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Nematophagous fungus and preparation method and application thereof

A technology for nematophagous fungi and fungi, which is applied to nematophagous fungi and the preparation method and application fields thereof, can solve the problems of strong lethality of beneficial organisms, environmental pollution, unsafe human and livestock, etc., and achieve the effect of good nematicidal activity

Inactive Publication Date: 2012-08-22
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to people's long-term overreliance on chemical pesticides and lack of ecological awareness, serious environmental pollution has been caused
As far as single nematicides are concerned, most of the highly toxic chemical pesticides used in the past are unsafe for humans and animals, seriously pollute the environment, and easily cause nematodes to develop resistance, and are highly lethal to beneficial organisms.
At the same time, with the enhancement of people's awareness of environmental protection and the increasing demand for healthy food, the highly toxic pesticides used to control plant nematodes in the past are mostly banned in the form of laws.

Method used

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  • Nematophagous fungus and preparation method and application thereof
  • Nematophagous fungus and preparation method and application thereof
  • Nematophagous fungus and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1 Dactylella hainanensis lg21-1 bacteria test tube culture

[0058] The medium formula is potato agar medium (PDA): 200g potato, 20g glucose, 20g agar powder, 1000ml water.

[0059] Dactylella hainanensis lg21-1 mycelium is inoculated on the slant of PDA medium, cultured at 20-30° C. for 6-12 days to obtain test tube species.

Embodiment 2

[0060] The solid culture of embodiment 2lg21-1 bacterium

[0061] Inoculate the preserved bacteria of Dactylella hainanensis lg21-1 into CMA medium (composition: 20g corn flour, 15g agar powder, 1000ml water) to activate, then inoculate into YMA medium (composition: 2g yeast extract powder , 20g maltose, 1000ml water) (petri dish with a diameter of 75mm), cultured in dark at 25°C for 16d. Store the bacteria in a 4°C refrigerator for later use, or directly prepare the spore culture solution for use.

Embodiment 3

[0062] The solid culture of embodiment 3lg21-1 bacterium

[0063] Inoculate the preserved bacteria of Dactylella hainanensis lg21-1 into CMA (composition: 20g corn flour, 15g agar powder, 1000ml water) medium for activation, and then inoculate into the medium of the following formula: 1g KH 2 PO 4 , 0.5g MgSO 4 , 0.5g NaCl, 0.05g ZnSO 4 , 0.05g FeSO 4 , 15g agar powder, 20g glucose, add 1000ml ultrapure water, adjust the pH value to 6.5-7.0. Others are the same as embodiment 2.

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Abstract

The invention discloses a nematophagous fungus, and a preparation method and the application thereof. The nematophagous fungus is dactylella hainanensis lg21-1 of a new species of dactylella and is preserved at the China Center for Type Culture Collection on 4th February in 2010 with a preservation number of CCTCC No.: M 2010042. The invention provides the preparation method for the nematophagousfungus, which comprises a test-tube culture method, a liquid culture method or a solid culture method. The fungus of the invention has the prominent advantage of high virulence to nematodes, applicability to the preparation of bio-control agents for plant parasitic nematodes and relatively vaster application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a nematode fungus and its preparation method and application. Background technique [0002] Plant parasitic nematodes are an important class of pathogenic microorganisms with wide distribution and many species. There are more than 200 genera and 5,000 species of plant nematodes reported in the world, causing serious damage to agricultural and forestry production (Feng Zhixin, 2001). Worldwide annual crop losses from plant-parasitic nematodes are estimated to be US$ 78 billion (Barker et al, 1998). As far as the nematode species that is most harmful today—root-knot nematode, it has a wide range of hosts and is distributed worldwide, which can cause more than 10% loss of global crop production (Whitehead, 1998). [0003] Plant nematodes have the characteristics of concealment, multi-host, stubbornness, and easy transmission. Their population grows rapidly,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P5/00C12R1/645
Inventor 廖金铃李玉中卓侃罗梅
Owner SOUTH CHINA AGRI UNIV
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