Method for selectively producing 3-hydroxyl-2-butanone and 2, 3-butanediol through microbial fermentation

A selective, butanediol technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as non-compliance with industrial safety production, achieve high industrial application value, and reduce costs.

Active Publication Date: 2014-01-22
JIANGNAN UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

In addition, these strains are potentially pathogenic and do

Method used

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  • Method for selectively producing 3-hydroxyl-2-butanone and 2, 3-butanediol through microbial fermentation
  • Method for selectively producing 3-hydroxyl-2-butanone and 2, 3-butanediol through microbial fermentation
  • Method for selectively producing 3-hydroxyl-2-butanone and 2, 3-butanediol through microbial fermentation

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Embodiment 1

[0023] (1) B.amyloliquefaciens CCTCC M2012349 seed culture

[0024] Pick a single colony from the activated plate and inoculate it in the seed medium for seed culture. The culture temperature is 37°C, the shaker speed is 160r / min, and the culture time is about 12h. The composition of the seed medium: yeast extract 5g / L, tryptone 10g / L, NaCl10g / L;

[0025] (2) B.amyloliquefaciens CCTCC M2012349 fermentation culture

[0026] The initial fermentation culture volume is 2.5L, and the components of the fermentation medium used are as follows:

[0027] 3-Hydroxy-2-butanone fermentation medium composition: glucose 160g / L, corn steep liquor 5g / L, urea 3g / L, sodium citrate 6g / L, K 2 HPO 4 4g / L, MgSO 4 0.2g / L; adjust the pH of the above fermentation medium to 6.5 with 5mol / L NaOH, and sterilize at 121°C for 30min.

[0028] 3-Hydroxy-2-butanone fermentation Fermentation conditions: Inoculate the above-mentioned cultured seed liquid into the fermentation medium with a 4% inoculation a...

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Abstract

The invention discloses a method for selectively producing 3-hydroxyl-2-butanone and 2, 3-butanediol by using an environmental safety bacterial strain Bacillus amyloliquefaciens with the CCTCC NO. M2012349 and changing the content of corn steep liquor in a medium, the stirring speed and the ventilation capacity, and belongs to the field of a fermentation technology in biological engineering. The method is characterized in that when the 3-hydroxyl-2-butanone is mainly synthesized, the concentration of the corn steep liquor in the fermentation medium can be reduced to be more than or equal to 5g/L, and the relatively high dissolved oxygen level (the air mass flow is 240m<3>/h.m<3> medium, and the stirring rotating speed is 450r/min) is maintained in a fermentation process; and when the 2, 3-butanediol is synthesized, the concentration of the corn steep liquor in the fermentation medium can be increased to be more than or equal to 40g/l, the relatively low dissolved oxygen level (the air mass flow is 120m<3>/h.m<3> medium, and the stirring rotating speed is 350r/min) is maintained in a fermentation process.

Description

technical field [0001] The invention relates to the selective production of acetoin and 2,3-butanediol by using a safe bacterial strain to ferment glucose, which belongs to the technical field of fermentation in bioengineering. Background technique [0002] With the increasing shortage of energy resources and the increasingly serious environmental problems, the development of renewable biomass resources as raw materials, the biorefining technology of producing various chemicals, functional materials and energy substances through chemical, biological and integrated methods is becoming more and more important. more and more attention at home and abroad. 2,3-Butanediol is an important chemical raw material and liquid fuel, widely used in chemical industry, food, fuel, aerospace and other fields. 2,3-butanediol is dehydrogenated to form diacetyl, which is a high-value food flavoring agent with certain antibacterial effect. 2,3-butanediol is a very valuable liquid fuel, and its...

Claims

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Application Information

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IPC IPC(8): C12P7/26C12P7/18C12R1/07
Inventor 饶志明杨套伟张显徐美娟
Owner JIANGNAN UNIV
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