Incubation method of artemia and breeding method of macrobrachium rosenbergii

Abstract

The application relates to a method for hatching artemia and a method for breeding macrobrachium rosenbergii. The method for hatching artemia comprises the following steps: soaking and disinfecting artemia eggs in a first disinfectant, cleaning the artemia eggs with water, and then hatching the artemia eggs in salt water to obtain artemia nauplii; soaking and disinfecting the artemia nauplii in a second disinfectant, cleaning the artemia nauplii with water, and then hatching the artemia nauplii in seawater containing a composite beneficial microorganism preparation to obtain artemia nauplii carrying the composite beneficial microorganism; and the composite beneficial microorganism preparation comprises lactic acid bacteria and butyric acid bacteria. The method for breeding macrobrachium rosenbergii comprises the following steps: arranging macrobrachium rosenbergii larvae in a breeding water body, feeding the artemia nauplii carrying the composite beneficial microorganism on the second day of the arrangement, and increasing the feeding amount day by day until the macrobrachium rosenbergii larvae develop to the eighth stage, and then mainly feeding egg custard and supplementarily feeding the artemia nauplii carrying the composite beneficial microorganism. The application solves the problem of low metamorphosis development rate of the larvae in the prior art method for breeding macrobrachium rosenbergii.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of aquaculture, in particular to a method for hatching brine shrimp and a method for breeding Macrobrachium rosenbergii. BACKGROUND

[0002] In recent years, Macrobrachium rosenbergii has encountered a problem in the process of breeding: when the larvae develop to Z11 (breeding for 15-16 days), a large number of larvae die on the spot. These larvae show symptoms of reduced feeding desire, low metamorphosis development rate and a large number of bottom sinking. Laboratory test results show that most of the sick larvae have empty stomachs and empty intestines. Transcriptome analysis further indicates that the content of beneficial microorganisms in the intestines of the larvae is low.

[0003] Since the Macrobrachium rosenbergii larvae are too small to directly feed on granular feed, they mainly rely on brine shrimp nauplii for food in the early stage of cultivation. After hatching for 12 days, the larvae can gradually adapt to and feed on part of the egg cake feed (which needs to be sterilized at high temperature before each feeding). The traditional bait feeding method is not effective in solving the problem of intestinal health, and cannot solve the root problem. Therefore, it is urgent to find a more effective method to improve the immunity and feeding capacity of Macrobrachium rosenbergii larvae, promote their normal metamorphosis development process, and thus improve the overall success rate of breeding. This is not only a major challenge to the aquaculture industry, but also an important part of ensuring the sustainable development of aquatic resources. SUMMARY

[0004] In view of the shortcomings of the existing breeding technology, the purpose of the present application is to provide a method for hatching brine shrimp and a method for breeding Macrobrachium rosenbergii, so as to solve the problem of low metamorphosis development rate of larvae in the existing breeding method of Macrobrachium rosenbergii.

[0005] In order to achieve the above-mentioned purpose, the technical scheme adopted by the present application is as follows:

[0006] A method for hatching brine shrimp, comprising the following steps:

[0007] S1, soaking the brine shrimp eggs in a first disinfectant for a first disinfection time, then washing with water and placing in a hatching brine with a salinity of 15‰-18‰ for hatching, to obtain brine shrimp nauplii;

[0008] S2, soaking the brine shrimp nauplii in a second disinfectant for a second disinfection time, then washing with water and placing in seawater containing a compound beneficial microorganism preparation for a third time for hatching, to obtain brine shrimp nauplii carrying compound beneficial microorganisms;

[0009] The compound beneficial microorganism preparation at least includes lactic acid bacteria and butyric acid bacteria.

[0010] According to the above technical means, the complex beneficial microorganisms are first loaded in the brine shrimp nauplii, and then the complex beneficial microorganisms are successfully introduced into the macrobrachium rosenbergii larvae by using the brine shrimp nauplii as a carrier, so as to effectively improve the intestinal microflora of the macrobrachium rosenbergii larvae, and further improve the immune ability and feeding capacity of the macrobrachium rosenbergii larvae, promote the growth, and greatly improve the metamorphosis development success rate of the macrobrachium rosenbergii larvae; at the same time, the brine shrimp eggs are soaked in the first disinfectant to eliminate the pathogenic bacteria on the shell surface, and the brine shrimp nauplii are soaked in the second disinfectant for secondary disinfection to eliminate the pathogenic bacteria in the shell, so that the disinfection mode of the brine shrimp nauplii is optimized, the influence of the brine shrimp carrying pathogenic bacteria on the macrobrachium rosenbergii breeding is eliminated to the greatest extent, and the success rate of the macrobrachium rosenbergii larvae breeding is further improved.

[0011] Preferably, the mass ratio of the lactic acid bacteria and the butyric acid bacteria in the complex beneficial microorganism preparation is 7:3.

