Compound microbial preparation for degrading phenol endocrine disrupter and preparation method
An endocrine disruptor, compound microorganism technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of neglecting the safety detection of secondary pollution of sludge, single enzyme system, and inability to degrade phenolic compounds, etc. problems, to optimize the sewage treatment process, reduce secondary pollution, and improve safety.
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Embodiment 1
[0056] Example 1: Preparation of compound microbial preparations for degrading phenolic endocrine disruptors
[0057] The preparation method of microbial preparations for degrading phenolic endocrine disruptors according to the present invention, the detailed implementation steps are:
[0058] The first step: seed solution preparation
[0059] One of Klebsiella sp. ACCC11779, ACCC11692, ACCC11624, one of Azospirillum lipoferum ACCC10481, one of Rhodococcus erythropolis ACCC40323, ACCC10214 and Bacillus licheniformis ) ACCC02698, ACCC19372, CGMCC1.10314, and CGMCC1.521 (both strains that have been publicly preserved). After activation on a slant, they are respectively inoculated in triangular flasks containing beef extract peptone liquid medium and cultivated to prepare shake flask seeds. liquid. Shake flask culture conditions are: liquid volume 40%, temperature 33°C, shaker speed 140rpm, culture time 12h;
[0060] The above-mentioned cultivated shake flask seed liquid was r...
Embodiment 2
[0070] Example 2 Degradation of phenolic endocrine disruptor compound microbial preparation preparation
[0071] The detailed steps are:
[0072] The first step: seed solution preparation
[0073] One of Klebsiella sp. ACCC11779, ACCC11692, ACCC11624, one of Azospirillum lipoferum ACCC10481, one of Rhodococcus erythropolis ACCC40323, ACCC10214 and Bacillus licheniformis ) ACCC02698, ACCC19372, CGMCC1.10314, and CGMCC1.521 (all of which are publicly preserved strains), after being activated on a slant, were inoculated in triangular flasks containing beef extract peptone liquid medium, and cultured in shake flasks. Shake flask culture conditions are: liquid volume 38%, temperature 35°C, shaker speed 120rpm, culture time 18h;
[0074] The above-mentioned cultivated shake flask seed liquid was respectively connected to the seed tank for fermentation, the inoculum size was 10%, the culture conditions were: ventilation rate 1:1.2v / v / min, temperature 36°C, stirring speed 150rpm, fe...
Embodiment 3
[0084] Example 3 Degradation of phenolic endocrine disruptor compound microbial preparation preparation
[0085] The preparation process flow of the microbial preparation for degrading phenolic endocrine disruptors according to the present invention: preparation of seed liquid→mixed fermentation→preparation and addition of protective agent→product quality inspection and packaging. The detailed implementation steps are:
[0086] The first step: seed solution preparation
[0087] One of Klebsiella sp. ACCC11779, ACCC11692, ACCC11624, one of Azospirillum lipoferum ACCC10481, one of Rhodococcus erythropolis ACCC40323, ACCC10214 and Bacillus licheniformis ) ACCC02698, ACCC19372, CGMCC1.10314, and CGMCC1.521 (all of which are publicly preserved strains), after being activated on a slant, were inoculated in triangular flasks containing beef extract peptone liquid medium, and cultured in shake flasks. Shake flask culture conditions are: liquid volume 42%, temperature 36°C, shaker sp...
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