Yelk immune globulin products for preventing and treating bainite cryptosporidiosis and application thereof

A technology of yolk immunoglobulin and Cryptosporidium beinii, which is applied to allergic diseases, antibodies, anti-infective drugs, etc., can solve the problems that there is no application of yolk immunoglobulin for the prevention and treatment of Cryptosporidium beiii, and achieve The effect of low production cost

Inactive Publication Date: 2007-12-05
HENAN AGRICULTURAL UNIVERSITY
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Other also have the patent application that publication number is CN1435260, CN1439281, CN1250056, although disclose the ...

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  • Yelk immune globulin products for preventing and treating bainite cryptosporidiosis and application thereof
  • Yelk immune globulin products for preventing and treating bainite cryptosporidiosis and application thereof

Examples

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preparation example Construction

[0018] Preparation method of hyperimmune Cryptosporidium beinii IgY antibody

[0019] 1. Preparation of immune antigen

[0020] 1. Amplification and purification of Cryptosporidium beinii oocysts The oocysts in the positive feces of a certain animal husbandry station in the urban area were collected, and they were long oval in shape. x 4.1~5.5μm) shape index 1.31, cleared chicken coccidia oocysts mixed with 3-day-old quail blindly, established an animal infection model in our laboratory and compared 18sRNA sequence analysis, and determined that it was Cryptosporidium beinii ( After C.baileyi), 3-day-old healthy chicks were used to subculture to amplify the oocysts of Cryptosporidium beinii in large quantities.

[0021] According to the method of Arrowood et al., the oocysts were purified by sucrose gradient centrifugation. Sucrose stock solution recipe: 500g sucrose, dissolved in 320mL distilled water, autoclaved for later use; PBS buffer: NaCL 8.0g, KCL 0.2g, KH 2 PO 4 0...

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Abstract

The present invention provides yolk immunoglobulin preparation for preventing and treating cryptosporidiosis and health product of yolk immunoglobulin for strengthening immunity. The yolk immunoglobulin is prepared through the following steps: gathering cryptosporidium egg capsule from positive cryptosporidium excrement, separating, sucrose gradient centrifuging to purify cryptosporidium egg capsule and prepare cryptosporidium antigen, immunizing female laying fowl with the cryptosporidium antigen, collecting laid fowl egg, collecting yolk, diluting with distilled water, centrifuging to obtain supernatant, water diluting, and salting out with ammonium sulfate to obtain yolk immunoglobulin. The present invention is acted on the pathogen of cryptosporidiosis to reach specific inhibition.

Description

technical field [0001] The invention belongs to the field of biotechnology, relates to the technical field of aquatic animal breeding, and in particular relates to a kind of yolk immunoglobulin (IgY) for preventing and treating Cryptosporidiosis bainii and its application. Biological agents for primary diseases caused by sporozoites and health products for enhancing immune function. Background technique [0002] In 1907, Tyzzer discovered Cryptosporidium spp. for the first time in mouse mucosal tissue sections, and Cryptosporidiosis became a worldwide distributed zoonotic parasitic disease. The protozoan is widely parasitic on more than 180 species of humans and animals such as mammals, poultry, reptiles and amphibians, and can cause diarrhea in humans, especially fatal diarrhea in infants and young children and immunocompromised or deficient persons. Cause diarrhea and respiratory symptoms in livestock and poultry, resulting in decreased production performance or death. I...

Claims

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Application Information

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IPC IPC(8): A61K39/395A61P37/04A61P33/02
Inventor 张龙现邵兆霞宁长申菅复春赵金凤刘红英马超峰
Owner HENAN AGRICULTURAL UNIVERSITY
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