The invention discloses a honeybee pupae wine which is made of the following raw materials in parts by weight: 100 parts of rice wine, 20-30 parts of wild honeybee pupae, 5-10 parts of Angelica sinensis, 2-5 parts of ginseng, 10-15 parts of radix astragali, 1-5 parts of pilose antler, 1-5 parts of epimediums, 5-10 parts of uncaria, 5-9 parts of Carthamus tinctorius L., 1-5 parts of liquorice, 5-10 parts of rhizoma chuanxiong, 10-15 parts of wild rhizoma gastrodiae, 3-6 parts of bighead atractylodes rhizome, 6-10 parts of wolfberries, 3-8 parts of donkey-hide gelatin, 2-7 parts of suberect spatholobus stems and 1-5 parts of honeybees. The preparation method of the honeybee pupae wine is simple and practicable; and the prepared honeybee pupae wine is a beverage wine made according to the production and processing processes with the rice wine being as the wine base, and the edible animals and plants ( the wild honeybee pupae, the Angelica sinensis, ginseng, the radix astragali, the pilose antler, the epimediums, the uncaria, the Carthamus tinctorius L., the liquorice, rhizoma chuanxiong, the wild rhizoma gastrodiae, the bighead atractylodes rhizome, the wolfberries, the donkey-hide gelatin, the suberect spatholobus stems and the honeybees) being as aroma and flavor-producing substances. The honeybee pupae wine utilizes the nourishing and tonic effect and weight reducing and qi-tonifying effect of the honeybee pupae, combines Chinese patent medicines which enrich and benefit essence and blood and strengthen tendons and bones, and adopts the ultrasonic wave aging accelerating technology, so that the wine has the advantages of presenting a deep amber color, being clear and transparent, being soft and sweet in taste, being not violent and pungent and being long in aftertaste; and the wine has notable effects.