42results about How to "Suitable for industrial promotion and application" patented technology

The invention relates to an alpha-fetoprotein testing kit (time-resolved fluoroimmunoassay) applicable for mid-pregnancy prenatal screening and a preparation method thereof. The kit comprises the following main components of an experimental buffer solution, a concentrated washing solution, an enhancement solution, a reaction plate, a standard product and a quality control product of filter paper dried blood spots and a europium marker. The method for preparing the kit according to the invention comprises the following steps of: 1. preparing the experimental buffer solution, the concentrated washing solution and the enhancement solution; 2. coating the reaction plate; 3. preparing the standard product and the quality control product; 4. preparing the europium marker; 5. separately loading; 6. sticking labels; and 7. assembling into a finished product. The invention has the characteristics that the accuracy and the sensitivity of a detected result are high, the stability is good, and a detecting method is economical, convenient and safe and has no traumatic property, has high automation degree, and the like. A filter paper dried blood spot technology is applied to prenatal screening work, which is beneficial to blood specimen collection, storage and transportation and ensures the experimental reliability.

The invention discloses a nano vesicle with pH and reduction dual sensitivity as well as a preparation method and application thereof, and belongs to the technical field of high polymer chemistry andbiomedical engineering. The nano vesicle contains an amphiphilic polymer with pH and reduction dual sensitivity, wherein the polymer is composed of a hydrophilic section, polyethylene glycol section and a hydrophobic section, poly(aspartyl (di-n-butyl ethylenediamine-co-mercaptoethylamine)-phenylalanine) section; the ratio of the hydrophilic section to the hydrophobic section is 1:11; the nano vesicle also contains a hydrophilic antitumor drug and a hydrophobic antitumor drug. The nano vesicle provided by the invention can bear hydrophilic and hydrophobic drugs at the same time to realize drugtarget release and high-efficiency cooperative treatment on the tumor part, and can be used in vivo for a long time.

The invention relates to a eucalyptus oil solution for treating animal psoroptic manges and a preparation method thereof, and particularly relates to the eucalyptus oil solution with functions of resisting psoroptic manges of pigs, cows, sheep, rabbits and dogs, and the preparation method thereof. The preparation method comprises the following steps: mixing a stabilizer, a penetrating agent, eucalyptus oil and a solvent, and processing in high-speed mixing and high-pressure homogenization manners. The eucalyptus oil solution has the advantages of being good in preparation stability, high in process repeatability, simple in process, strong in maneuverability, reasonable in cost and the like. The prepared eucalyptus oil solution is colorless and transparent uniform liquid and is convenient to coat or spray, and the defects of low volatility, low adhesive rate and the like in direct use of the eucalyptus oil are avoided. The production practice and veterinary clinical trials show that the eucalyptus oil solution provided by the invention has a significant effect for treatment of the animal psoroptic manges.

The invention relates to the technical field of food processing, and in particular relates to a water chestnut-agaric large meatball and a preparation method thereof. Stuffing of the large meatball is prepared from the following raw materials in parts by weight: 60-80 parts of edible livestock and poultry meat, 0.3-0.4 part of phosphate, 8-10 parts of water chestnuts, 4-6 parts of dry agaric, 0.8-1.0 part of edible salt, 0.8-0.9 part of green onion, 0.5-0.6 part of ginger, 0.7-0.8 part of dark soy sauce, 0.4-0.5 part of sesame oil, 1.5-1.8 parts of starch, 1.5-2.0 parts of breadcrumbs, 0.5-0.8 part of xylitol and 63-84 parts of water. The water chestnuts and agaric provide elastic and brittle tastes, nutritional values of the large meatball are improved, balance of vegetables and meat is realized, and the large meatball has dietary therapy and health effects. Further, the water chestnuts are soaked in a soak solution containing white granulated sugar, edible salt and citric acid to be pretreated when being used, so that browning of the water chestnuts is delayed, the texture of the water chestnuts is changed, and the elastic and brittle tastes of the water chestnuts are enhanced.

