Method for purifying cryptosporidium protein kinase

A purification method and technology of Cryptosporidium, applied in the field of in vitro recombinant protein purification, can solve the problems of pure protein, unguaranteed activity, hinder systematic research on the invasion mechanism of Cryptosporidium, etc., and achieve the effect of improving solubility
CN109735513APending Publication Date: 2019-05-10EAST CHINA UNIV OF SCI & TECH +1
8 Cites 3 Cited by

Patent Information

Authority / Receiving Office
CN ยท China
Current Assignee / Owner
EAST CHINA UNIV OF SCI & TECH
Publication Date
2019-05-10

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a method for purifying cryptosporidium protein kinase. Firstly, an in-vitro recombination expression vector of cryptosporidium protein kinase is constructed; then plasmid transduction and IPTG low-temperature inducible expression are carried out; after successfully expressed thallus is split, protein is purified through rubber tapping recycling. According to the method forpurifying cryptosporidium protein kinase, by means of low-temperature inducing and IPTG concentration improving, the solubility of expression protein is improved, the problem that during in-vitro expression of cryptosporidium protein kinase, inclusion body expression is likely to autotomizing degradation is solved through a rubber tapping recycling method, high-purity single-strip recombinant protein with enzyme activity can be obtained, and the purifying effect on protein hard to express and large in molecular weight can be improved. The purified protein can be further used for functional analysis and studies, and a foundation is laid for solving various problems in the field of cryptosporidium protein functions.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of biotechnology, and more specifically relates to a method for purifying an in vitro recombinant protein of Cryptosporidium protein kinase. Background technique

[0002] Cryptosporidium ( Cryptosporidium ) is a widely distributed and serious pathogen of diarrheal diseases. Cryptosporidium is a parasitic protozoan of the Apicomplexan phylum, including more than 70 known species and genotypes. A disease that infects almost all vertebrates and causes diarrhea, especially persistent watery diarrhea in young animals. The infection rate of Cryptosporidium to young cattle, sheep and pigs is very high (>30%), and the cumulative infection rate to livestock within one year old is 100%. It is also the main pathogen of dairy cow diarrhea in many countries. Therefore, this disease causes serious harm to aquaculture.

[0003] In addition, Cryptosporidium is also an important zoonotic pathogen, and it is the second pathogen ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More