102 results about "Propionibacterium sp." patented technology

The invention discloses propionibacterium capable of obtaining high-yield propionic acid through compound mutation screening and a method for fermenting propionic acid by using the propionibacterium. The high-yield propionic acid strain YCBS010 (CGMCC No.31160) is obtained by carrying out compound mutagenesis on acid-producing propionibacterium CGMCC 1.2232 serving as an original strain through microwave-ultrasonic combined mutagenesis and compound normal-pressure and room-temperature plasma (ARTP) mutagenesis means. According to the invention, YPA15 is used as a starting strain, microwave and ultrasonic combined mutagenesis is carried out for 100 seconds to screen a bacterial strain with increased propionic acid yield, then ARTP mutagenesis is carried out for 90 seconds to screen out a high-yield mutant strain YCBS010, fermentation in a 1-ton tank verifies that the highest propionic acid yield reaches 65.87 g / L and the fermentation intensity is 0.73 g / L.h after fermentation is carried out for 90 hours, and the bacterial strain has better industrial fermentation potential.

The invention belongs to the technical field of biological fermentation, and relates to a high-bacteriostatic-activity propionibacterium scherichia subspecies and application thereof, in particular to a propionibacterium scherichia subspecies which is classified and named as Propionibacterium fredenreichiii subsp. Scherichia CJPF0001, and the preservation number of the propionibacterium scherichia subspecies is CCTCC (China Center for Type Culture Collection) No.M 20242477. The invention further relates to a preparation method of the propionibacterium scherichia subspecies and application of the propionibacterium scherichia subspecies. The fermentation product of the strain has high protein and high bacteriostatic activity, can inhibit the growth of common spoilage microorganisms, and has the effects of improving the nutritional value of food and prolonging the shelf life of food and the like.

The invention relates to Penicillium decumbens C01 and application of the Penicillium decumbens C01, and belongs to the technical field of microorganisms. The penicillium decumbens C01 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the penicillium decumbens C01 is GDMCC NO.66456. The strain is obtained by separating, purifying and screening soil samples collected from botanical gardens in South China of Guangzhou; tests show that the bacteriostatic active substance generated by fermentation of the strain has a good bacteriostatic effect on staphylococcus epidermidis, staphylococcus aureus, escherichia coli, propionibacterium acnes, candida albicans and pseudomonas aeruginosa, and the bacteriostatic active substance has the characteristic of high temperature resistance and excellent preservative effect; the antibacterial preservative can be used as an antibacterial preservative raw material, is applied to the fields of food, health care products or daily chemical products and the like, and has wide application prospects and important conversion research values.

The invention belongs to the technical field of biology, and particularly relates to an antibacterial peptide for inhibiting propionibacterium acnes and application of the antibacterial peptide. The antibacterial peptide for inhibiting propionibacterium acnes is derived from marine organism hylophila, and the amino acid sequence of the antibacterial peptide is shown as SEQ ID NO.1. The amino acid sequence is novel, has remarkable antibacterial activity on propionibacterium acnes, has the advantages of low hemolysis toxicity, low cytotoxicity and high safety, and can be used for preparing the antibacterial peptide for inhibiting propionibacterium acnes. Due to the characteristics, the compound has huge application potential in the aspect of treating acnes caused by propionibacterium acnes or removing acnes.

The present invention discloses a natural plant extract composition for preventing hair loss and fixing hair. Through the reasonable proportion and synergistic effect of seven kinds of plant extracts, including Hypericum perforatum extract, Guaiacaceae extract, Mangostana mangosteen peel extract, mango leaf extract, Polygonum multiflorum extract, Platycladus orientalis leaf extract, and ginger extract, 5α-reductase and main microorganisms such as Malassezia furfur, Propionibacterium acnes and Staphylococcus aureus that cause seborrheic alopecia are effectively inhibited, thereby reducing the probability of seborrheic alopecia. The beneficial ingredients in the seven kinds of plant extracts have the effects of promoting blood circulation and regulating scalp oil secretion, can promote hair growth, and improve the scalp environment. In addition, mango leaf extract and Polygonum multiflorum extract are rich in vitamins and nutrients, can nourish hair, enhance the elasticity and gloss of hair, and reduce hair breakage and shedding. The present invention also discloses the preparation method and application of the above-mentioned natural plant extract composition for preventing hair loss and fixing hair.

