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Novel Klebsiella sp. or Candida sp. strain, and microbial fuel cell containing same

A Klebsiella and Candida technology, which is applied in the field of novel Klebsiella or Candida strains and microbial fuel cells containing the same, can solve problems such as no reports being published, and achieve high-efficiency wastewater treatment Fields, the effect of a wide range of applications

Active Publication Date: 2016-07-13
EWHA UNIV IND COLLABORATION FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, even in such a device that combines a sewage or wastewater treatment device and a microbial fuel cell, the performance is still determined by the efficiency of the microorganisms used, so there is an urgent need to develop a microorganism that is excellent in converting organic matter into hydrogen ions, But so far, no report has disclosed such microorganisms

Method used

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  • Novel Klebsiella sp. or Candida sp. strain, and microbial fuel cell containing same
  • Novel Klebsiella sp. or Candida sp. strain, and microbial fuel cell containing same
  • Novel Klebsiella sp. or Candida sp. strain, and microbial fuel cell containing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1: Screening and identification of bacterial strains for microbial fuel cells

[0056] To screen strains for microbial fuel cells present in anaerobic sludge, strains for microbial fuel cells were screened from the biofilm formed on the anode (Anode) after operating the microbial fuel cell using the anaerobic sludge and identify.

[0057] Specifically, the microbial fuel cell utilizes Singlechambersystems (Anode: carbonfelt (9×14 cm) as an anode electrode obtained by pretreatment at 450°C for 30 minutes; air-cathode (air-cathode, ODE75, MEET , Korea); Protonexchangemembrane: Nafion117 (Dupont, USA); Reactor: 7×15×10cm (L×H×W) persingle chamber (1-Lvolume); Voltagemonitoringsystem: digitalmultimeter (GL220, Japan); Line: titaniumwire (Sigma, USA )), wherein the anode electrode and cathode electrode included in the microbial fuel cell are connected to a platinum electrode and a 100Ω external resistor. In addition, as a culture solution for microbial fuel cells, ...

Embodiment 2

[0070] Example 2: Evaluation of microbial fuel cells utilizing Klebsiella sp. IR21 strain or Candida sp. IR11

[0071] The results of microbial fuel cells containing the Klebsiella IR21 or Candida IR11 strains identified in Example 1 were run and evaluated.

Embodiment 2-1

[0072] Example 2-1: Microbial culture environment and voltage changes

[0073] Specifically, among the colonies obtained in Example 1, bacterial cells were obtained from colonies corresponding to Klebsiella IR21 or Candida IR11 strains, inoculated into the inoculum culture solution, and then cultured to obtain Obtain cultures.

[0074] After mixing the culture and anaerobic sludge, the mixture was used as an initial inoculum source, and as a substrate a glucose and acetate mixture (1:1, w / w), or COD is about 15,000mg / L the waste water in the hydrogen production process, in addition, by the method identical with described embodiment 1, run microbial fuel cell ( Figure 3a , 3b , 4a and 4b).

[0075] In addition, the culture and anaerobic sludge were mixed, and the mixture was used as an initial inoculum source, and a glucose and acetate mixture (1:1, w / w), or COD is about the waste water in the biogas production process of 500mg / L, in addition, by the same method as descr...

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Abstract

The present invention relates to: a novel Klebsiella sp. IR21 or Candida sp. IR11 strain; a composition for wastewater treatment or wastewater purification, containing the same; a method for treating or purifying wastewater by using the composition; a microbial fuel cell containing the strain; a method for producing electricity by using the microbial fuel cell; a wastewater treatment apparatus comprising the microbial fuel cell; and a method for treating or purifying wastewater by using the wastewater treatment apparatus. The novel Klebsiella sp. IR21 or Candida sp. IR11 strain of the present invention allows the purification of wastewater exhibiting a remarkably high COD (Chemical Oxygen Demand) value and the production of electricity to be simultaneously promoted and allows a wastewater treatment apparatus to be operated without an external power source by designing a wastewater treatment apparatus comprising a microbial fuel cell, and thus could be widely applied to more effective wastewater treatment.

Description

technical field [0001] The present invention relates to a kind of novel Klebsiella genus or Candida bacterial strain and the microbial fuel cell comprising it, more particularly, the present invention relates to a kind of novel Klebsiella genus IR21 (Klebsiellasp.IR21) or Candida Candidasp. A method for generating electricity by a microbial fuel cell, a wastewater treatment device equipped with the microbial fuel cell, and a method for treating or purifying wastewater using the wastewater treatment device. Background technique [0002] A microbial fuel cell (MFC) is a device that converts chemical energy of a substrate serving as an electron donor into electrical energy by using microorganisms as a catalyst. Specifically, it refers to the following devices: including equipment that converts chemical energy generated during the decomposition of the substrate due to microbial metabolism into electrical energy, and equipment that recovers the converted electrical energy, and f...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/14C12N1/20C02F3/34H01M8/16C12R1/72C12R1/22
CPCC02F3/34C12N1/00C12N1/14C12N1/20H01M8/16Y02E60/50Y02P70/50C12R2001/22C12N1/205C12R2001/72C12N1/165
Inventor 赵敬淑金泰冠李润英
Owner EWHA UNIV IND COLLABORATION FOUND
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