Glucose oxidase and hemoglobin loaded nanoreactor as well as preparation method and application thereof

A glucose oxidase and nanoreactor technology, which is applied in the field of nanomaterial preparation and methods, can solve problems such as applications that have not yet been reported, and achieve the effects of significant bacteriostatic effect, simple preparation method, and enhanced glucose oxidase activity.

Pending Publication Date: 2021-08-17
QINGDAO UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the application of glucose oxidase and hemoglobin-loaded nanoreactors based on the zeolite im

Method used

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  • Glucose oxidase and hemoglobin loaded nanoreactor as well as preparation method and application thereof
  • Glucose oxidase and hemoglobin loaded nanoreactor as well as preparation method and application thereof
  • Glucose oxidase and hemoglobin loaded nanoreactor as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] This embodiment relates to the preparation method of a nanoenactor loaded with glucose oxidase and hemoglobin, and the specific steps are as follows:

[0025] S1, weighing 0.11 g of apodium acetate is dissolved in 37 ml ultrapure water, thoroughly stirring dissolved; 1.23 g 2-methylimidazole is sungined in 37 ml ultra-pure water, and stirring is thoroughly stirred;

[0026] S2, mixing the solution obtained by step S1 is uniform, adding 600 microliters of concentration of 50 mg / ml of hemoglobin aqueous solution and 600 microlitudes of 50 mg / ml of glucose oxide aqueous solution, ultrasonic treatment under a nitrogen gas. 10 minutes. The ultrasonic power is 400 W, then incubated under room temperature for 12 hours;

[0027] S3, the solution obtained by step S2 is centrifuged at 5000 rpm for 10 minutes, and the precipitate is collected, and the precipitate is washed 3 times with ultrapure water;

[0028] S4, the precipitate obtained by step S3 is freeze and dried for 10 hou...

Embodiment 2

[0032] This embodiment relates to the preparation method of a nanoenactor loaded with glucose oxidase and hemoglobin, and the specific steps are as follows:

[0033] S1, weigh 0.01 g of apodium acetate is dissolved in 30 ml ultrapure water, thoroughly stirred and dissolve; 1.1 grams of 2-methylimidazole dissolved in 30 ml of ultra-pure water, thoroughly stirred and dissolved;

[0034] S2, mixing the two solutions obtained in step S1, stir well, add 60 microliters of concentration of 50 ml / ml of hemoglobin aqueous solution and 60 microlitudes of 50 mg / ml of glucose oxide aqueous solution, under conditions of nitrogen gas Ultrasound treatment for 5 minutes, ultrasonic power is 400 W, and then incubated for 6 hours under room temperature;

[0035] S3, the solution obtained by step S2 is centrifuged at 2000 rpm for 5 minutes, and the precipitate is collected, and the precipitate is washed 3 times with ultrapure water;

[0036] S4, the precipitate obtained by step S3 is freeze dry ...

Embodiment 3

[0038] This embodiment relates to the preparation method of a nanoenactor loaded with glucose oxidase and hemoglobin, and the specific steps are as follows:

[0039] S1, 1.2 g of zinc acetate is dissolved in 200 ml of ultra-pure water, thoroughly stirred and dissolve; 2.4 g of 2-methylimidazole is integrated into 200 ml supermoth water, thoroughly stirred and dissolve;

[0040] S2, mixing the solution obtained by step S1 is uniform, adding 120 microliters of concentration of 50 ml / ml of hemoglobin aqueous solution and 120 microlitudes of 50 mg / ml of glucose oxide aqueous solution, ultrasonic treatment under a nitrogen gas for 15 minutes. The ultrasonic power is 400 W, then incubated under room temperature for 12 hours;

[0041] S3, the solution obtained by step S2 is centrifuged at 8000 rpm for 15 minutes, and the precipitate is collected, and the precipitate is washed 3 times with ultrapure water;

[0042] S4, the precipitate obtained by step S3 was freeze and dried for 12 ho...

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Abstract

The invention belongs to the technical field of nano-material preparation and bacterial infection resistance, and relates to a glucose oxidase and hemoglobin loaded nano-reactor and a preparation method and an application thereof, the structure of the nano-reactor is a zeolite imidazate skeleton structure simultaneously loading glucose oxidase and hemoglobin, and the nano-reactor can be applied to bacterial infection resistance, and particularly, bacterial infection of wounds of diabetics can be controlled, and wound healing is promoted; the nano-reactor has good biocompatibility and environmental friendliness, is more remarkable in antibacterial effect, can achieve a good antibacterial effect without adding exogenous hydrogen peroxide, has a remarkable bactericidal effect on methicillin-resistant staphylococcus aureus and escherichia coli producing extended-spectrum beta-lactamase, and can efficiently promote the wound healing caused by multi-drug-resistant bacterial infection of the diabetics, and the market prospect is extremely wide.

Description

Technical field: [0001] The present invention belongs to the nanomaterial preparation and antibacterial infection technology, which relates to a nano-reactor having a negative glucose oxidase and hemoglobin and a preparation method and application thereof. Based on the metal organic frame structure through the package method load-bearing catalytic system, high The catalytic active nano reactor has a significant increase in activity than natural enzyme activity, which can control multiple drug-resistant bacterial infections, and promote the healing of diabetic wounds. Background technique: [0002] At present, bacterial infection is a common complications in patients, which seriously affects the recovery of surgery patients, and has caused a heavy economic burden. Diabetes patients, due to its high blood sugar levels, can provide sufficient nutrients for bacteria, so the healing of diabetes patients has more difficulties. Escherichia coli and golden Staphylococcus aureus are commo...

Claims

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Application Information

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IPC IPC(8): A61K38/44A61K38/42A61K47/22A61P31/04A61P17/02B82Y5/00B82Y40/00
CPCA61K38/443A61K38/42A61K47/22A61P31/04A61P17/02B82Y5/00B82Y40/00C12Y101/03004Y02A50/30
Inventor 许元红牛海涛李丹霞陈涛牛玉生
Owner QINGDAO UNIV
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