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Compound bacterium preparation capable of efficiently adsorbing mercury and application of compound bacterium preparation

A technology for composite bacteria and mercury adsorption, which is applied in the application, restoration of contaminated soil, bacteria, etc., can solve the problems of mercury resistance performance to be improved, and the number of mercury resistant bacteria is small, so as to achieve the advantage of adsorption efficiency, strong mercury adsorption capacity, adaptable effect

Active Publication Date: 2021-06-15
西安中地环境科技有限公司 +1
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  • Abstract
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Problems solved by technology

[0005] The object of the present invention is to provide a kind of composite bacteria preparation and application of highly efficient adsorption of mercury, which is used to solve the problem that the number of mercury-resistant bacteria used in the bioremediation of mercury-contaminated soil is small and the mercury-resistant performance needs to be improved. Adsorption performance of effective mercury, preventing and controlling the migration of mercury from soil to plants

Method used

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  • Compound bacterium preparation capable of efficiently adsorbing mercury and application of compound bacterium preparation
  • Compound bacterium preparation capable of efficiently adsorbing mercury and application of compound bacterium preparation
  • Compound bacterium preparation capable of efficiently adsorbing mercury and application of compound bacterium preparation

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preparation example Construction

[0039] The preparation of composite bacteria preparation of the present invention may further comprise the steps:

[0040] (1) Klebsiella N-type strain and Bacillus tokyo K-type strain were obtained by isolation and purification from mercury-contaminated soil; Bacillus subtilis W-type strain was obtained by isolation and purification from microbial selenium-enriched bacterial agents; the isolation, Purification is carried out with mercury-containing media.

[0041] The above-mentioned separation and purification adopt the plate marking method, and repeated operations are performed. During the repeated operations, the Hg in the mercury-containing medium 2+ Concentrations increased sequentially; for Klebsiella type N strains, Bacillus tokyo K type strains, the initial Hg in the mercury-containing medium 2+ The concentration is 5mg / L, the highest Hg 2+ Concentration of 30-35 mg / L (eg 30 mg / L, 31 mg / L, 32 mg / L, 33 mg / L, 34 mg / L, 35 mg / L); for Bacillus subtilis type W strains, in...

Embodiment 1

[0054] The preparation method of the composite bacteria of the present embodiment may further comprise the steps:

[0055] (1) Isolation and domestication, isolation and purification of N-type bacteria, K-type bacteria and W-type bacteria

[0056] N-type strains and K-type strains were isolated from mercury-contaminated soil, and W-type strains were isolated from microbial selenium-enriched bacterial agents. The specific process is as follows:

[0057] ① Take 10g of soil with low, medium and high mercury pollution levels and place them in 250mL Erlenmeyer flasks, dilute with 100mL ultrapure water to make bacterial suspension, shake at 140r / min for 30min, let stand, and take 1mL supernatant;

[0058] Microbial selenium-enriched bacterial agent directly takes 1mL of bacterial liquid for subsequent processing.

[0059] ② Inoculate the obtained 3 supernatants into Hg-containing 2+ Streak culture was carried out in a solid plate medium with a concentration of 5 mg / L, and after st...

Embodiment 2

[0088] The difference between the preparation method of the composite bacterial preparation of this example and that of Example 1 lies in step (3), and the other steps are the same as those of Example 1, and will not be repeated here. Step (3) is as follows:

[0089] Pick one of the obtained N-type bacteria, K-type bacteria, and W-type bacteria for enrichment culture in TSB medium respectively, and obtain three kinds of enrichment solutions with OD values ​​between 0.5 and 0.6. The solution was mixed at a mass ratio of 0.8:1.0:1.2 to obtain a composite strain, and 5 mL of the composite strain was inoculated in TSB medium for cultivation. The initial pH of the cultivation was 7.08, the cultivation temperature was 30°C, and the cultivation time was 18 hours. During cultivation, the culture medium is shaken at a speed of 140 r / min. When the OD value is between 0.5 and 0.6, the cultivation ends, and the obtained composite bacteria solution is the composite bacteria preparation of ...

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Abstract

The invention belongs to the technical field of microbial agents, and particularly relates to a compound bacterium preparation capable of efficiently adsorbing mercury and application of the compound bacterium preparation. The preparation of the compound bacterium preparation comprises the following steps: (1) performing separation and purification in mercury-polluted soil to obtain Klebsiella pneumoniae and Bacillus toyonensis, and performing separation and purification in a microbial selenium-rich microbial agent to obtain Bacillus subtilis, wherein the separation and purification are carried out through a mercury-containing culture medium; and (2) performing enrichment culture on the Klebsiella pneumoniae, the Bacillus toyonensis and the Bacillus subtilis obtained in the step (1), performing mixing according to an enrichment liquid mass ratio of (0.8 to 1.2):(0.8 to 1.2):(0.8 to 1.2), and inoculating the mixed strains into a culture medium for culturing to obtain a culture solution which is the compound bacterium preparation. By using the adsorption performance of the composite bacterium preparation on the effective mercury, the mercury is prevented and controlled from migrating from soil to plants, three kinds of microbes in the compound bacterium preparation achieve a synergistic effect, efficient adsorption on mercury is realized, and the adsorption rate is obviously superior to that of a single strain.

Description

technical field [0001] The invention belongs to the technical field of microbial bacterial preparations, and in particular relates to a composite bacterial preparation for efficiently adsorbing mercury and its application. Background technique [0002] The serious harm caused by mercury pollution has attracted the attention of all countries in the world, and the degree of concern is increasing. At present, more than 130 countries have formed the United Nations "Minamata Disease" Committee to specifically regulate the emission and use of mercury. The U.S. Environmental Protection Agency has formulated standards for emissions into the atmosphere, water, and waste, as well as related standards for emissions in industrial production (such as air protection bills, water purification bills, resource protection and recycling bills). In this regard, the China Council for International Cooperation in Environmental Development has studied and formulated special policies to regulate th...

Claims

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Application Information

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IPC IPC(8): C12N1/20C09K17/14B09C1/10C12R1/22C12R1/125C12R1/07
CPCC12N1/20C09K17/14B09C1/10
Inventor 孙芳强荆秀艳谢金艳王晓娟马冬冬齐佳华张文媛卢彤董强飞
Owner 西安中地环境科技有限公司
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