Compound microbial preparation for degrading phenol endocrine disrupter and preparation method

An endocrine disruptor, compound microorganism technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of neglecting the safety detection of secondary pollution of sludge, single enzyme system, and inability to degrade phenolic compounds, etc. problems, to optimize the sewage treatment process, reduce secondary pollution, and improve safety.

Active Publication Date: 2016-01-06
ANHUI QINGMING ENVIRONMENTAL PROTECTION TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, Chinese invention patent, application number 201210539590.6 "A method for degrading octylphenol by laccase" discloses a method for degrading octylphenol by laccase, but the enzyme preparation is expensive, the enzyme reaction conditions are harsh, and the enzyme reaction is low. Defects such as single substance, poor applicability
Chinese invention patent, application number 200910019223.1 "A strain of Pseudomonas XQ23 that efficiently degrades various phenolic compounds" discloses a strain of Pseudomonas that can degrade phenolic compounds, but due to the single enzyme system of a single strain, it cannot degrade phenolic compounds Such compounds are completely degraded and cannot be used for industrial promotion
Chinese invention patent, the patent number is 201210057537.2 "Application of a microbial agent in the biological treatment of phenol-containing wastewater", which discloses an application method of a microbial agent for the treatment of phenol-containing industrial wastewater, but the domestication time of the agent is long , and ignore the safety detection of sludge secondary pollution

Method used

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  • Compound microbial preparation for degrading phenol endocrine disrupter and preparation method
  • Compound microbial preparation for degrading phenol endocrine disrupter and preparation method
  • Compound microbial preparation for degrading phenol endocrine disrupter and preparation method

Examples

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Comparison scheme
Effect test

Embodiment 1

[0056] Example 1: Preparation of compound microbial preparations for degrading phenolic endocrine disruptors

[0057] The preparation method of microbial preparations for degrading phenolic endocrine disruptors according to the present invention, the detailed implementation steps are:

[0058] The first step: seed solution preparation

[0059] One of Klebsiella sp. ACCC11779, ACCC11692, ACCC11624, one of Azospirillum lipoferum ACCC10481, one of Rhodococcus erythropolis ACCC40323, ACCC10214 and Bacillus licheniformis ) ACCC02698, ACCC19372, CGMCC1.10314, and CGMCC1.521 (both strains that have been publicly preserved). After activation on a slant, they are respectively inoculated in triangular flasks containing beef extract peptone liquid medium and cultivated to prepare shake flask seeds. liquid. Shake flask culture conditions are: liquid volume 40%, temperature 33°C, shaker speed 140rpm, culture time 12h;

[0060] The above-mentioned cultivated shake flask seed liquid was r...

Embodiment 2

[0070] Example 2 Degradation of phenolic endocrine disruptor compound microbial preparation preparation

[0071] The detailed steps are:

[0072] The first step: seed solution preparation

[0073] One of Klebsiella sp. ACCC11779, ACCC11692, ACCC11624, one of Azospirillum lipoferum ACCC10481, one of Rhodococcus erythropolis ACCC40323, ACCC10214 and Bacillus licheniformis ) ACCC02698, ACCC19372, CGMCC1.10314, and CGMCC1.521 (all of which are publicly preserved strains), after being activated on a slant, were inoculated in triangular flasks containing beef extract peptone liquid medium, and cultured in shake flasks. Shake flask culture conditions are: liquid volume 38%, temperature 35°C, shaker speed 120rpm, culture time 18h;

[0074] The above-mentioned cultivated shake flask seed liquid was respectively connected to the seed tank for fermentation, the inoculum size was 10%, the culture conditions were: ventilation rate 1:1.2v / v / min, temperature 36°C, stirring speed 150rpm, fe...

Embodiment 3

[0084] Example 3 Degradation of phenolic endocrine disruptor compound microbial preparation preparation

[0085] The preparation process flow of the microbial preparation for degrading phenolic endocrine disruptors according to the present invention: preparation of seed liquid→mixed fermentation→preparation and addition of protective agent→product quality inspection and packaging. The detailed implementation steps are:

[0086] The first step: seed solution preparation

[0087] One of Klebsiella sp. ACCC11779, ACCC11692, ACCC11624, one of Azospirillum lipoferum ACCC10481, one of Rhodococcus erythropolis ACCC40323, ACCC10214 and Bacillus licheniformis ) ACCC02698, ACCC19372, CGMCC1.10314, and CGMCC1.521 (all of which are publicly preserved strains), after being activated on a slant, were inoculated in triangular flasks containing beef extract peptone liquid medium, and cultured in shake flasks. Shake flask culture conditions are: liquid volume 42%, temperature 36°C, shaker sp...

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Abstract

The invention relates to a compound microbial preparation for degrading phenol endocrine disrupter and a preparation method, and relates to microorganism projects and sewage and waste water treatment processes. The compound microbial preparation comprises Klebsiella sp., azospirillum lipoferum, rhodococcuserythropolis and bacillus licheniformis. The microbial content of Klebsiella sp. accounts for 30%-50% of the total microbial content. The microbial content of azospirillum lipoferum accounts for 30%-50% of the total microbial content. The microbial content of rhodococcuserythropolis accounts for 10%-30% of the total microbial content. The microbial content of bacillus licheniformis accounts for 10%-30% of the total microbial content. The compound microbial preparation is fed into an anaerobic tank and an aerobic tank, and the sewage and waste water treatment processes are enhanced. The compound microbial flora can tolerate the high-concentration phenol endocrine disrupter which is used as organic matter needed in growth for being fully utilized and degraded, the sewage and waste water treatment processes are optimized, the secondary pollution of output water and sludge is reduced, and the safety of sludge landfill and agricultural utilization is improved.

Description

technical field [0001] The invention relates to a compound microbial preparation for degrading phenolic endocrine disruptors and a preparation method thereof, relates to microbial engineering and waste water treatment technology, and belongs to the field of environmental protection. Background technique [0002] Endocrine Disrupting Chemicals (EDCs), also known as Environmental Hormone (Environmental Hormone), is an exogenous chemical substance that interferes with the endocrine system. Substances, through ingestion, accumulation and other ways, do not directly bring abnormal effects on organisms as toxic substances, but act on organisms similar to estrogen, even if the amount is very small, they can also make the organisms Endocrine imbalance, various abnormal phenomena appear. Such substances can cause genital disturbances, abnormal behavior, reduced reproductive capacity, larval death, and even extinction in animals and humans. [0003] Phenolic endocrine disruptors in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/30C12R1/01C12R1/22C12R1/10C02F101/34
Inventor 赵海泉汪顺丽谢丽查君茹韩保安张宏才王田野魏荷芬申广勒柴化建赵鸿涛鲍大林胡子全
Owner ANHUI QINGMING ENVIRONMENTAL PROTECTION TECH CO LTD
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