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A kind of candida with nematicide activity and its preparation method and application

A Candida, nematicide technology, applied in the direction of nematicides, botany equipment and methods, microbe-based methods, etc., can solve limited problems, achieve wide applicability, low production cost, and good nematicide activity Effect

Active Publication Date: 2017-02-15
NANYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are limited technical reports on the biological control of nematodes by Candida, and there are no commercial biological control agents derived from Candida for the control of nematodes on the market, which is still a technical blank

Method used

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  • A kind of candida with nematicide activity and its preparation method and application
  • A kind of candida with nematicide activity and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The method for preparing the Candida krusei TY9 strain is first prepared through test tube culture, and then through liquid expansion culture, which can be carried out as follows:

[0029] ① Test tube culture

[0030] The weight ratio of the medium is: 3% yeast extract, 2% peptone, 4% glucose, 3% sodium chloride, 2% agar, pH6.5; the Candida krusei TY9 cells were inoculated On the above-mentioned medium, culture at 30°C for 3 days to obtain test-tube species;

[0031] ②Liquid expansion culture

[0032]Liquid culture medium is made up of the raw material of following percentage by weight: 3% yeast extract, 2% peptone, 4% glucose, 3% sodium chloride, surplus is water, pH6.5; In 500mL Erlenmeyer flask liquid medium, each bottle was filled with 200mL, and cultured on a shaker at 30°C for 5 days at a speed of 300r / min.

[0033] The extraction method of the nematocidal active ingredient in the fermented liquid obtained by the expansion of the above-mentioned liquid is as fo...

Embodiment 2

[0035] The method for preparing the Candida krusei TY9 strain is first prepared through test tube culture, and then through liquid expansion culture, which can be carried out as follows:

[0036] ① Test tube culture

[0037] The weight ratio of the medium is: 2% yeast extract, 1.2% peptone, 2.5% glucose, 2% sodium chloride, 1.9% agar, pH6; the Candida krusei TY9 cell is inoculated into the above-mentioned culture medium at 28°C for 2 days to obtain test tube seeds;

[0038] ②Liquid expansion culture

[0039] The liquid medium is composed of the following raw materials in weight percentage: 2% yeast extract, 1.2% peptone, 2.5% glucose, 2% sodium chloride, the balance is water, pH6; inoculate the test tube prepared in step ① into a 500mL triangular Bottles of liquid culture medium, 200mL per bottle, cultured on a shaker at 26°C for 3 days at a rotational speed of 250r / min.

[0040] The extraction method of the nematicidal active ingredient in the fermented liquid obtained by ...

Embodiment 3

[0042] The method for preparing the Candida krusei TY9 strain is first prepared through test tube culture, and then through liquid expansion culture, which can be carried out as follows:

[0043] ① Test tube culture

[0044] The weight ratio of the medium is: 1% yeast extract, 0.5% peptone, 1% glucose, 1% sodium chloride, 1.8% agar, pH5; the Candida krusei TY9 cell is inoculated into the above-mentioned culture medium at 24°C for 1 day to obtain test tube species;

[0045] ②Liquid expansion culture

[0046] The liquid medium is composed of the following raw materials in weight percentage: 1% yeast extract, 0.5% peptone, 1% glucose, 1% sodium chloride, the balance is water, pH5; inoculate the test tube prepared in step ① into a 500mL triangular Bottles of liquid culture medium, 200mL per bottle, cultured on a shaker at 24°C for 2 days at a rotational speed of 200r / min.

[0047] The extraction method of the nematicidal active ingredient in the fermented liquid obtained by the...

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Abstract

The invention provides a nematicidal candida and its preparation method and application, belonging to the technical field of microbial pesticides. The bacterial strain of the present invention is Candida krusei (Candida krusei) TY9, which was preserved in the China Center for Type Culture Collection on September 20, 2014, and the preservation number is CCTCC M 2014435. The Candida krusei The TY9 strain is prepared by conventional liquid fermentation culture, and is used in the prevention and treatment of plant parasitic nematodes, especially has a good control effect on all-tooth resurrection nematodes and / or pine wood nematodes, and has low cost, simple preparation process, easy-to-obtain raw materials, and nematodecidal The invention has the advantages of good effect and the like, and is suitable for industrial promotion and application.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a candida with nematicidal activity and a preparation method and application thereof. Background technique [0002] As an important class of pathogens, nematodes have become an important problem in agricultural and forestry production. There are more than 200 genera and 5,000 species of plant parasitic nematodes reported in the world. Crop pathogenic nematodes harm more than 3,000 plants such as grain, oilseeds, vegetables, fruits, cotton, soybeans, peanuts, Panax notoginseng, and American ginseng. become the second largest disease in agricultural production. According to incomplete estimates, plant parasitic nematodes cause global losses of US$ 157 billion per year. Bine wood nematodes, root-knot nematodes, and cyst nematodes are among the most serious nematodes that attack many important crops. Pine wood nematode is a devastating disease of pine tre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16A01N63/04A01P5/00C12R1/72
CPCA01N63/30C12N1/165C12R2001/72
Inventor 牛秋红张林丁锋唐悦和晶亮曹锋张果
Owner NANYANG NORMAL UNIV
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