58 results about "Propionibacterium sp." patented technology

The invention relates to Penicillium decumbens C01 and application of the Penicillium decumbens C01, and belongs to the technical field of microorganisms. The penicillium decumbens C01 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the penicillium decumbens C01 is GDMCC NO.66456. The strain is obtained by separating, purifying and screening soil samples collected from botanical gardens in South China of Guangzhou; tests show that the bacteriostatic active substance generated by fermentation of the strain has a good bacteriostatic effect on staphylococcus epidermidis, staphylococcus aureus, escherichia coli, propionibacterium acnes, candida albicans and pseudomonas aeruginosa, and the bacteriostatic active substance has the characteristic of high temperature resistance and excellent preservative effect; the antibacterial preservative can be used as an antibacterial preservative raw material, is applied to the fields of food, health care products or daily chemical products and the like, and has wide application prospects and important conversion research values.

The invention belongs to the technical field of biology, and particularly relates to an antibacterial peptide for inhibiting propionibacterium acnes and application of the antibacterial peptide. The antibacterial peptide for inhibiting propionibacterium acnes is derived from marine organism hylophila, and the amino acid sequence of the antibacterial peptide is shown as SEQ ID NO.1. The amino acid sequence is novel, has remarkable antibacterial activity on propionibacterium acnes, has the advantages of low hemolysis toxicity, low cytotoxicity and high safety, and can be used for preparing the antibacterial peptide for inhibiting propionibacterium acnes. Due to the characteristics, the compound has huge application potential in the aspect of treating acnes caused by propionibacterium acnes or removing acnes.

The invention relates to the technical field of biology, in particular to a method for producing vitamin B12 through fermentation of propionibacterium shermangii, which comprises the following steps: inoculating propionibacterium shermangii into a seed culture medium to culture a seed solution; inoculating the seed solution into a fermentation culture medium for fermentation; after fermentation is finished, thalli are removed in a centrifugal separation or filtration mode, a fermentation solution is subjected to ultrafiltration concentration, a concentrated solution is purified through ion exchange chromatography or affinity chromatography, and a vitamin B12 crude product is obtained; and further purifying by adopting a crystallization method to obtain a high-purity vitamin B12 finished product. According to the method for producing the vitamin B12 through fermentation of the propionibacterium shermannii, the yield and the purity of the vitamin B12 are remarkably improved and the production cost is reduced by accurately controlling fermentation conditions, optimizing culture medium components, introducing an intelligent regulation and control system and adopting a specific purification process, so that the method is suitable for industrial production. The problems of low yield, unstable purity, difficult production process control and the like of the vitamin B12 in the prior art are solved.

The present application relates to a strain of Penicillium decumbens (P. Penicillium decumbens ) C01 and its application, and belongs to the field of microbial technology. The Penicillium decumbens C01 disclosed by the present application has been preserved in the Guangdong Microbial Digital Culture Collection Center, and its preservation number is GDMCC NO.66456. The strain is isolated, purified and screened from a soil sample collected in the garden area of Guangzhou South China Botanical Garden. Tests show that the bacteriostatic active substance produced by fermentation of the strain has good bacteriostatic effect on Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Propionibacterium acnes, Candida albicans and Pseudomonas aeruginosa. Moreover, the bacteriostatic active substance has high-temperature resistance and excellent preservative effect, and can be used as a bacteriostatic and preservative raw material, applied in the fields of food, health care products or daily chemical products, and has broad application prospect and important transformation research value.

There is provided a composition that comprises a propionic acid bacterium or a composition that comprises an oligosaccharide. The composition that comprises a propionic acid bacterium or the composition that comprises an oligosaccharide can suppress an increase of body weight of a subject by having the subject ingest the composition.

The invention discloses an oil-controlling, soothing and repairing composition and a preparation method thereof. The oil-controlling, soothing and repairing composition is prepared from a herba portulacae extract, a herba centellae extract, a baropetalum chinense leaf extract and a plankton extract. According to multiple experimental researches, the composition obtained by compounding the extracts has excellent effects of controlling oil, inhibiting activity of propionibacterium acnes, relieving and repairing, is mild and non-irritant, and has a good popularization and application prospect.

The present application relates to the field of microbial technology, and particularly relates to a gene editing method of propionibacterium acnes VB208 and application thereof. Propionibacterium acnes ) on February 20, 2023, and is preserved in the China General Microbiological Culture Collection Center, with a preservation number of CGMCC No.26576. The propionibacterium acnes screened by the present application has the characteristics of skin surface occupation, high conversion efficiency, gene modification potential and efficient expression of exogenous proteins, and can be used as an engineering strain. Furthermore, a genetic operation system suitable for the strain can be established by using the propionibacterium acnes, and the application prospect is wide.