[0012] Preferably, the butyric acid bacteria is obtained by anaerobic fermentation of butyric acid bacteria freeze-dried powder, and the content of the butyric acid bacteria in the butyric acid bacteria fermentation liquor is 10 6 -10 7 cfu / mL; and the lactic acid bacteria is selected from lactic acid bacteria liquid, and the content of the lactic acid bacteria in the lactic acid bacteria liquid is 10 6 -10 7 cfu / mL.

[0013] Preferably, in the S1, the content of the hatched brine shrimp eggs in the ton of hatching salt water is between 1 kg and 1.5 kg.

[0014] Preferably, in the S1, the water temperature of the hatching salt water is between 30℃ and 32℃.

[0015] Preferably, in the S1, the pH value of the hatching salt water is between 8 and 9.

[0016] Preferably, in the S1, the dissolved oxygen content of the hatching salt water is greater than or equal to 5 mg / L.

[0017] Preferably, in the S1, the light intensity of the hatching process is between 1800 and 2200 Lx.

[0018] By reasonably controlling the light intensity, the change of water quality can be effectively managed, and the feeding amount of the larvae can also be ensured to be at an appropriate level, so as to realize the dual goals of water quality optimization and healthy growth of the larvae.

[0019] Preferably, in the S1, the first disinfectant is selected from a bleaching powder solution, and the concentration of the bleaching powder in the bleaching powder solution is between 800 and 1200 mg / L.

[0020] Preferably, in the S1, the first time is between 25 and 35 minutes.

[0021] Preferably, in the S1, the incubation time is between 24 and 30 hours.

[0022] Preferably, in the S2, the number of brine shrimp nauplii per milliliter of seawater is not less than 5,000.

[0023] Preferably, in the S2, the content of the compound beneficial microorganism preparation in the seawater is not less than 10 5 ind. / L.

[0024] Preferably, in the S2, the seawater is selected from sterilized seawater, and the salinity of the sterilized seawater is between 15‰ and 18‰.

[0025] By reasonably controlling the number of brine shrimp nauplii per milliliter of seawater and the content of the compound beneficial microorganism preparation in the seawater, it is effectively ensured that the brine shrimp nauplii can ingest the beneficial microorganisms into their bodies.

[0026] Preferably, in the S2, the second disinfectant is selected from trichloroisocyanuric acid powder solution, and the concentration of trichloroisocyanuric acid powder in the trichloroisocyanuric acid powder solution is between 1.3 and 1.7 mg / L.

[0027] Preferably, in the S2, the second time is between 20 and 30 minutes.

[0028] Preferably, in the S2, the third time is between 10 and 15 minutes.

[0029] Preferably, in the S1, it specifically comprises: the brine shrimp eggs are packed into 200-mesh silk screen bags and sealed, and then soaked in a bleaching powder solution with a concentration of 1000 mg / L for disinfection for 30 minutes, during which the brine shrimp eggs are turned over every 10 minutes, and then the brine shrimp eggs are repeatedly washed under flowing tap water to remove impurities and residual bleaching powder; the cleaned brine shrimp eggs are placed in a container with a conical bottom, the volume of the container is generally 0.5 cubic meters to 2 cubic meters, special salt for aquaculture is used to artificially prepare brine shrimp egg incubation seawater, the salinity of the brine shrimp egg incubation seawater is between 15‰ and 18‰, and the brine shrimp egg incubation seawater is poured into the container according to the proportion of 1 kilogram to 1.5 kilograms of brine shrimp eggs per ton of brine shrimp egg incubation seawater, so that the brine shrimp eggs are incubated, and the brine shrimp nauplii after incubation for 24 to 30 hours are collected according to the feeding time requirement, the air and light are stopped for 15 to 20 minutes before collection, the brine shrimp nauplii are made to sink to the bottom of the incubation tank, and then a 200-mesh screen bag is used to collect the brine shrimp nauplii by water or siphoning, thereby obtaining the brine shrimp nauplii.

[0030] The incubation container can be made of brick and concrete structure, glass steel material, pottery jar, or cement pipe, etc.

[0031] In the process of hatching, the water temperature of the hatching salt water is between 30-32℃, the pH of the hatching salt water is between 8-9, the dissolved oxygen content of the hatching salt water is greater than or equal to 5mg / L, and the light intensity in the hatching process is between 1800-2200Lx.

[0032] The purpose of stopping light and air before collection is to facilitate the separation of the brine shrimp egg shell and the hatched larvae.

[0033] Preferably, in S2, specifically comprising: the brine shrimp nauplii collected in S1 are again rinsed and soaked with 1.5mg / L trichloroisocyanuric acid powder solution for 25min, and the brine shrimp nauplii are turned over every 2min during the process, so as to eliminate the influence of pathogenic bacteria carried in the shell after hatching; and the brine shrimp nauplii are then repeatedly washed with sterile water for 2-3 times to remove residual trichloroisocyanuric acid powder; then the washed brine shrimp nauplii are put into a sterilized stainless steel barrel, 15‰-18‰ sterilized seawater is added, and the number of brine shrimp nauplii per milliliter of sterilized seawater is ensured to be not less than 5000, while aerating, a compound beneficial microorganism preparation is added into the water body in the stainless steel barrel, and the content of the compound beneficial microorganism preparation is ensured to be not less than 10 5 ind. / L, after the brine shrimp nauplii feed for 10-15min, the brine shrimp nauplii carrying beneficial microorganisms are collected by using a 200-mesh mesh bag, and the brine shrimp nauplii carrying beneficial microorganisms are obtained and put into a transfer barrel, sterile water is added and aerated for standby.

