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Trichoderma fungus with nematicide activity as well as preparation method and application thereof

A Trichoderma, nematicide technology, applied in the direction of nematicides, botany equipment and methods, microbe-based methods, etc., can solve limited problems and achieve good effect of nematicidal activity

Active Publication Date: 2013-05-01
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, relevant technical reports on Trichoderma viridans are limited, and there are no commercial biocontrol agents derived from Trichoderma viridans, especially Trichoderma viridans, for the control of nematodes on the market, which is still a technical blank

Method used

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  • Trichoderma fungus with nematicide activity as well as preparation method and application thereof
  • Trichoderma fungus with nematicide activity as well as preparation method and application thereof
  • Trichoderma fungus with nematicide activity as well as preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0032] The isolation and identification of embodiment 1 Trichoderma viridans (H.virens H22)

[0033] The medium formula is CMA medium: 20g corn flour, 15g agar powder, 1000ml water.

[0034] Sprinkle 1.0g soil sample (taken from the mangrove rhizosphere in Shenzhen Bay, Guangdong Province) on the culture medium, insert about 1000 second-instar larvae (J2) of Meloidogyne incognita, culture them at 25°C for 3 days, examine them under the microscope and pick them up with a needle Single fungal conidia on and around the dead insect body were placed on a CMA plate containing 50 ppm of streptomycin sulfate, and the fungus was purified.

[0035] The bacterial strain morphological characteristic of described Trichoderma viridans (Hypocrea virens H22) is: initial stage is white on PDA, and mid-term color becomes light green gradually, has well-developed aerial hyphae, and later stage produces a large amount of fractional sporophytes and conidia , conidiophores are opposite or alternat...

Embodiment 2

[0058] The solid culture of embodiment 2 Trichoderma viridans (H.virens H22)

[0059] The medium formula is potato agar medium (PDA medium): 200g potato, 20g glucose, 20g agar powder, 1000mL water;

[0060] In each 90mm petri dish, pour about the thick PDA medium that accounts for the height of 1 / 3 of the petri dish, Trichoderma viridans (H.virens H22) mycelium is inoculated on the PDA medium, after sealing with parafilm Cultivate at 25-28°C for 2-3 days. Store the strains in a refrigerator at 4°C until use, or use them directly with spore culture solution.

Embodiment 3

[0061] The liquid culture of embodiment 3 Trichoderma viridans (H.virens H22)

[0062] The medium formula is CMA culture medium: corn flour 20g, distilled water 1000mL.

[0063] Each 500mL triangular flask was filled with 200mL of CMA culture solution, sterilized by damp heat at 121°C for 20min, and after cooling, 5 pieces of activated Trichoderma viridans discs were inserted into each bottle, and cultured in the dark at 180rmp 27°C for 8 days. Centrifuge at 12000rmp for 10min to obtain a supernatant, which is stored in a refrigerator at 4°C until use.

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Abstract

The invention discloses a trichoderma fungus with nematicide activity as well as a preparation method and an application thereof. The trichoderma fungus with nematicide activity is Hypocrea virens H22 which is preserved in China Center for Type Culture Collection on September 2nd, 2011, and the preservation number is CCTCC M 2011303. The preparation method of the nematicide fungus provided by theinvention comprises a liquid culture method or a solid culture method. The strains have good nematicide activity on the nematode, can be applied to the preparation of plant parasitic nematode biocontrol agents and have better application prospects.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a nematocidal fungus and a preparation method and application thereof. Background technique [0002] Plant parasitic nematodes are an important class of pathogenic microorganisms with wide distribution and many species. There are more than 200 genera and 5,000 species of plant nematodes reported in the world, causing serious damage to agricultural and forestry production (Feng Zhixin, 2001). Worldwide annual crop losses from plant-parasitic nematodes are estimated to be US$ 78 billion (Barker et al, 1998). As far as the nematode species that is the most harmful today, root-knot nematode, has a wide range of hosts and is distributed worldwide, it can cause more than 10% loss of global crop production every year (Whitehead, 1998). [0003] Plant nematodes have the characteristics of concealment, multi-host, stubbornness, and easy transmission, and their po...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P5/00C12R1/645
Inventor 廖金铃卓侃唐照磊
Owner SOUTH CHINA AGRI UNIV
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