The invention relates to the technical field of biology, in particular to a method for producing vitamin B12 through fermentation of propionibacterium shermangii, which comprises the following steps: inoculating propionibacterium shermangii into a seed culture medium to culture a seed solution; inoculating the seed solution into a fermentation culture medium for fermentation; after fermentation is finished, thalli are removed in a centrifugal separation or filtration mode, a fermentation solution is subjected to ultrafiltration concentration, a concentrated solution is purified through ion exchange chromatography or affinity chromatography, and a vitamin B12 crude product is obtained; and further purifying by adopting a crystallization method to obtain a high-purity vitamin B12 finished product. According to the method for producing the vitamin B12 through fermentation of the propionibacterium shermannii, the yield and the purity of the vitamin B12 are remarkably improved and the production cost is reduced by accurately controlling fermentation conditions, optimizing culture medium components, introducing an intelligent regulation and control system and adopting a specific purification process, so that the method is suitable for industrial production. The problems of low yield, unstable purity, difficult production process control and the like of the vitamin B12 in the prior art are solved.

The invention relates to the technical field of skin care products, in particular to an acne removal composition capable of acting on a biological membrane as well as a preparation method and application of the acne removal composition. The caesalpinia spinosa gum and the compound fermentation liquor are compounded according to a certain proportion, so that the synergistic interaction effect can be achieved, on one hand, the effect of inhibiting the formation of a propionibacterium acnes biological membrane can be better, and on the other hand, the generated propionibacterium acnes biological membrane can be disintegrated; on the other hand, the effects of removing acnes, relieving and controlling oil can be achieved; preferably, lactobacillus plantarum, lactobacillus acidophilus and lactobacillus reuteri are specifically selected for compound fermentation, so that the activity of the obtained composition is better, and the effect of inhibiting repeated acnes is more remarkable; according to the invention, reasonable active ingredient matching and a special preparation process are adopted, the effect of comprehensively inhibiting acne recurrence can be achieved through combined action of disintegration of the biological membrane dimension of generated propionibacterium acnes, relieving and oil control and adjustment of skin micro-ecological balance dimension, and the effect is more remarkable.

There is provided a composition that comprises a propionic acid bacterium or a composition that comprises an oligosaccharide. The composition that comprises a propionic acid bacterium or the composition that comprises an oligosaccharide can suppress an increase of body weight of a subject by having the subject ingest the composition.

The invention provides a lactobacillus plantarum 18 which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC No.31277. The lactobacillus plantarum 18 has the advantages that the lactobacillus plantarum 18 is prepared from lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum and lactobacillus plantarum; the plant lactobacillus 18 can inhibit growth of propionibacterium acnes, inhibit expression of virulence factors (biological membranes, lipase and the like) of the propionibacterium acnes, relieve inflammation, inhibit autophagy of cells, scavenge free radicals and inhibit activity of tyrosinase, so that the plant lactobacillus 18 has the effects of resisting acnes, dermatitis and oxidation and whitening skin. The plant lactobacillus 18 or the metabolite of the plant lactobacillus 18 can be used for preparing products with the effects or effects.

The present application relates to a strain of Penicillium decumbens (P. Penicillium decumbens ) C01 and its application, and belongs to the field of microbial technology. The Penicillium decumbens C01 disclosed by the present application has been preserved in the Guangdong Microbial Digital Culture Collection Center, and its preservation number is GDMCC NO.66456. The strain is isolated, purified and screened from a soil sample collected in the garden area of Guangzhou South China Botanical Garden. Tests show that the bacteriostatic active substance produced by fermentation of the strain has good bacteriostatic effect on Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Propionibacterium acnes, Candida albicans and Pseudomonas aeruginosa. Moreover, the bacteriostatic active substance has high-temperature resistance and excellent preservative effect, and can be used as a bacteriostatic and preservative raw material, applied in the fields of food, health care products or daily chemical products, and has broad application prospect and important transformation research value.

There is provided a composition that comprises a propionic acid bacterium or a composition that comprises an oligosaccharide. The composition that comprises a propionic acid bacterium or the composition that comprises an oligosaccharide can suppress an increase of body weight of a subject by having the subject ingest the composition.

The invention discloses an oil-controlling, soothing and repairing composition and a preparation method thereof. The oil-controlling, soothing and repairing composition is prepared from a herba portulacae extract, a herba centellae extract, a baropetalum chinense leaf extract and a plankton extract. According to multiple experimental researches, the composition obtained by compounding the extracts has excellent effects of controlling oil, inhibiting activity of propionibacterium acnes, relieving and repairing, is mild and non-irritant, and has a good popularization and application prospect.

