Polypeptide capable of inhibiting and killing multiple medicine-resisting bacteria

A technology for drug resistance and bacteria, applied in the field of new polypeptides that can inhibit and kill a variety of drug-resistant bacteria, and new polypeptides that can kill and inhibit bacteria

Active Publication Date: 2014-10-01
冯小荣 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The terrible thing about this germ is not its lethality to people, but its resistance t

Method used

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  • Polypeptide capable of inhibiting and killing multiple medicine-resisting bacteria
  • Polypeptide capable of inhibiting and killing multiple medicine-resisting bacteria
  • Polypeptide capable of inhibiting and killing multiple medicine-resisting bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] The minimum inhibitory concentration (MIC) was evaluated by the micro broth dilution method.

[0072] A. Preparation of antibacterial drugs and culture medium

[0073] The preparation of antibacterial drugs and medium is the same as the macro broth dilution method.

[0074] Antimicrobial drug concentration range for drug susceptibility test: According to the NCCLS antimicrobial drug susceptibility test operating standard, the drug concentration range should include the value of drug resistance, intermediary and sensitivity cut-off points.

[0075] Medium: NCCLS recommends using Mueller-Hinton (MH) broth, pH 7.2~7.4. Aerobic bacteria and facultative anaerobes grow well in this medium. To prepare the inoculum, use an inoculating loop to pick 3 to 5 colonies of similar morphology to be tested, inoculate them in 4 to 5 ml of hydrolyzed casein (MH) broth, and incubate at 35°C for 2 to 6 hours. After enrichment, the logarithmic growth phase bacterial liquid is corrected with normal...

Embodiment 2

[0086] Minimum bactericidal concentration test (MBC)

[0087] Minimum bactericidal concentration (MBC): The minimum drug concentration required to kill 99.9% (3 orders of magnitude) of the test microorganisms.

[0088] Double dilution to measure the minimum bactericidal concentration: it is to halve the drug concentration in turn (multiple dilution), for example, do MBC in a certain row / column of a 96-well plate, add 10μl of drug solution to each well, and the first well of drug solution concentration It is 100μg / mL, the second well is 50μg / mL, the third is 25μg / mL... The operation is like adding 20μl liquid medicine to the first hole, sucking 10μl liquid medicine from the first hole to the second hole, adding 10μl culture solution, after mixing, sucking 10μl from it and adding it to the third hole, then adding culture solution 10μl, mix well... At the lowest concentration, there are no live bacteria, this concentration is MBC.

[0089] See the result Figure 1~5 .

[0090] Among ...

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Abstract

The invention relates to polypeptide capable of inhibiting and killing bacteria, and in particular relates to multiple medicine-resisting bacteria. The polypeptide is remarkable in effect of killing or inhibiting methicillin-resistant staphylococcus epidermidis, klebsiella pneumoniae, baumanii and/or escherichia coli. The invention further relates to a composition with the defined polypeptide, and an application of the defined polypeptide and the composition thereof in preparation of medicines and in killing or inhibiting of multiple in-vitro medicine-resisting bacteria.

Description

Technical field [0001] The present invention relates to novel polypeptides, especially novel polypeptides for killing and inhibiting bacteria, in particular a group of novel polypeptides capable of inhibiting and killing multiple drug-resistant bacteria. Background technique [0002] Super bacteria refer to bacteria that have become more and more resistant due to the abuse of antibiotics. Their resistance is so strong that various antibiotics have no effect on them, so this type of bacteria is collectively called super bacteria. Super bacteria also specifically refer to trimethicillin-resistant (methicillin) Staphylococcus aureus (MRSA), which is a type of skin bacteria. It is difficult to diagnose and treat because it is difficult to kill with general antibiotics. Super bacteria is also a kind of drug-resistant bacteria. This super bacteria can cause thick sores and vesicles on people, and even gradually cause muscle necrosis. What's more frightening is that antibiotics do not ...

Claims

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Application Information

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IPC IPC(8): C07K7/06C07K7/08C07K14/00C07K1/06C07K1/04A61K38/08A61K38/10A61K38/16A61P31/04A01N47/44A01P1/00
CPCY02A50/30
Inventor 冯小荣彭千芮冯卫星
Owner 冯小荣
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