The invention relates to hair-blacking essence and a preparation method thereof. Screening a symbiotic bacterium metabolism extract with small molecule peptide metabolism regulation capability; by combining low-concentration azelaic acid and zinc lactate components, scalp grease oxidation and abnormal proliferation of propionibacterium are inhibited; the expression of IL-10 anti-inflammatory factors is induced, the expression of TNF-alpha and IL-6 is reduced, and the hair follicle microinflammation state is reduced; a Wnt / beta-catenin pathway activator is used for inducing the melanin stem cells to be in a static state; the FGF-2 and the EGF are combined in a micro-dose manner to promote the amplification into precursor cells; mITF regulation peptides and a small amount of trace copper ions are supplemented to induce directional differentiation into mature melanocytes; a microcapsule carrier system with a three-layer structure; the penetration efficiency is improved; antioxidative peptide rich in reduced glutathione, NAD and a promoting factor are used, so that melanocytes are protected from oxidative damage; a coenzyme Q10 type mitochondrial homeostasis regulator is introduced, so that the energy metabolism is improved, the cell aging is delayed, the targeting property is strong, and the effect is accurate.

This invention discloses a strain of *Propionibacterium freundii* and its application in propionic acid production. The strain was deposited at the China Center for Type Culture Collection on October 22, 2025, with accession number CCTCC M 20252289. This invention obtains a high-propionic acid-producing *Propionibacterium freundii* strain through screening from nature. Its 16S RNA was identified as *Propionibacterium freundii*. This strain is a safe edible strain approved by the Ministry of Health. Through optimization of its culture and fermentation conditions, the final propionic acid yield can reach over 50 g / L, the glucose conversion rate can reach 60%, and there is no lactic acid accumulation, meeting the requirements of industrial production. Furthermore, through fermentation optimization, the content of other acids can be effectively controlled, resulting in propionate products of different specifications that meet market demands.

The invention relates to a propionibacterium acnes selective culture medium and a preparation method thereof, the culture medium comprises casein pancreatin digest, meat and stomach enzyme digest, heart and pancreatin digest, yeast extract powder, corn starch, sodium chloride, agar and defiberized sheep blood, and the volume is fixed with distilled water. Compared with an existing culture medium, the culture medium has the advantages of being reliable in detection and high in propionibacterium acnes separation rate and has a good application prospect.

The application relates to the technical field of biological medicine, in particular to a compound with a conjugated structure containing an alpha, beta-unsaturated aldehyde and application thereof. The compound can covalently modify Sortase A enzyme of propionibacterium acnes through alpha, beta-unsaturated aldehyde, inhibit transpeptidation catalysis and adhesion of gram-positive bacteria (for example, methicillin-resistant staphylococcus aureus, streptococcus mutans and propionibacterium acnes), block anchoring of membrane proteins, inhibit growth of gram-positive bacteria or kill bacteria; conjugated groups with different electric effects and steric effects are screened to adjust the covalent modification efficiency of alpha, beta-unsaturated aldehyde and Sortase A enzyme, strengthen the activity of the compound in inhibiting Sortase A enzyme, thereby improve the antibacterial activity of the compound, and further make the compound with the conjugated structure containing the alpha, beta-unsaturated aldehyde in the application be applied to the fields of biological medicine and cosmetics as an antibacterial component.

The application belongs to the technical field of natural plant extracts and application, and particularly relates to azafran volatile oil and application thereof in preparation of antifungal bacteriostatic products. In the application, the azafran is soaked in water and then distilled to obtain azafran volatile oil. The obtained azafran volatile oil shows good bacteriostasis, especially in inhibiting fungal strains such as Candida albicans, Malassezia furfur, Trichophyton mentagrophytes and Propionibacterium acnes. The application develops a new use of azafran volatile oil in preparation of antifungal bacteriostatic products.