The invention discloses a preparation method of a non-fusion anti-desmoglein DSG3 rabbit-derived monoclonal antibody. The preparation method comprises the following steps: 1) selecting a suitable immune animal, and carrying out DSG3 immunization on the animal; 2) extracting successfully immunized animal blood, collecting peripheral blood mononuclear cells, and separating out a B lymphocyte population capable of secreting a DSG3 antibody; 3) establishing a rabbit-derived single-chain antibody yeast display library; 4) constructing a DSG3 rabbit-derived monoclonal antibody expression vector library; 5) constructing a DSG3 rabbit-derived monoclonal antibody yeast expression library: namely converting a pichia pastoris expression vector PZ recombinant plasmid into yeast expression bacteria X33, and screening X33 recombinant transformation monoclonal bacteria capable of secreting a DSG3 antibody; 6) obtaining a X33 recombinant conversion monoclonal strain that can stably secrete a DSG3 antibody; and 7) purifying the obtained DSG3 rabbit-derived monoclonal antibody. The provided preparation method can prepare a stable DSG3 antibody, has industrial repeatability and is suitable for industrial popularization and application.

The invention provides a low-friction-resistance water-based drilling fluid and a preparation method thereof, and relates to the technical field of oilfield chemistry. The low-friction-resistance water-based drilling fluid provided by the invention is prepared from the following preparation raw materials in parts by mass: 75-90 parts of water; 10-25 parts of a polyphosphate aqueous solution; 0.1-2.0 parts of an alkalinity regulator; 1.0-4.0 parts of a filtrate reducer; 0.1-0.6 parts of a rheological modifier; 1.0-3.5 parts of an anti-collapse blocking agent; 1.0-3.5 parts of an inhibitor; and 0-100 parts of barite. The low-friction-resistance water-based drilling fluid provided by the invention abandons the technical thought that an oily or water-based lubricant is added into the conventional water-based drilling fluid, is prepared on the basis of a polyphosphate aqueous solution with super-strong lubricity, and has the lubricating property comparable with that of an oil-based drilling fluid. The problems of friction resistance and torque of extended reach wells and horizontal wells can be solved.

The invention relates to a turmeric rhizome composite solid preparation which is prepared by taking medicinal and edible resource of turmeric rhizome extracts including curcumin, turmeric volatile oiland turmeric soluble fibers as raw materials. The turmeric rhizome composite solid preparation is composed of the following components in percentage by weight: 0.5%-30% of the curcumin, 0.5%-50% of the turmeric soluble fibers, 1%-10% of the turmeric volatile oil, 0.5%-10% of a food emulsifier and the balance of a filling agent. According to the technology disclosed by the invention, effective components of the curcumin, the turmeric volatile oil and the turmeric soluble fibers of turmeric rhizomes are fully extracted and applied, the turmeric rhizome composite solid preparation is obtained through emulsion homogenizing emulsification, concentration and drying processes, the water solubility and bioavailability of the curcumin are effectively improved, and the turmeric rhizome composite solid preparation can be used as a functional raw material to be applied to the fields of foods, nutritional health-care products, medicines and the like.

The invention discloses a braze welding packaging and manufacturing method for making a fiber bragg grating into a double-peak grating. The braze welding packaging and manufacturing method comprises the following steps of: taking one fiber bragg grating at will, wherein a grating forming part of the grating is a naked grating, and the surface is free from any protective layer; carrying out surface metallization to the fiber bragg grating, wherein the metallization thickness is 30-50 micrometers; corroding a half of a surface metallization layer along the grating length direction by nitric acid, and reserving the other half; putting the grating part with the metallization layer into an aluminum alloy tube; guaranteeing that the gap is between 30 and 50 micrometers; putting low-temperature brazing paste between the grating and the inner wall of the aluminum alloy tube, wherein the brazing paste can be medium-and-low-temperature Sn base or Al base brazing paste; putting the aluminum alloy tube and the built-in grating into a temperature-control furnace; heating to be 30-50DEG C above a brazing material melting point; and keeping warm for 20-50 seconds, thus obtaining a double-wavelength fiber bragg grating after slowly cooling. The braze welding packaging and manufacturing method disclosed by the invention has a simple technical process, and the obtained double-peak grating can be used for the field that a plurality of physical quantities are simultaneously subjected to discriminating measurement.