The present application relates to the field of microbial technology, and particularly relates to a gene editing method of propionibacterium acnes VB208 and application thereof. Propionibacterium acnes ) on February 20, 2023, and is preserved in the China General Microbiological Culture Collection Center, with a preservation number of CGMCC No.26576. The propionibacterium acnes screened by the present application has the characteristics of skin surface occupation, high conversion efficiency, gene modification potential and efficient expression of exogenous proteins, and can be used as an engineering strain. Furthermore, a genetic operation system suitable for the strain can be established by using the propionibacterium acnes, and the application prospect is wide.

The present invention provides a compound essential oil, an anti-corrosion system containing the same, and their uses. The compound essential oil is composed of the following components: wild chrysanthemum essential oil, jasmine essential oil, patchouli essential oil, and lemon-aldehyde-type camphor leaf essential oil. The compound essential oil has the effect of significantly inhibiting the growth of Propionibacterium acnes, Staphylococcus aureus, and Malassezia for a long time. The present invention also provides a compound anti-corrosion system, which contains the above compound essential oil, 1,2-hexanediol, and caprylyl glycol.

The invention relates to hair-blacking essence and a preparation method thereof. Screening a symbiotic bacterium metabolism extract with small molecule peptide metabolism regulation capability; by combining low-concentration azelaic acid and zinc lactate components, scalp grease oxidation and abnormal proliferation of propionibacterium are inhibited; the expression of IL-10 anti-inflammatory factors is induced, the expression of TNF-alpha and IL-6 is reduced, and the hair follicle microinflammation state is reduced; a Wnt / beta-catenin pathway activator is used for inducing the melanin stem cells to be in a static state; the FGF-2 and the EGF are combined in a micro-dose manner to promote the amplification into precursor cells; mITF regulation peptides and a small amount of trace copper ions are supplemented to induce directional differentiation into mature melanocytes; a microcapsule carrier system with a three-layer structure; the penetration efficiency is improved; antioxidative peptide rich in reduced glutathione, NAD and a promoting factor are used, so that melanocytes are protected from oxidative damage; a coenzyme Q10 type mitochondrial homeostasis regulator is introduced, so that the energy metabolism is improved, the cell aging is delayed, the targeting property is strong, and the effect is accurate.

This invention discloses a strain of *Propionibacterium freundii* and its application in propionic acid production. The strain was deposited at the China Center for Type Culture Collection on October 22, 2025, with accession number CCTCC M 20252289. This invention obtains a high-propionic acid-producing *Propionibacterium freundii* strain through screening from nature. Its 16S RNA was identified as *Propionibacterium freundii*. This strain is a safe edible strain approved by the Ministry of Health. Through optimization of its culture and fermentation conditions, the final propionic acid yield can reach over 50 g / L, the glucose conversion rate can reach 60%, and there is no lactic acid accumulation, meeting the requirements of industrial production. Furthermore, through fermentation optimization, the content of other acids can be effectively controlled, resulting in propionate products of different specifications that meet market demands.

The invention relates to a propionibacterium acnes selective culture medium and a preparation method thereof, the culture medium comprises casein pancreatin digest, meat and stomach enzyme digest, heart and pancreatin digest, yeast extract powder, corn starch, sodium chloride, agar and defiberized sheep blood, and the volume is fixed with distilled water. Compared with an existing culture medium, the culture medium has the advantages of being reliable in detection and high in propionibacterium acnes separation rate and has a good application prospect.

A peptide exhibiting both antibacterial and anti-inflammatory activities or a derivative thereof, including one of the following sequences: an amino acid sequence of SEQ ID NO: 1 or a variant thereof, and an amino acid sequence of SEQ ID NO: 2 or a variant thereof. The peptides synthesized in this disclosure exhibit antibacterial and anti-inflammatory effects, inhibiting one or more of the following: Escherichia coli, Staphylococcus aureus, Propionibacterium acnes, Candida albicans, as well as the expression of IL-6, IL-1B, CCL-2, IL-1, and NO.

The application relates to the technical field of biological medicine, in particular to a compound with a conjugated structure containing an alpha, beta-unsaturated aldehyde and application thereof. The compound can covalently modify Sortase A enzyme of propionibacterium acnes through alpha, beta-unsaturated aldehyde, inhibit transpeptidation catalysis and adhesion of gram-positive bacteria (for example, methicillin-resistant staphylococcus aureus, streptococcus mutans and propionibacterium acnes), block anchoring of membrane proteins, inhibit growth of gram-positive bacteria or kill bacteria; conjugated groups with different electric effects and steric effects are screened to adjust the covalent modification efficiency of alpha, beta-unsaturated aldehyde and Sortase A enzyme, strengthen the activity of the compound in inhibiting Sortase A enzyme, thereby improve the antibacterial activity of the compound, and further make the compound with the conjugated structure containing the alpha, beta-unsaturated aldehyde in the application be applied to the fields of biological medicine and cosmetics as an antibacterial component.