The present application relates to the technical field of skin care products, in particular to a kind of acne-removing composition acting on biological membrane and its preparation method and application.By compounding Xikunshi gum and composite fermentation liquor in a certain proportion, synergistic effect can be achieved, on the one hand, the formation of propionibacterium acnes biofilm can be inhibited better, and the already formed propionibacterium acnes biofilm can also be dissolved;On the other hand, acne-removing, soothing and oil control effects can also be achieved;Preferably, Lactobacillus plantarum, Lactobacillus acidophilus and Lactobacillus reuteri are selected for composite fermentation, so that the activity of the obtained composition is better, and the effect of inhibiting acne recurrence is more significant;The present application uses reasonable active ingredient collocation and special preparation process, from the dimension of dissolving the already formed propionibacterium acnes biofilm, soothing and oil control, regulating skin microecological balance, and the comprehensive effect of inhibiting acne recurrence can be achieved, and the effect is more significant.

The invention relates to the field of cosmetics, in particular to a skin care composition containing pogostemon cablin, a preparation method of the skin care composition and cosmetics. The invention provides a skin care composition containing pogostemon cablin, which is prepared from the following components in percentage by mass: 10-25% of tea tree oil, 10-25% of pogostemon cablin, 10-25% of pogostemon cablin, 10-25% of pogostemon cablin, 10-25% of 15-30% of pogostemon cablin volatile oil; and 50 to 65% of evening primrose oil. According to the composition disclosed by the invention, all the components generate a synergistic effect, so that the inhibition effect on a propionibacterium acnes S21016 strain and staphylococcus aureus is improved; the anti-inflammatory effect is realized. The propionibacterium acnes and the staphylococcus aureus are closely related to the acnes; by inhibiting growth and reproduction of propionibacterium acnes and staphylococcus aureus bacteria, the effect of treating the acnes through bacteriostasis and sterilization is achieved.

The application belongs to the technical field of microorganisms, and discloses a propionic acid-producing propionic acid bacteria NHNK-621 and application of the propionic acid bacteria in preparation of products for improving constipation and memory, the bacteria strain has been preserved in the China Center for Type Culture Collection on November 25, 2024, and the number is CCTCC NO: M 20242631. The propionic acid bacteria NHNK-621 provided by the application has the functions of promoting 5-HT secretion, up-regulating intestinal aquaporin and brain-derived neurotrophic factor genes, metabolically producing gamma-aminobutyric acid, inhibiting growth of Candida albicans, inhibiting hypha formation of Candida albicans, combining with hypha of Candida albicans, up-regulating intestinal defensin and mucin gene expression, in addition, the bacterial biofilm can increase the colonization ability to intestinal epithelial cells, and can be used for preparing products for improving constipation and memory.

The invention belongs to the field of biological feed, and discloses application of P.megatherium in preparation of an additive for inhibiting generation of greenhouse gas in animal waste. The P.megatherium is preserved in the Guangdong Microbial Culture Collection Center on April 18, 2025, and the preservation number is GDMCC NO: 66160. According to the present invention, the P.megatherium has efficient enzyme production capacity, and can efficiently decompose cellulose after the P.megatherium is planted in the rumen so as to significantly reduce the carbon emission of ruminants. Meanwhile, the probiotics with various enzyme production characteristics and propionibacterium are further compounded, so that the obtained intestinal probiotic composition has an obvious synergistic effect, and carbon emission is obviously reduced. Finally, a composition containing a fermentation product, an intestinal probiotic composition and a microcapsule with an insect grease slow-release function acts on the intestinal tract, and carbon emission of ruminants can be remarkably reduced.

The invention belongs to the technical field of bioengineering, and particularly relates to a preparation method and application of a bacillus subtilis antibacterial peptide. By accurately controlling the inoculation density culture in the logarithmic phase, the biomass density of the thalli can be increased by 2-3 times, and the fermentation period is shortened; by optimizing the composition of a fermentation culture medium, fermentation conditions and a downstream treatment method, the yield and activity of the bacillus subtilis antibacterial peptide can be remarkably improved, the broad-spectrum bacteriostasis is enhanced, the drug resistance is remarkable, and the bacillus subtilis antibacterial peptide has the tendency of selective intelligent bacteriostasis due to low concentration, namely, the bacillus subtilis antibacterial peptide has no obvious inhibition effect on skin beneficial bacteria staphylococcus epidermidis; and the traditional Chinese medicine composition has a relatively obvious inhibition effect on staphylococcus aureus, propionibacterium acnes and other strains. Moreover, only water, inorganic salt and food-grade raw materials are used in the whole process, no organic solvent is left, and compared with a traditional ethanol / acetone extraction method, the method better meets the FDA / EFSA standard.

