The application belongs to the technical field of
microorganism detection, and particularly relates to a primer set for detecting
drug-
resistant bacteria and application thereof, wherein the primer set comprises one upstream primer, one downstream primer and one probe; the sequence of the upstream primer is shown as SEQ ID NO. 1; the sequence of the downstream primer is shown as SEQ ID NO. 2; and the sequence of the probe is shown as SEQ ID NO. 6. Based on the primer set, the application establishes a method for detecting azolane
ketone drug-resistant
gene optrA The fluorescent RAA detection method can complete amplification within 21 min under isothermal conditions at 37 DEG C, the primer set has high specificity, and the RAA amplification result can be observed by
ultraviolet analysis instrument with naked eyes, so that the possibility of
rapid detection in basic areas is provided.