[0034] Preferably, the compound beneficial microorganism preparation comprises lactic acid bacteria and butyric acid bacteria, and the preparation method of the compound beneficial microorganism preparation is as follows: the purchased butyric acid bacteria freeze-dried powder is subjected to anaerobic fermentation to obtain a butyric acid bacteria fermentation liquor, and the bacterial content is determined; the purchased lactic acid bacteria liquid bacterial content is determined; and the butyric acid bacteria fermentation liquor and the lactic acid bacteria liquid are mixed according to the mass ratio of lactic acid bacteria: butyric acid bacteria = 7:3 to obtain the compound beneficial microorganism preparation.

[0035] The application also provides a breeding method of Macrobrachium rosenbergii, comprising the following steps:

[0036] The Macrobrachium rosenbergii larvae are arranged in the breeding water body, the brine shrimp nauplii carrying the compound beneficial microorganisms obtained by the hatching method are fed on the second day of the arrangement, and then the feeding amount is increased day by day until the Macrobrachium rosenbergii larvae develop to the eighth stage (Z8), and then the feeding amount of the egg paste is gradually increased, and the feeding amount of the brine shrimp nauplii carrying the compound beneficial microorganisms is gradually reduced, that is, the Macrobrachium rosenbergii larvae develop to the eighth stage (Z8), and then the feeding of the egg paste is mainly supplemented by the brine shrimp nauplii carrying the compound beneficial microorganisms.

[0037] According to the above technical means, the compound beneficial microorganisms are successfully sent into the bodies of the Macrobrachium rosenbergii juveniles by feeding the Artemia nauplii carrying the compound beneficial microorganisms to the Macrobrachium rosenbergii juveniles on the second day of the breeding and increasing the feeding amount day by day, so that the compound beneficial microorganisms are successfully sent into the bodies of the Macrobrachium rosenbergii juveniles by taking the Artemia nauplii as a carrier, the microbial flora in the bodies of the Macrobrachium rosenbergii juveniles is effectively improved, and then the health degree of the intestinal tract of the Macrobrachium rosenbergii juveniles in the breeding process is effectively improved, the immunity and the feeding capacity of the juveniles are improved, the disease resistance of the juveniles is enhanced, the metamorphic development time of the juveniles in the breeding process is shortened, and the success rate of the breeding is improved.

[0038] The specific feeding amount of the Artemia nauplii carrying the compound beneficial microorganisms which is increased day by day is determined according to multiple factors such as weather, water quality and juvenile feeding.

[0039] Preferably, the initial feeding density of the Artemia nauplii carrying the compound beneficial microorganisms in the breeding water body is 3×10 5 ~ 5×10 5 ind per 10 4 ind per 10

[0040] Preferably, the breeding method of the Macrobrachium rosenbergii specifically comprises the following steps:

[0041] The Macrobrachium rosenbergii juveniles are arranged in the breeding water body, the Artemia nauplii carrying the compound beneficial microorganisms is fed on the second day of the arrangement, the initial feeding density of the Artemia nauplii carrying the compound beneficial microorganisms in the breeding water body is 3×10 5 ~ 5×10 5 ind per 10 4 ind per 10

[0042] The beneficial effects of the present application are as follows:

[0043] 1) The incubation method of Daphnia, by first loading the complex beneficial microorganisms into the Daphnia nauplii, and then using the Daphnia nauplii as a carrier to successfully enter the complex beneficial microorganisms into the Macrobrachium rosenbergii larvae, thereby effectively improving the intestinal microbial flora of the Macrobrachium rosenbergii larvae, and further improving the immune function and feeding capacity of the Macrobrachium rosenbergii larvae, promoting the growth, and greatly improving the metamorphosis development success rate of the Macrobrachium rosenbergii larvae; at the same time, by using the first disinfectant to soak and disinfect the Daphnia eggs, the pathogenic bacteria on the surface of the shell are eliminated, and by using the second disinfectant to soak and disinfect the Daphnia nauplii, the pathogenic bacteria inside the shell are eliminated, and the disinfection method of the Daphnia nauplii is optimized, thereby eliminating the influence of the Daphnia carrying pathogenic bacteria on the Macrobrachium rosenbergii breeding to the greatest extent, and further improving the success rate of the Macrobrachium rosenbergii larvae breeding;