This invention belongs to the field of biotechnology and discloses a strain that produces vitamin B. 12 This invention relates to *Propionibacterium ferruginea* with anti-anemia function, its post-biotics, preparations, methods, and applications. The strain is named S2A35, classified as *Propionibacterium ferruginea*, with accession number CGMCC No. 37757, accession date February 9, 2026, and deposited at the China General Microbiological Culture Collection Center (CGMCC), No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. This *Propionibacterium ferruginea* strain is capable of producing active vitamin B. 12 This strain has the function of cobalamin, and both the live bacteria and its post-biotics have the function of treating anemia. It is expected to be used for vitamin B12. 12 Its production, as well as its use in preparing foods, health products, foods for special medical purposes, and medicines for the prevention, improvement, and treatment of anemia, have very broad application prospects.

The invention discloses application of propionibacterium acnes exopolysaccharide in preparation of a skin barrier function reconstruction preparation, the preparation is a pharmaceutical preparation or a cosmetic preparation, and the mass percent concentration of the propionibacterium acnes exopolysaccharide in the preparation is 0.001%-5%. According to the invention, the traditional pathogenicity cognition of the propionibacterium acnes is broken through, and the exopolysaccharides generated by fermentation of the propionibacterium acnes are creatively utilized to be applied to preparation of the skin barrier function reconstruction preparation; the composition can safely, controllably and effectively promote proliferation, differentiation and migration of keratinocytes, so that barrier reconstruction and / or repair are / is accelerated, controllable reconstruction, repair and remodeling of thinned skin due to barrier damage are realized under the condition of low dosage, and the composition has a very good application prospect.

The invention belongs to the technical field of pharmaceutical preparations and preparation and application thereof, and particularly relates to a composite antibacterial agent based on rose water and application thereof. The compound antibacterial agent based on the rose water is prepared from the following components in parts by weight: 54.1-78.9 parts of rose water, 0.2-4.0 parts of folium cortex eucommiae extract and 20.9-43.3 parts of latex matrix, the compound antibacterial agent based on the rose water has excellent antibacterial activity on staphylococcus aureus, escherichia coli and propionibacterium acnes, and the compound antibacterial agent based on the rose water has excellent antibacterial activity on staphylococcus aureus, escherichia coli and propionibacterium acnes through scientific compatibility of the latex matrix. Therefore, the preparation has the advantages of stability, easiness in smearing and natural rose fragrance.

Blepharitis, an infection and inflammation of the edge of the eyelids, and meibomian gland dysfunction (MGD), blockage of tiny oil glands at the eye lid margin, are quite common, usually under diagnosed and underappreciated diseases. There are number of causes for blepharitis, including bacterial components such as Staphylococcus aureus, Staphylococcus epidermidis, Propionibacterium acnes and Corynebacteria sp., seborrhea or microscopic mites (Demodex). More than 50% of patients seeking ophthalmologic consultations have symptoms and signs indicating one of these entities. Another large number of patients remain undiagnosed or in early stages of disease that could be easily treated thus preventing long-term pain and suffering. MGD is in fact the leading cause of dry eye disease, a potentially preventable malady.

The invention discloses a propionibacterium metabolite containing gamma-Glu-ASP and a preparation method of the propionibacterium metabolite, and belongs to the technical field of food seasonings, the propionibacterium metabolite contains 0.3% or more of gamma-Glu-ASP per unit dry weight, according to the propionibacterium metabolite containing the gamma-Glu-ASP, propionibacterium is cultured by adopting a culture medium added with two compounds of Asp and Glu, and the propionibacterium metabolite containing the gamma-Glu-ASP is prepared by utilizing the obtained propionibacterium thallus. The bacteriostatic activity of the propionibacterium metabolite containing gamma-Glu-ASP disclosed by the invention is about 50% higher than that of a conventional propionibacterium metabolite without Glu and Asp; the propionibacterium metabolite containing gamma-Glu-ASP endows food with excellent strong taste, especially has a remarkable effect on the first taste and the second taste, and can be used in the industries of seasonings or food additives and the like.

The invention discloses a propionibacterium mutant strain with high propionic acid tolerance as well as a mutation breeding method and application of the propionibacterium mutant strain, and belongs to the technical field of industrial microorganism breeding. The mutant strain is named as propionibacterium CCTCC M20252200D5-6, the propionic acid tolerance upper limit of the mutant strain reaches 85 g / L, and the propionic acid yield of the mutant strain in a 50 L fermentation tank is 76 g / L and is increased by 25.25% compared with that of a wild strain. The breeding method comprises the following steps: 1) carrying out ARTP mutagenesis (treatment is carried out for 80s, and the fatality rate is 92.2%); 2) adaptive evolution of gradient propionic acid pressure (30-80g / L); (3) carrying out primary screening on a 100g / L propionic acid flat plate; 4) carrying out targeted screening on bacterial colonies with high content of trehalose by virtue of a Raman spectrum (532nm excitation, 850cm <-1 > characteristic peak); 5) fermentation verification; the method solves the problems of low screening efficiency and poor positive mutation rate of traditional breeding, the screening flux reaches 1000 strains per day, the period is shortened to 5 days, and the method can be directly applied to industrial production of propionic acid through a microbial fermentation method and has remarkable economic and environmental benefits.