The invention provides a lithium iron phosphate water-based positive electrode slurry, a preparation method thereof and an environment-friendly long-circulation type lithium ion battery. Solid powder lithium iron phosphate, CMC and conductive carbon black are firstly subjected to dry mixing, then deionized water, EC and a PAA solution are added, kneading is performed, then deionized water and CNTs are added, high-rotating-speed dispersion is performed, after the dispersion is finished, an SBR emulsion is added, and mixing is performed to obtain uniform and stable water-based positive electrode slurry; and the water-based positive electrode slurry can be used for preparing the environment-friendly long-circulation type lithium ion battery through the effects of coating and the like. According to the invention, deionized water is adopted as the solvent of the positive electrode slurry, so that the solvent is environment-friendly, secondary recovery is not needed, the source is rich, and the preparation cost is low; and the 25 DEG C cycle performance of a prepared water-based lithium iron phosphate battery cell is as follows: the capacity retention rate reaches about 95% after 1000 cycles. Moreover, the lithium ion battery provided by the invention is good in processing stability.

A method for preparing copper powder and ferrous sulfate by using a solution leached through a sludge containing copper wet method comprises the following steps that firstly, the solution is leached through the sludge containing copper wet method and filtered; the filtered leaching agent is transferred into a reactor, iron powder is added for a replacement reaction, the concentration of Cu2+ in the leaching agent is reduced to be less than 0.5 g / L after the reaction is complete, solid-liquid separation is conducted, and the copper powder and the replaced solution are obtained; a reducing agent is added to the replaced solution, and the redox potential in the replaced solution is controlled to be reduced to be less than 400 mV; the replaced solution is transferred to an impurity removal reaction tank, heating is conducted, an inorganic solution containing phosphate radical is slowly added, solid-liquid separation is conducted, and phosphate precipitate and impurity-removed liquid are obtained; and the concentration of Fe2+ ions in the impurity-removed liquid is increased to be greater than 300 g / L after evaporation and concentration, transferring to a cooling system is conducted for crystallization, and a ferrous sulfate heptahydrate product is obtained after washing. By the adoption of the method, the cost is low, environmental protection is achieved, the added value of products is high, and resource utilization of waste resources can be achieved.

The invention provides a nematicidal candida and its preparation method and application, belonging to the technical field of microbial pesticides. The bacterial strain of the present invention is Candida krusei (Candida krusei) TY9, which was preserved in the China Center for Type Culture Collection on September 20, 2014, and the preservation number is CCTCC M 2014435. The Candida krusei The TY9 strain is prepared by conventional liquid fermentation culture, and is used in the prevention and treatment of plant parasitic nematodes, especially has a good control effect on all-tooth resurrection nematodes and / or pine wood nematodes, and has low cost, simple preparation process, easy-to-obtain raw materials, and nematodecidal The invention has the advantages of good effect and the like, and is suitable for industrial promotion and application.

The invention discloses a high-sensitivity real-time fluorescence detection method. The invention provides a method for detecting a target nucleic acid in a detected sample, which comprises: mixing the sample with a polymerase chain reaction(PCR) amplification agent and a fluorescence labeling probe, wherein the nucleic acid part of the fluorescence labeling probe comprises a first complementary sequence, a target nucleic acid specific sequence, a second complementary sequence, and an extension sequence, and the first complementary sequence and the second complementary sequence can complementand match with each other; and performing PCR and real-time fluorescence detection. The invention also provides a detection kit, use of the detection kit in preparation of a detection product, and primers, probes and other reagents used in the detection kit.

The invention discloses an antimony sulfide-based composite material and a preparation method and application thereof. The antimony sulfide-based composite material is prepared from natural stibnite and carbon materials. The suspension of the carbon material is mixed and then subjected to hydrothermal treatment and calcination to obtain the antimony sulfide-based composite material. The antimony sulfide-based composite material provided by the invention uses natural stibnite as raw material, avoids the problem of environmental pollution and high cost in the process of preparing high-purity antimony sulfide, and has a simple and efficient technological process, greatly reduces the preparation cost, and is stable and reliable. , the obtained antimony sulfide-based composite material has a wide range of applications, and has broad application prospects as a negative electrode material for lithium ion or sodium ion batteries.