[0044] 2) The breeding method of Macrobrachium rosenbergii, by feeding the Daphnia nauplii loaded with complex beneficial microorganisms to the Macrobrachium rosenbergii larvae on the second day of breeding, and increasing the feeding amount day by day, thereby using the Daphnia nauplii as a carrier to successfully send the complex beneficial microorganisms into the Macrobrachium rosenbergii larvae, effectively improving the microbial flora in the Macrobrachium rosenbergii larvae, and further effectively improving the health of the intestinal tract of the Macrobrachium rosenbergii larvae during the breeding process, improving the immune function and feeding capacity of the larvae, enhancing the disease resistance of the larvae, promoting the growth of the larvae, shortening the metamorphosis development time of the larvae during the breeding process, and improving the success rate of breeding, which has popularization and application value in the field of aquaculture technology. DETAILED DESCRIPTION

[0045] The preferred embodiments of the present application will be described below with reference to the drawings. Based on the disclosure herein, those skilled in the art can easily understand other advantages and effects of the present application. The present application can also be implemented or applied in different specific embodiments, and various modifications or changes can be made to the details in the specification without departing from the spirit of the present application. It should be understood that the preferred embodiments are only for illustration of the present application, but not for limiting the protection scope of the present application.

[0046] The present application aims to disclose an incubation method of Daphnia and a breeding method of Macrobrachium rosenbergii, to solve the problem of low metamorphosis development rate of the larvae in the existing breeding method of Macrobrachium rosenbergii.

[0047] The incubation method of Daphnia comprises the following steps:

[0048] S1, soaking and disinfecting the Daphnia eggs in a first disinfectant for a first time, then washing with water and placing in a hatching brine with a salinity of 15‰-18‰ for hatching, to obtain Daphnia nauplii;

[0049] S2, soaking the brine shrimp nauplii in the second disinfectant for a second disinfection time, then washing with water and placing in seawater containing the compound beneficial microorganism preparation for a third time to obtain the brine shrimp nauplii carrying the compound beneficial microorganism;

[0050] The compound beneficial microorganism preparation at least comprises lactic acid bacteria and butyric acid bacteria.

[0051] By loading the compound beneficial microorganism into the brine shrimp nauplii first, and then using the brine shrimp nauplii as a carrier to make the compound beneficial microorganism successfully enter the Macrobrachium rosenbergii larvae, the intestinal microflora of the Macrobrachium rosenbergii larvae is effectively improved, and the body immunity and the feeding capacity of the Macrobrachium rosenbergii larvae are improved, the growth is promoted, and the metamorphosis development success rate of the Macrobrachium rosenbergii larvae is greatly improved; at the same time, by using the first disinfectant to soak the brine shrimp eggs, the pathogenic bacteria on the surface of the shell are eliminated, and by using the second disinfectant to soak the brine shrimp nauplii for the second time, the pathogenic bacteria inside the shell are eliminated, the disinfection mode of the brine shrimp nauplii is optimized, and the influence of the brine shrimp carrying pathogenic bacteria on the Macrobrachium rosenbergii breeding is eliminated to the greatest extent, and the success rate of the Macrobrachium rosenbergii larvae breeding is further improved.

[0052] In some embodiments, the mass ratio of the lactic acid bacteria and the butyric acid bacteria in the compound beneficial microorganism preparation is 7:3.

[0053] The butyric acid bacteria is obtained by anaerobic fermentation of butyric acid bacteria freeze-dried powder, and the content of the butyric acid bacteria in the butyric acid bacteria fermentation liquor is 10 6 -10 7 cfu / mL; and the lactic acid bacteria is selected from a lactic acid bacteria liquid, and the content of the lactic acid bacteria in the lactic acid bacteria liquid is 10 6 -10 7 cfu / mL.

[0054] In some embodiments, in S1, the content of the brine shrimp eggs in the incubation saltwater per ton is between 1 kg and 1.5 kg; the water temperature of the incubation saltwater is between 30°C and 32°C; and the pH value of the incubation saltwater is between 8 and 9.

[0055] In some embodiments, in S1, the light intensity of the incubation process is between 1800 and 2200 Lx.

[0056] In some embodiments, in S1, the first disinfectant is selected from a bleaching powder solution, the concentration of the bleaching powder in the bleaching powder solution is between 800 and 1200 mg / L; the first time is between 25 and 35 min; and the incubation time is between 24 h and 30 h.

[0057] In some embodiments, in S2, in order to ensure that as many as possible of the brine shrimp nauplii can ingest the beneficial microorganisms into their bodies, therefore, the number of the brine shrimp nauplii in each milliliter of seawater is not less than 5,000; the content of the compound beneficial microorganism preparation in the seawater is not less than 10 ind. / L; and the seawater is selected from sterilized seawater with a salinity of 15‰-18‰. 5

[0058] In some embodiments, in S2, the second disinfectant is selected from trichloroisocyanuric acid powder solution, the concentration of trichloroisocyanuric acid powder in the trichloroisocyanuric acid powder solution is between 1.3-1.7 mg / L, the second time is between 20-30 min, and the third time is between 10-15 min.