The invention discloses a probiotic and propionibacterium acnes co-culture system and application thereof. The semi-permeable membrane is arranged between the upper cavity and the lower cavity; the aperture of the semi-permeable membrane is 0.4 mu m; the upper cavity and the lower cavity comprise a liquid culture medium; the probiotics are lactobacillus plantarum and / or streptococcus thermophilus, the upper cavity is inoculated with the lactobacillus plantarum and / or streptococcus thermophilus, and the lower cavity is inoculated with propionibacterium acnes. Through culture medium, anaerobic conditions and strain proportion gradient design, stable co-culture of the three bacteria is realized, and a systematic function evaluation platform is constructed and used for probiotic screening, acne skin micro-ecological mechanism research and cosmetic functional raw material evaluation.

The invention discloses a preparation method of torreya grandis hydrolat and application of the torreya grandis hydrolat in acne improvement, and belongs to the field of plant extracts. The Chinese torreya hydrolat is obtained by performing steam distillation, condensation and oil-water separation on Chinese torreya aril to obtain a water phase and then performing filtration. The scavenging rates of DPPH and ABTS < + > free radicals are 85% and 90% or above respectively, and good oxidation resistance is achieved. Besides, antibacterial tests show that the Chinese torreya hydrolat can remarkably inhibit proliferation of propionibacterium acnes, has little influence on staphylococcus epidermidis and can be applied to cosmetics for improving acnes.

The present invention concerns a method for treating or preventing a Cutibacterium acnes bacteria-associated disease in a subject, said method comprising modulating, in particular reducing, in said subject, the ratio of the amount of C. acnes bacteria of a newly identified category called α-type to the amount of C. acnes bacteria of newly identified category called β-type, the α-type category efficiently defining acne-associated strains and the β-type defining non acne-associated strains.

Provided herein are, inter alia, compositions, systems, and methods for preventing or treating acne. Included are compositions, combinations, systems, and methods comprising at least one Propionibacterium acnes bacteriophage, at least one anti-acne compound, and a pharmaceutically acceptable carrier. Also included are compositions, combinations, and systems comprising a Propionibacterium acnes bacteriophage and an enzyme. Methods for preventing or treating acne are also provided.

A preparation and a use of a nucleic acid with anti-hair loss and oil control effects and a composition including the nucleic acid are provided. The small interfering RNA (siRNA) sequence has a significant silencing effect for the androgen receptor (AR) target gene. The composition prepared from an ionic liquid or a combination of an ionic liquid and a lipid exhibits a prominent delivery effect for water-soluble nucleic acid molecules. The composition can penetrate cells and enter cell nuclei to achieve an AR-silencing effect, and can provide significant regulatory effects for downstream target genes or proteins regulated by AR and comprehensive anti-hair loss and oil control effects. The composition exhibits a prominent transdermal permeation-promoting effect, and can permeate the epidermis layer and reach the dermis layer to exert remarkable anti-hair loss and oil control effects. In addition, the composition has a high antibacterial activity against Propionibacterium acnes.

The invention discloses a propionibacterium bacteriophage, a lyase and application of the propionibacterium bacteriophage and the lyase, particularly, the propionibacterium bacteriophage still maintains ultra-high potency (1011PFU / mL) when MOI is equal to 0.1, bacteria are almost completely removed, the treatment efficiency and safety of the bacteriophage are improved, and further, the treatment effect of the propionibacterium bacteriophage is improved. The endotoxin content of the bacteriophage disclosed by the invention is far lower than the 5EU / (kg.h) standard required in 'Consensus of Phage Treatment Clinical Application Management Experts', and the bacteriophage is harmless to a human body, does not cause serious side effects, is suitable for acne patients of different ages and health conditions, and further, has no toxic or side effects. The bacteriophage provided by the invention can also split propionibacterium acnes from all parts of a body, has broad-spectrum splitting capacity, and increases the potential application range of acne treatment and the wide inhibition capacity of the bacteriophage.