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Screening method of denitrifying bacteria

A screening method, a technology of denitrification bacteria, applied in the field of environmental microorganisms, can solve the problems of no domestication process, restrictions, etc., and achieve the effect of good performance, strong adaptability, and good tolerance

Active Publication Date: 2012-05-09
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN101434905A describes a method for isolating and screening heterotrophic nitrifying bacteria. The heterotrophic nitrifying bacteria obtained by the method are separated from beef extract peptone medium, and have not been domesticated by other carbon sources, so they are limited in the treatment of different sewage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Enrichment culture: Take a certain amount of activated sludge from the aerobic aeration tank of the sewage treatment plant of Zhenhai Refinery of Sinopec, and put it into the aerobic reactor. )2.5mg·L - 1 Under certain conditions, the method of gradually increasing the concentration of ammonia nitrogen in the matrix is ​​used for enrichment. The initial concentration of ammonia nitrogen in the sewage is 100mg / L, and the concentration of COD is 150mg / L. Increase the ammonia nitrogen concentration by 100mg / L from the previous time until it reaches 1000mg / L. Then take a certain amount of enriched sludge and put it into the facultative reactor, at a temperature of 25°C, pH 7.5, DO (dissolved oxygen) 0.1mg·L -1 The culture of heterotrophic bacteria was carried out under the condition that the COD concentration in the culture solution was 1800mg / L, and the ammonia nitrogen concentration was 800mg / L. Cultivate until the ammonia nitrogen concentration is lower than 150mg...

Embodiment 2

[0031] (1) Enrichment culture: Take a certain amount of activated sludge from the aerobic aeration tank of the sewage treatment plant of Fushun Catalyst Plant of Sinopec Catalyst Company, and put it into the aerobic reactor. Dissolved oxygen) 5mg·L -1 Under these conditions, enrichment is carried out by gradually increasing the concentration of ammonia nitrogen in the substrate. The initial concentration of ammonia nitrogen in the sewage is 200mg / L, and the concentration of COD is 200mg / L. The last increase was 150mg / L until it was raised to 1500mg / L. Then take a certain amount of enriched sludge and put it into the facultative oxygen reactor, at a temperature of 30°C, pH 7.8, DO (dissolved oxygen) 0.15mg·L -1 The culture of heterotrophic bacteria was carried out under the condition that the COD concentration in the culture solution was 2500mg / L, and the ammonia nitrogen concentration was 1000mg / L. Cultivate until the ammonia nitrogen concentration is lower than 150mg / L.

...

Embodiment 3

[0036] A certain amount of activated sludge was taken from the aerobic aeration tank of the refinery sewage treatment plant of Sinopec Guangzhou Petrochemical Company. The access temperature: 28°C, pH: 8.0, DO: 4mg·L -1 The enrichment is carried out in the aerobic reactor. The initial ammonia nitrogen concentration of the sewage is 150mg / L, and the COD concentration is 300mg / L. L until it is increased to 1200mg / L. Then take a certain amount of enriched sludge and put it into the facultative oxygen reactor, at a temperature of 28°C, pH 7.8, DO (dissolved oxygen) 0.2mg·L -1 The culture of heterotrophic bacteria was carried out under the condition that the COD concentration in the culture solution was 2500mg / L, and the ammonia nitrogen concentration was 1200mg / L. Cultivate until the ammonia nitrogen concentration is lower than 150mg / L.

[0037] Take a certain amount of the above-mentioned mud-water mixture and dilute it in different times and spread it on a solid medium plate f...

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Abstract

The invention discloses a screening method of denitrifying bacteria, which comprises the processes that: (1) the denitrifying bacterium group enrichment culture is carried out on aerobic activated sludge by ammonia-containing sewage; (2) the heterotrophic bacterium group culture is carried out under the condition of low dissolved oxygen; (3) heterotrophic nitrobacteria are separated and purified from the enriched heterotrophic bacterium group; (4) the purified heterotrophic nitrobacteria are subjected to growing capacity domestication; and (5) the denitrification capacity domestication is carried out on the purified thalli by the ammonia-containing sewage. The denitrifying bacteria screened by the method are applied to the sewage denitrifying treatment, the defects of the traditional biological denitrification technology can be overcome, the denitrifying bacteria can also be used as bioremediation agents to be replenished into an impacted sewage treatment system under different environment conditions, the processing capability of the system is fast restored, the nitration ammonia nitrogen removal capability is realized, simultaneously, the denitrification total nitrogen removal capability is also realized, and conditions are prepared for simultaneous nitrification and denitrification nitrogen removal.

Description

technical field [0001] The invention belongs to the technical field of environmental microbes, and in particular relates to a screening method for denitrifying bacteria with both nitrification biological activity and denitrification ability. Background technique [0002] The traditional biological nitrogen removal process is mostly carried out by two types of bacteria in two different reactors, resulting in a large system footprint and weak impact resistance. Simultaneous nitrification and denitrification (SND) denitrification can simultaneously perform nitrification and denitrification to remove nitrogen and total nitrogen in the same reactor. This new biological denitrification process not only overcomes some problems existing in the traditional biological denitrification process, but also reduces It has outstanding advantages in terms of energy consumption and material consumption: it can effectively maintain the pH stability in the reactor, reduce or cancel the addition ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/12
CPCY02W10/10
Inventor 高会杰黎元生李志瑞许谦刘忠生
Owner CHINA PETROLEUM & CHEM CORP
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