[0059] For example, in S1, it specifically includes: the brine shrimp eggs are packed into 200-mesh silk screen bags and sealed, soaked in a bleaching powder solution with a concentration of 1000 mg / L for 30 min, and turned over every 10 min during the soaking, then the brine shrimp eggs are repeatedly washed under flowing tap water to remove impurities and residual bleaching powder; the cleaned brine shrimp eggs are placed in a container with a conical bottom, the volume of the container is generally 0.5-2 cubic meters, a special salt for aquaculture is used to artificially prepare hatching salt water for the brine shrimp eggs, the salinity of the hatching salt water is between 15‰-18‰, and the hatching salt water is poured into the container according to the proportion of 1-1.5 kg of brine shrimp eggs per ton of hatching salt water, so that the brine shrimp eggs are hatched, and the brine shrimp nauplii hatched for 24-30 hours are collected according to the feeding requirements, the light is blocked and the air is stopped for 15-20 min before collection, the brine shrimp nauplii are collected by water or siphon with a 200-mesh screen bag, and the brine shrimp nauplii are obtained.

[0060] The hatching container can be made of brick-concrete structure, glass steel material, pottery jar or cement pipe, etc.

[0061] During the hatching process, the water temperature of the hatching salt water is between 30-32℃, the pH of the hatching salt water is between 8-9, the dissolved oxygen content of the hatching salt water is greater than or equal to 5 mg / L, and the light intensity during the hatching process is between 1800-2200 Lx.

[0062] ​In the incubation process of the brine shrimp nauplii, the brine shrimp eggs are soaked in 1000 mg / L bleaching powder solution for 30 minutes before hatching to effectively eliminate the pathogenic bacteria on the surface of the shell; at the same time, after the nauplii hatches, the brine shrimp nauplii are again soaked in 1.5 mg / L strong chlorine solution for 25 minutes to further effectively eliminate the pathogenic bacteria inside the shell, thereby maximizing the elimination of the carrying of pathogenic bacteria by the brine shrimp nauplii, and the practice has proved that both disinfections do not affect the activity of the brine shrimp nauplii, greatly inhibiting the carrying of bait pathogenic bacteria, which is of great significance to ensure the success rate of the breeding of Macrobrachium rosenbergii.

[0063] For example, in S2, specifically comprising: the brine shrimp nauplii collected in S1 are again soaked in 1.5 mg / L trichloroisocyanuric acid powder solution for 25 minutes, and are turned over every 2 minutes during the period to eliminate the influence of the pathogenic bacteria carried in the shell after the body hatches, and are then repeatedly washed with sterile water for 2-3 times to remove the residual trichloroisocyanuric acid powder; then the washed brine shrimp nauplii are put into a stainless steel barrel after disinfection, 15‰-18‰ sterilized seawater is added to ensure that the number of brine shrimp nauplii per milliliter of sterilized seawater is not less than 5000, aeration is carried out at the same time, and the compound beneficial microorganism preparation is added to the water body in the stainless steel barrel to ensure that the content of the compound beneficial microorganism preparation is not less than 10 5 ind. / L, after the brine shrimp nauplii feed for 10-15 minutes, the brine shrimp nauplii carrying beneficial microorganisms are collected with a 200-mesh mesh bag to obtain the brine shrimp nauplii carrying beneficial microorganisms, and are put into a barrel, sterile water is added and aeration is prepared.

[0064] For example, the compound beneficial microorganism preparation includes lactic acid bacteria and butyric acid bacteria, and the preparation method of the compound beneficial microorganism preparation is as follows: the purchased butyric acid bacteria freeze-dried powder is subjected to anaerobic fermentation to obtain butyric acid bacteria fermentation liquor, and the bacterial content is determined; the purchased lactic acid bacteria liquid bacterial content is determined. According to the mass ratio, the lactic acid bacteria: butyric acid bacteria = 7:3 ratio is mixed with the butyric acid bacteria fermentation liquor and the lactic acid bacteria liquid to obtain the compound beneficial microorganism preparation.

[0065] In some embodiments, a breeding method of Macrobrachium rosenbergii is also provided, comprising the following steps:

[0066] The Macrobrachium rosenbergii larvae are arranged in the breeding water body, and the brine shrimp nauplii carrying the compound beneficial microorganism obtained by the incubation method in any of the above embodiments are fed on the second day of the arrangement, and then the feeding amount is increased day by day until the Macrobrachium rosenbergii larvae develop to the eighth stage (Z8), and then the feeding amount of the egg paste is gradually increased and the feeding amount of the brine shrimp nauplii carrying the compound beneficial microorganism is gradually reduced, that is, the Macrobrachium rosenbergii larvae develop to the eighth stage (Z8), and then the feeding of the egg paste is mainly supplemented by the brine shrimp nauplii carrying the compound beneficial microorganism.

