace at 550 °C for 6 h to remove C 18 TMS.

[0038] TEM characterization of HMSNs morphology and particle size:

[0039] Suspend an appropriate amount of HMSNs in ultra-pure water and disperse by ult...

Embodiment 3

[0041] Embodiment 3, the preparation of the nano-loading system (DMSNs) of loading doxorubicin:

[0042] Under dark conditions, disperse 100mg of HMSNs into DOX solution (1mg / mL, 10mL) and stir overnight, centrifuge the mixture at 8000rpm for 5min, remove the supernatant, wash the precipitate with ultrapure water three times and centrifuge to obtain DMSNs were ultrasonically dispersed in ultrapure water and stored at 4°C in the dark.

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Abstract

The invention discloses a novel nano-drug with a tumor treatment action and a preparation method. The preparation method comprises the steps of carrying adriacin doxorubicin with hollow mesoporous SiO2 nanoparticles (HMSNs), and then treating with bicarbonate to obtain nano-bomb. When drug enters cell lysosome by endocytosis, H<+> in the lysosome reacts with the drug and fast generates CO2 gas; the generated gas damages completeness of the lysosome or improves the permeability of a lysosome membrane, induces the death of cells related to the lysosome, therefore, cancer cells are killed. The nano-bomb also has well treatment effect for drug-resistant strains, and can effectively overcome tolerance of the cancer cells.

Description

technical field [0001] The present invention relates to a kind of medicament with function of treating tumor. Background technique [0002] According to the report of the International Agency for Research on Cancer (IARC), by 2030, there will be 21.4 million new cancer patients worldwide, and at the same time, 13.2 million new cancer patients will also die (Document 1: Allen T M, Cullis P R. Science, 2004 , 303(5665):1818-1822). [0003] Chemotherapy, referred to as chemotherapy, is one of the three major means of treating cancer, but the emergence of toxic side effects and multi-drug resistance of cancer cells has limited its application (Document 2: Luqmani Y A. Medical principles and practice: international journal of the Kuwait University, Health Science Centre, 2004, 14:35-48). [0004] A variety of methods have been used to solve the drug resistance of cancer cells, such as mitochondria-targeted photodynamic therapy (Document 3: Han K, Lei Q, Wang S B, et al. Advance...

Claims

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Application Information

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IPC IPC(8): A61K9/14A61K31/704A61K33/10A61K47/04A61P35/00
Inventor 郑鹄志杨利利
Owner SOUTHWEST UNIVERSITY
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