The invention relates to an impact-resistant epoxy vinyl ester resin and its preparation method and use. The impact-resistant epoxy vinyl ester resin comprises, by mass, A, 40-50% of epoxy resin, B, 5-25% of unsaturated monocarboxylic acid, C, 0.03-0.1% of a polymerization inhibitor, D, 0.2-0.5% of a catalyst and E, 30-40% of a crosslinking monomer. The epoxy resin is one of bisphenol F epoxy resin, hydrogenated bisphenol A epoxy resin, and dimer acid-modified epoxy resin. The preparation method utilizes a ring-opening esterification reaction of the flexible epoxy resin and organic unsaturated monocarboxylic acid, utilizes an appropriate raw material ratio, greatly improves resin toughness, realizes resin casting impact strength of 20-30KJ/m<2>, utilizes less types of the raw materials, has simple synthesis processes, is environmentally friendly, and does not produce three wastes. The impact-resistant epoxy vinyl ester resin has good stability.