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Application of polypeptide RK12 in preparation of medicine for treating acne

An RK12, acne technology, applied in the field of biopharmaceuticals, can solve the problems of increased severity, scar formation, and difficult to treat inflammatory acne, and achieve the effects of promoting angiogenesis, changing permeability, and low drug resistance

Active Publication Date: 2022-03-22
成都佩德生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Inflammatory acne is difficult to treat, usually increases in severity and can lead to scarring

Method used

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  • Application of polypeptide RK12 in preparation of medicine for treating acne
  • Application of polypeptide RK12 in preparation of medicine for treating acne
  • Application of polypeptide RK12 in preparation of medicine for treating acne

Examples

Experimental program
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Effect test

Embodiment 1

[0058] Anti-Propionibacterium acnes Study of Small Molecular Polypeptide RK12

[0059] 1. Preparation of small molecule polypeptide RK12

[0060] What the present invention adopts is that α-amino uses Fmoc (9-fluorenylmethoxycarbonyl) solid-phase chemical synthesis method to synthesize the antimicrobial peptide whose amino acid sequence is RWWRFFKFWWKK, first the resin is expanded (the purpose of the expanded resin is to expose the active groups on the resin come out), and then add 2.5 mL of 20% piperidine to the expanded resin for deprotection, and at the same time activate the amino acid. Add the activated amino acid to the resin that has been deprotected and cleaned for coupling. When the coupling is completed, drain the liquid in the tube and wash it with DMF 8 times. After all the synthesis is completed, transfer the resin to the cracking tube and add the cracking solution (TFA:phenol:Tis:EDT=87.5:5:5:2.5) for lysis. Finally, add glacial ether to the lysate to 45ml, cen...

Embodiment 2

[0077] Research on anti-inflammation of small molecule polypeptide RK12

[0078] (1) In order to study the anti-inflammatory effect of RK12 on P. acnes-induced skin diseases, the present invention verified the anti-inflammatory effect of the peptide by monitoring the changes in the ears of animals using an inflammatory animal model treated with P. acnes.

[0079] Six-week-old female Kunming mice (22-25 g) (n=5) were randomly divided into 5 groups, with 5 mice in each group. like Figure 3a Shown is the group injected with PBS in the mouse ear endothelium, as Figure 3b Shown is the blank control, and the injection concentration in the ear endothelium of the remaining three groups is 1×10 7 CFU / 20 μL of P. acnes. After that, RK12 (20, 100, 200 μg) will be administered respectively (such as Figure 3c , 3d , 3e) and clindamycin (0.05μg) (as Figure 3f shown) on the surface of the right ear. After 72 hours, the animals were sacrificed by cervical dislocation and the ears w...

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Abstract

The invention provides application of polypeptide RK12 in preparation of a medicine for treating acne. The new application of the polypeptide RK12 is used for preparing the medicine for treating the acne, the polypeptide RK12 has a remarkable inhibition effect on propionibacterium acnes and clindamycin-resistant propionibacterium acnes, and compared with antibiotics, the polypeptide RK12 is low in drug resistance, has concentration and time dependence, and has a good application prospect in preparation of medicines for treating the acne and the clindamycin-resistant propionibacterium acnes. The research result of an in-vitro antibacterial spectrum experiment also proves that the composition can rapidly inhibit the growth of propionibacterium acnes and clindamycin-resistant propionibacterium acnes, the research result of an in-vitro anti-inflammatory experiment also proves that the composition has an obvious anti-inflammatory effect, and the composition is not easy to generate drug-resistant bacteria due to an antibacterial mechanism different from that of antibiotics. According to the new strategy and thought for treating acne provided by the invention, the antibacterial action mechanism is helpful for clinically limiting the influence of antibiotic resistance.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to the application of a polypeptide RK12 in the preparation of acne-treating medicines. Background technique [0002] Acne vulgaris is a chronic bacterial sebaceous gland inflammatory disease that typically develops on the face and upper trunk (front chest and back) of adolescents and is characterized by the formation of comedones, papules, nodules, pustules, and cysts. It is a common skin disease affecting 35% to more than 90% of those infected during adolescence. Acne lesions are generally classified as noninflammatory (open and closed comedones) or inflammatory (papules and pustules). Inflammatory acne is difficult to treat, usually increases in severity and can lead to scarring. The current study suggests that the pathophysiology of acne involves three factors: seborrheic hypersebum, abnormal keratinization of the pilaris, and proliferation of Propionibacterium acne...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/10A61P17/10A61P17/02A61P29/00
CPCA61K38/10A61P17/10A61P17/02A61P29/00Y02A50/30
Inventor 谷陟欣黄彪
Owner 成都佩德生物医药有限公司
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