ISSR-SCAR (inter simple sequence repeat-sequence characterized amplified region) marker capable of identifying Wujiang brassica chinensis and identification method of marker

Abstract

The invention discloses an ISSR-SCAR (inter simple sequence repeat-sequence characterized amplified region) marker capable of identifying Wujiang brassica chinensis and an identification method of the marker. The method includes: performing total DNA (deoxyribose nucleic acid) extraction, PCR (polymerase chain reaction) amplification, primer selection and ISSR-SCAR detection on Wujiang brassica chinensis and other 10 brassica chinensis varieties, and finally determining an ISSR-SCAR molecular marker and a molecular identification method of the Wujiang brassica chinensis. A primer shown in the sequence such as SEQ ID NO:1 is used for amplification to obtain a specific fragment shown in the sequence such as SEQ ID NO:2. According to the sequence, a pair of primers in the sequences such as SEQ ID NO:3 and SEQ ID NO:4 is designed. The marker is simple to operate, stable and reliable, is the first molecular identification marker applied to the variety of Wujiang brassica chinensis, can be used for quickly identifying the variety and has significance of protecting germplasm resources of geographical indication products.

Description

technical field [0001] The invention belongs to the field of molecular marker identification, in particular to an ISSR-SCAR marker capable of identifying Wujiang fragrant green vegetables and an identification method thereof. Background technique [0002] Green vegetables( Brassica chinensis L.) is also called Chinese cabbage and non-heading Chinese cabbage. It belongs to the genus Brassicaceae. It is an annual or biennial herb and is an important vegetable and oil crop. Green vegetables are originally produced in China, but in recent years, some countries such as Southeast Asia, Europe and the United States have gradually introduced and cultivated them, and they have become a worldwide vegetable. Among them, Xiangqingcai is not only a unique green vegetable variety in my country, but also a rare vegetable variety with local traditional characteristics in Suzhou. It has been cultivated for more than 100 years. It is mainly planted along the East Taihu Lake and the south...

AI Technical Summary

Problems solved by technology

[0004] Although the current DNA molecular marker technology has achieved certain research results in green vegetables, it mainly focuses on genetic diversity and genetic map construction. Most of the reports on variety identification use SSR fingerprints to identify green vegetable varieties. This method is cumbersome to operate, has poor reproducibility, and is not a specific and specific molecular marker that can be directly applied, so it is not suitable for the development of a stable, rapid and accurate variety identification system

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