The present invention relates generally to the field of skin care. More specifically, the present invention relates to a cosmetic or therapeutic skin care composition comprising a combination of live bacteria of at least one strain of Propionibacterium acnes (C.acnes) and an emollient, wherein the emollient specifically supports the viability of the bacteria during storage and / or their ability to replicate after application to the skin. Preferably, the cosmetic or therapeutic skin care composition comprises bacteria of at least one strain of Propionibacterium acnes selected from D1, A5, C1, C3, H1, H2, H3, K1, K2, K4, K6, K8, K9, L1 and F4. The present invention also provides a method for treating or preventing acne by applying the skin care composition of the present invention to a skin area in need of treatment. The present invention also relates to the use of the skin care composition of the present invention for treating or preventing acne.

The application belongs to the technical field of natural plant extracts and application, and particularly relates to azafran volatile oil and application thereof in preparation of antifungal bacteriostatic products. In the application, the azafran is soaked in water and then distilled to obtain azafran volatile oil. The obtained azafran volatile oil shows good bacteriostasis, especially in inhibiting fungal strains such as Candida albicans, Malassezia furfur, Trichophyton mentagrophytes and Propionibacterium acnes. The application develops a new use of azafran volatile oil in preparation of antifungal bacteriostatic products.

The present application relates to the technical field of skin care products, in particular to a kind of acne-removing composition acting on biological membrane and its preparation method and application.By compounding Xikunshi gum and composite fermentation liquor in a certain proportion, synergistic effect can be achieved, on the one hand, the formation of propionibacterium acnes biofilm can be inhibited better, and the already formed propionibacterium acnes biofilm can also be dissolved;On the other hand, acne-removing, soothing and oil control effects can also be achieved;Preferably, Lactobacillus plantarum, Lactobacillus acidophilus and Lactobacillus reuteri are selected for composite fermentation, so that the activity of the obtained composition is better, and the effect of inhibiting acne recurrence is more significant;The present application uses reasonable active ingredient collocation and special preparation process, from the dimension of dissolving the already formed propionibacterium acnes biofilm, soothing and oil control, regulating skin microecological balance, and the comprehensive effect of inhibiting acne recurrence can be achieved, and the effect is more significant.

The invention relates to the field of cosmetics, in particular to a skin care composition containing pogostemon cablin, a preparation method of the skin care composition and cosmetics. The invention provides a skin care composition containing pogostemon cablin, which is prepared from the following components in percentage by mass: 10-25% of tea tree oil, 10-25% of pogostemon cablin, 10-25% of pogostemon cablin, 10-25% of pogostemon cablin, 10-25% of 15-30% of pogostemon cablin volatile oil; and 50 to 65% of evening primrose oil. According to the composition disclosed by the invention, all the components generate a synergistic effect, so that the inhibition effect on a propionibacterium acnes S21016 strain and staphylococcus aureus is improved; the anti-inflammatory effect is realized. The propionibacterium acnes and the staphylococcus aureus are closely related to the acnes; by inhibiting growth and reproduction of propionibacterium acnes and staphylococcus aureus bacteria, the effect of treating the acnes through bacteriostasis and sterilization is achieved.

The application belongs to the technical field of microorganisms, and discloses a propionic acid-producing propionic acid bacteria NHNK-621 and application of the propionic acid bacteria in preparation of products for improving constipation and memory, the bacteria strain has been preserved in the China Center for Type Culture Collection on November 25, 2024, and the number is CCTCC NO: M 20242631. The propionic acid bacteria NHNK-621 provided by the application has the functions of promoting 5-HT secretion, up-regulating intestinal aquaporin and brain-derived neurotrophic factor genes, metabolically producing gamma-aminobutyric acid, inhibiting growth of Candida albicans, inhibiting hypha formation of Candida albicans, combining with hypha of Candida albicans, up-regulating intestinal defensin and mucin gene expression, in addition, the bacterial biofilm can increase the colonization ability to intestinal epithelial cells, and can be used for preparing products for improving constipation and memory.