[0067] By feeding Artemia nauplii carrying a compound of beneficial microorganisms to the juvenile Macrobrachium rosenbergii starting on the second day of rearing, and gradually increasing the amount of feed each day, the compound of beneficial microorganisms was successfully delivered into the juvenile Macrobrachium rosenbergii using Artemia nauplii as a carrier. This effectively improved the microbial community in the juvenile Macrobrachium rosenbergii, thereby effectively improving the intestinal health of the juvenile Macrobrachium rosenbergii during the rearing process, enhancing the juveniles' immunity and feeding ability, strengthening their disease resistance, shortening the metamorphosis time of the juveniles during the rearing process, and increasing the success rate of rearing.

[0068] In some embodiments, the initial feeding density of Artemia nauplii loaded with a complex of beneficial microorganisms in the nursery water is 3 × 10⁻⁶. 5 ~5×10 5 only / 10 4 Between ind flea larvae.

[0069] An exemplary method for raising giant freshwater prawns specifically includes the following steps:

[0070] Giant freshwater prawn larvae were introduced into the nursery water. On the second day after larval emergence, Artemia nauplii larvae loaded with a complex of beneficial microorganisms, prepared in any of the above embodiments, were fed into the nursery water. The initial feeding density of Artemia nauplii larvae loaded with the complex of beneficial microorganisms in the nursery water was 3 × 10⁻⁶. 5 ~5×10 5 only / 10 4 Start feeding the zoea larvae, gradually increasing the amount each day until they finish eating within 1-1.5 hours. On the 8th day after the larvae develop to the fifth stage (Z5), a small amount of artificially prepared egg custard can be given, with the amount gradually increased. Once the zoea larvae reach the eighth stage (Z8), egg custard should be the primary food source, supplemented with Artemia nauplii.

[0071] For example, in the breeding method of giant freshwater prawns, the specific feed and feeding methods for each stage of larvae are as follows: Zoea larvae in stages I to VI are fed Artemia nauplii; zoea larvae in stages VII to IX are mainly fed Artemia nauplii, supplemented with egg custard; zoea larvae in stages X to XI are mainly fed egg custard, supplemented with Artemia nauplii; after stage XI, they are mainly fed egg custard and shrimp chips, supplemented with a small amount of Artemia nauplii. Artemia are fed daily from 08:00 to 09:00 and 14:00 to 15:00, with egg custard fed six times daily at 06:00, 10:00, 12:00, 16:00, 19:00, and 21:00. The feed and feeding methods for each stage of larvae are shown in Table 1.

[0072] Table 1. Feed and feeding methods for larvae at different stages.

[0073]

[0074]

[0075] In order to make the technical problems, technical solutions and beneficial effects solved by the present application clearer, the incubation method of Daphnia magna and the fry rearing method of Macrobrachium rosenbergii will be further described in detail below in combination with specific embodiments. Obviously, the specific embodiments described are only a part of the embodiments of the present application, rather than all the embodiments. The description of at least one exemplary embodiment below is actually only illustrative, but not as any limitation on the present application and its application. Based on the specific embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative labor fall within the scope of protection of the present application.

[0076] Unless otherwise specified in the specific embodiments, the technology or conditions are carried out according to the technology or conditions described in the literature in the art or according to the product instructions. Unless otherwise specified, the reagents or instruments used are all conventional products that can be obtained by purchase in the market.

[0077] Embodiment 1

[0078] An incubation method of Daphnia magna, comprising the following steps:

[0079] S1, the Daphnia magna eggs are packed into 200-mesh gauze bags and sealed, soaked in a bleaching powder solution with a concentration of 1000 mg / L for 30 min, and turned over every 10 min during the soaking, and then repeatedly washed under flowing tap water to remove impurities and residual bleaching powder;

[0080] The washed Daphnia magna eggs are placed in a container with a conical bottom, and the volume of the container is generally 0.5 cubic meters to 2 cubic meters;

[0081] Special salt for aquaculture is used to artificially prepare the hatching salt water for Daphnia magna eggs, and the salinity of the hatching salt water is about 16 ‰;

[0082] The hatching salt water is poured into the container at a ratio of 1 kg to 1.5 kg of Daphnia magna eggs per ton of hatching salt water, so that the Daphnia magna eggs are hatched. During the hatching process, the water temperature of the hatching salt water is between 30 ℃ and 32 ℃, the pH of the hatching salt water is between 8 and 9, the dissolved oxygen content of the hatching salt water is greater than or equal to 5 mg / L, and the light intensity during the hatching process is between 2000 Lx;

[0083] After 26 hours of hatching, the gas is stopped and the light is blocked for 15 min to 20 min, and then the Daphnia magna nauplii are collected by water or siphon with a 200-mesh mesh bag, so that the Daphnia magna nauplii are obtained;

[0084] S2. The nauplius larvae collected in S1 are rinsed and soaked in a 1.5 mg / L trichloroisocyanuric acid powder solution for 25 minutes for disinfection. During this period, they are turned over every 2 minutes to eliminate the influence of pathogens carried in the shell after hatching. Then, they are rinsed repeatedly with sterile water 2 to 3 times to remove residual trichloroisocyanuric acid powder.

