Prawn low dissolved oxygen resistance family selection method
A low-dissolved oxygen, prawn technology, applied in fish farming, application, climate change adaptation, etc., can solve the problems of low survival rate of aquaculture, slow growth of prawns, etc., and achieve clear parental genetic background, convenient management, and reduce maintenance costs Effect
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Embodiment 1
[0022] A method for breeding prawns resistant to low dissolved oxygen families, comprising the steps of:
[0023] (1) Establishment of the basic population: collect Zhongxing No. 1, ZHONGXING No. Zhongke No. 1 and Guihai No. 1 broodstock were used as groups, and 15 mating combinations were established through group mating as the basic group for breeding;
[0024] (2) Construct the F1 generation family: From the above 15 mating combinations, select the individual with a 2% selection intensity, select a large individual, 45g of female shrimp, 35g of male shrimp, 300 female shrimp, 100 male shrimp, and male and female shrimp gonads With normal development, complete appendages, no disease, strong appetite, and sensitive to external stimuli, they will be kept as broodstock. Through artificial insemination, 60 full-sib families and male half-sib families will be established according to the ratio of male to female at 2:1. 30, for the F1 generation family;
[0025] (3) Cultivation ...
Embodiment 2
[0031] A method for breeding prawns resistant to low dissolved oxygen families, comprising the steps of:
[0032] (1) Establishment of the basic population: collect Zhongxing No. 1, ZHONGXING No. Zhongke No. 1 and Guihai No. 1 broodstock were used as groups, and AFLP technology was used to analyze the genetic diversity of broodstock from different sources, and 15 mating combinations were established through group mating as the basic population for breeding;
[0033] (2) Construct the F1 generation family: From the above 15 mating combinations, select the individual with a 2% selection intensity, select the individual with 65g female shrimp and 55g male shrimp, 400 female shrimp, 200 male shrimp, and the gonads of male and female shrimp Normal development, complete appendages, no disease, strong appetite, and sensitive to external stimuli, as broodstock shrimp, through artificial insemination, according to the ratio of male to female at 2:1, to establish 100 full-sib families a...
Embodiment 3
[0039] Example 3 A method for breeding prawns resistant to low dissolved oxygen families, including the following steps: (1) Establishment of basic populations: Collected from SIS of Florida Shrimp Improvement Company in the United States, Konna Bay of Kona Bay Marine Resources Company, Hawaii Ocean Research Institute OI, Thailand Zhengda Broodstock Company ZD and domestically selected parent shrimps Zhongxing No. 1, Zhongke No. 1, and Guihai No. 1 are used as a group, and 15 mating combinations are established through group mating as the basic group of breeding; (2) Construct the F1 generation family: From the above 15 mating combinations, select the individual with a 2% selection intensity, with 55g female shrimp and 45g male shrimp, 400 female shrimp and 150 male shrimp, and the gonad development of male and female shrimp is normal. With complete appendages, no disease, strong appetite, and sensitive to external stimuli, they were kept as broodstock. Through artificial insem...
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