The novel invention is based, at least in part. on the surprising finding that the combination of bacterial strains comprising P. freudenreichii JS27 and B. longum subsp. infantis, particularly B. longum subsp. infantis TPY12-1 can be used in medicine and / or as a food supplement when grown simultaneously. As such and as shown in the description and appended examples, growth and viability of bacterial strains possessing synergistic behavior can be enhanced in the human gut and thus can increase bioavailability as compared with alternative combinations of bacteria.

The present invention relates to novel skin care compositions. Specifically, the present invention relates to a cosmetic or therapeutic skin care composition comprising a combination of live bacteria of at least one Propionibacterium acnes strain and an emollient that specifically supports the viability of said bacteria during storage and / or their ability to replicate upon application to the skin. Preferably, the cosmetic or therapeutic skin care composition comprises a bacterium of at least one Propionibacterium acnes strain selected from the group consisting of D1, A5, C1, C3, H1, H2, H3, K1, K2, K4, K6, K8, K9, L1 and F4. The invention also provides a method of treating or preventing acne by applying a skin care composition of the invention to an area of skin in need of treatment. The invention also relates to the use of the skin care composition of the invention for the treatment or prevention of acne.

The invention belongs to the field of biological feed, and discloses application of P.megatherium in preparation of an additive for inhibiting generation of greenhouse gas in animal waste. The P.megatherium is preserved in the Guangdong Microbial Culture Collection Center on April 18, 2025, and the preservation number is GDMCC NO: 66160. According to the present invention, the P.megatherium has efficient enzyme production capacity, and can efficiently decompose cellulose after the P.megatherium is planted in the rumen so as to significantly reduce the carbon emission of ruminants. Meanwhile, the probiotics with various enzyme production characteristics and propionibacterium are further compounded, so that the obtained intestinal probiotic composition has an obvious synergistic effect, and carbon emission is obviously reduced. Finally, a composition containing a fermentation product, an intestinal probiotic composition and a microcapsule with an insect grease slow-release function acts on the intestinal tract, and carbon emission of ruminants can be remarkably reduced.

This invention belongs to the field of bio-fermentation technology, and relates to a highly antibacterial Propionibacterium fischeri subsp. Scheres and its uses, specifically involving a strain of Propionibacterium fischeri subsp. Scheres, which is classified and named as Propionibacterium fischeri subsp. Scheres strain ( Propionibacterium freudenreichii subsp. shermanii The strain CJPF0001, with accession number CCTCC No. M 20242477, produces fermentation products with high protein content and high antibacterial activity. These products can inhibit the growth of common spoilage microorganisms, thereby improving the nutritional value of food and extending its shelf life.

The invention belongs to the technical field of bioengineering, and particularly relates to a preparation method and application of a bacillus subtilis antibacterial peptide. By accurately controlling the inoculation density culture in the logarithmic phase, the biomass density of the thalli can be increased by 2-3 times, and the fermentation period is shortened; by optimizing the composition of a fermentation culture medium, fermentation conditions and a downstream treatment method, the yield and activity of the bacillus subtilis antibacterial peptide can be remarkably improved, the broad-spectrum bacteriostasis is enhanced, the drug resistance is remarkable, and the bacillus subtilis antibacterial peptide has the tendency of selective intelligent bacteriostasis due to low concentration, namely, the bacillus subtilis antibacterial peptide has no obvious inhibition effect on skin beneficial bacteria staphylococcus epidermidis; and the traditional Chinese medicine composition has a relatively obvious inhibition effect on staphylococcus aureus, propionibacterium acnes and other strains. Moreover, only water, inorganic salt and food-grade raw materials are used in the whole process, no organic solvent is left, and compared with a traditional ethanol / acetone extraction method, the method better meets the FDA / EFSA standard.

The present invention relates generally to the field of skin care. More specifically, the present invention relates to a cosmetic or therapeutic skin care composition comprising at least one bacterial strain of the genus Staphylococcus sp. That promotes skin health, which has antimicrobial activity against bacterial strains of pathogenic Propionibacterium acnes, without affecting bacterial strains of non-pathogenic Propionibacterium acnes, and to a cosmetic or therapeutic skin care composition comprising at least one bacterial strain of the genus Staphylococcus sp. That promotes skin health, which has antimicrobial activity against bacterial strains of pathogenic Propionibacterium acnes, without affecting bacterial strains of non-pathogenic Propionibacterium acnes. The invention also provides a method of treating or preventing acne, oily skin or dry skin by applying a skin care composition of the invention to an area of skin in need of treatment. The invention also relates to the use of the skin care composition of the invention for the treatment or prevention of acne, oily skin or dry skin.