[0085] The purchased lyophilized butyric acid bacteria powder was subjected to anaerobic fermentation to obtain butyric acid bacteria fermentation broth, and its bacterial content was determined; the bacterial content of the purchased lactic acid bacteria liquid was determined; the butyric acid bacteria fermentation broth and lactic acid bacteria liquid were mixed in a mass ratio of lactic acid bacteria:butyric acid bacteria = 7:3 to prepare a compound beneficial microbial preparation.

[0086] After rinsing, place the Artemia nauplii larvae into a sterilized stainless steel tank. Add sterilized seawater with a salinity of approximately 16‰, ensuring there are about 5500 Artemia nauplii larvae per milliliter of sterilized seawater. While aerating, add a compound beneficial microbial preparation to the water in the stainless steel tank. The concentration of the compound beneficial microbial preparation in the sterilized seawater should not exceed 10%. 6 Approximately 1 ind. / L. After the nauplius larvae have been feeding for 10-15 minutes, the larvae carrying beneficial microorganisms are collected using a 200-mesh mesh bag. The resulting larvae are then placed in a transfer tank, sterile water is added, and the tank is aerated for later use.

[0087] Example 2

[0088] A method for raising giant freshwater prawns specifically includes the following steps:

[0089] The areas marked as 4#, 5# and 6# are 13m² respectively. 2 Approximately 1.2 million giant freshwater prawn larvae were placed in the nursery water. On the second day after larval emergence, they were fed with Artemia nauplii larvae loaded with a complex of beneficial microorganisms, as prepared in Example 1. The feeding density of the Artemia nauplii larvae loaded with the complex of beneficial microorganisms in the nursery water was (4-5) × 10⁻⁶. 5 only / 10 4 Start by feeding Artemia nauplii, gradually increasing the amount each day until the nauplii are consumed within 1-1.5 hours. On the 6th-7th day after emergence, when the daphnia larvae reach stage six (Z6), a small amount of artificially prepared egg custard can be fed, with the amount gradually increased. Once the daphnia larvae reach stage eight (Z8), the diet should primarily consist of egg custard, supplemented with Artemia nauplii. Specific feeds and feeding methods for each larval stage are shown in Table 1.

[0090] Comparative Example 1

[0091] The traditional method for raising giant freshwater prawns includes the following steps:

[0092] About 1.2 million Macrobrachium rosenbergii juveniles were arranged in three water areas marked as 1#, 2# and 3# with an area of 13 m 2 5 4 ind Artemia nauplii, and then the feeding amount was increased day by day, and it was appropriate to finish feeding 1-1.5 hours after feeding; when the 6th day after the arrangement of juveniles, i.e. after the development of the daphnia-like juveniles to the fifth stage (Z6), a small amount of egg custard made artificially was fed, and the feeding amount was slowly increased. After the development of the daphnia-like juveniles to the eighth stage (Z8), egg custard was mainly fed, and Artemia nauplii was supplemented.

[0093] The whole operation and management of the breeding process in Example 2 and Comparative Example 2 were the same, and finally the final hatching amount and the end time of breeding of each pool were measured, and the results are shown in Table 1.

[0094] Table 1: Results of the breeding test

[0095]

[0096]

[0097] From Table 1, it can be seen that the average hatching amount of the three breeding pools (i.e. 1#, 2# and 3#) using the traditional breeding method of Macrobrachium rosenbergii in Comparative Example 1 was 645,000, the breeding rate was 52.1%, while the average hatching amount of the three test breeding pools (i.e. 4#, 5# and 6#) using the breeding method of Macrobrachium rosenbergii in Example 2 of the present application was 742,000, the breeding rate was 61.9%, and the breeding rate was increased by 9.2%. At the same time, the end time of breeding of the three breeding pools (i.e. 1#, 2# and 3#) using the traditional breeding method of Macrobrachium rosenbergii in Comparative Example 1 was 23.3 days on average, while the end time of breeding of the three test breeding pools (i.e. 4#, 5# and 6#) using the breeding method of Macrobrachium rosenbergii in Example 2 of the present application was 21 days on average, and the breeding cycle was shortened by 2 days, and there was no significant difference in the size of the individuals in the breeding, thereby proving that the breeding method of Macrobrachium rosenbergii of the present application, by feeding Artemia nauplii loaded with complex beneficial microorganisms to the Macrobrachium rosenbergii juveniles on the second day of the breeding, successfully sent the complex beneficial microorganisms into the body of the Macrobrachium rosenbergii juveniles, effectively improved the success rate of breeding, shortened the metamorphosis development time of the juveniles in the breeding process, and saved more than 10% of the total cost, thereby improving the economic benefit of Macrobrachium rosenbergii.

[0098] ​​In summary, the incubation method of Daphnia, by first loading the complex beneficial microorganisms in Daphnia nauplii, and then using Daphnia nauplii as a carrier to make the complex beneficial microorganisms successfully enter the Macrobrachium rosenbergii larvae, effectively improves the intestinal microflora of the Macrobrachium rosenbergii larvae, and further improves the body immunity and feeding capacity of the Macrobrachium rosenbergii larvae, promotes the growth, greatly improves the metamorphosis development success rate of the Macrobrachium rosenbergii larvae; at the same time, by using the first disinfectant to soak and disinfect the Daphnia eggs, the pathogenic bacteria on the surface of the shell are eliminated, and the second disinfectant is used to soak and disinfect the Daphnia nauplii twice, the pathogenic bacteria in the shell are eliminated, and the disinfection method of the Daphnia nauplii is optimized, so that the influence of the Daphnia carrying pathogenic bacteria on the Macrobrachium rosenbergii breeding is eliminated to the greatest extent, and the success rate of the Macrobrachium rosenbergii larvae breeding is further effectively improved.

[0099] The Macrobrachium rosenbergii breeding method of the application, by feeding the Daphnia nauplii loaded with complex beneficial microorganisms to the Macrobrachium rosenbergii larvae on the second day of breeding, and increasing the feeding amount day by day, the complex beneficial microorganisms are successfully sent into the body of the Macrobrachium rosenbergii larvae by using the Daphnia nauplii as a carrier, the microflora in the body of the Macrobrachium rosenbergii larvae is effectively improved, and the health degree of the intestinal tract of the Macrobrachium rosenbergii larvae during the breeding process is effectively improved, the immunity and feeding capacity of the larvae are improved, the disease resistance of the larvae is enhanced, the metamorphosis development time of the larvae during the breeding process is shortened, and the success rate of the breeding is improved, which has popularization and application value in the field of aquaculture technology.

[0100] The above examples are only preferred embodiments for fully illustrating the present application, and the protection scope of the present application is not limited thereto. Any equivalent replacement or transformation of the present application based on the present application is within the protection scope of the present application.

Claims

1. A method for breeding Macrobrachium rosenbergii, characterized in that, The method comprises the following steps: arranging Macrobrachium rosenbergii larvae in a breeding water body, feeding the compound beneficial microorganism-loaded Artemia nauplii on the second day of the arrangement, then increasing the feeding amount day by day until the Macrobrachium rosenbergii larvae develop to the eighth stage (Z8), and then gradually increasing the feeding amount of egg yolk and gradually reducing the feeding amount of the compound beneficial microorganism-loaded Artemia nauplii; The incubation method of the compound beneficial microorganism-loaded Artemia nauplii comprises the following steps: S1, soaking the Artemia eggs in a first disinfectant for a first disinfection time, then washing with water, and then placing in a hatching brine with a salinity of 15‰-18‰ to hatch, to obtain Artemia nauplii; S2, soaking the Artemia nauplii in a second disinfectant for a second disinfection time, then washing with water, and then placing in seawater containing a compound beneficial microorganism preparation for a third time to incubate, to obtain compound beneficial microorganism-loaded Artemia nauplii, and placing in a transfer barrel, adding sterile water and aeration for standby; The compound beneficial microorganism preparation at least comprises lactic acid bacteria and butyric acid bacteria; The mass ratio of the lactic acid bacteria and the butyric acid bacteria in the compound beneficial microorganism preparation is 7:3-6:4; In S1, the first disinfectant is selected from a bleaching powder solution, the concentration of the bleaching powder in the bleaching powder solution is between 800-1200 mg / L, the first time is between 25-35 min, and the hatching time is between 24-30 h; In S2, the second disinfectant is selected from a trichloroisocyanuric acid powder solution, the concentration of the trichloroisocyanuric acid powder in the trichloroisocyanuric acid powder solution is between 1.3-1.7 mg / L, the second time is between 20-30 min, and the third time is between 10-15 min.

2. The method for breeding Macrobrachium rosenbergii according to claim 1, characterized in that, In S1, The content of the Artemia eggs in the hatching brine per ton is between 1-1.5 kg; And / or, the water temperature of the hatching brine is between 30-32℃; And / or, the pH value of the hatching brine is adjusted to be between 8-9 by using NaHCO3; And / or, the dissolved oxygen content of the hatching brine is greater than or equal to 5 mg / L; And / or, the light intensity during the hatching process is between 1800-2200 Lx.

3. The method for breeding Macrobrachium rosenbergii according to claim 1, characterized in that, In S2, The number of the Artemia nauplii per milliliter of seawater is not less than 5,000; And / or, the content of the compound beneficial microorganism preparation in seawater is not less than 10 5 ind. / L; And / or, the seawater is selected from sterilized seawater, and the salinity of the sterilized seawater is between 15‰-18‰.

4. The method for the culture of Macrobrachium rosenbergii according to claim 1, characterized in that, The initial feeding density of the brine shrimp nauplii carrying the complex beneficial microorganisms in the water body for breeding is 3 x 104 5 ~5 x 104 5 ind / 10 4 ind per liter of the worm-like larvae.

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