31 results about "Propionibacterium sp." patented technology

The invention belongs to the technical field of biology, and particularly relates to an antibacterial peptide for inhibiting propionibacterium acnes and application of the antibacterial peptide. The antibacterial peptide for inhibiting propionibacterium acnes is derived from marine organism hylophila, and the amino acid sequence of the antibacterial peptide is shown as SEQ ID NO.1. The amino acid sequence is novel, has remarkable antibacterial activity on propionibacterium acnes, has the advantages of low hemolysis toxicity, low cytotoxicity and high safety, and can be used for preparing the antibacterial peptide for inhibiting propionibacterium acnes. Due to the characteristics, the compound has huge application potential in the aspect of treating acnes caused by propionibacterium acnes or removing acnes.

The invention discloses an oil-controlling, soothing and repairing composition and a preparation method thereof. The oil-controlling, soothing and repairing composition is prepared from a herba portulacae extract, a herba centellae extract, a baropetalum chinense leaf extract and a plankton extract. According to multiple experimental researches, the composition obtained by compounding the extracts has excellent effects of controlling oil, inhibiting activity of propionibacterium acnes, relieving and repairing, is mild and non-irritant, and has a good popularization and application prospect.

This invention discloses a strain of *Propionibacterium freundii* and its application in propionic acid production. The strain was deposited at the China Center for Type Culture Collection on October 22, 2025, with accession number CCTCC M 20252289. This invention obtains a high-propionic acid-producing *Propionibacterium freundii* strain through screening from nature. Its 16S RNA was identified as *Propionibacterium freundii*. This strain is a safe edible strain approved by the Ministry of Health. Through optimization of its culture and fermentation conditions, the final propionic acid yield can reach over 50 g / L, the glucose conversion rate can reach 60%, and there is no lactic acid accumulation, meeting the requirements of industrial production. Furthermore, through fermentation optimization, the content of other acids can be effectively controlled, resulting in propionate products of different specifications that meet market demands.

The invention relates to a propionibacterium acnes selective culture medium and a preparation method thereof, the culture medium comprises casein pancreatin digest, meat and stomach enzyme digest, heart and pancreatin digest, yeast extract powder, corn starch, sodium chloride, agar and defiberized sheep blood, and the volume is fixed with distilled water. Compared with an existing culture medium, the culture medium has the advantages of being reliable in detection and high in propionibacterium acnes separation rate and has a good application prospect.

The application relates to the technical field of biological medicine, in particular to a compound with a conjugated structure containing an alpha, beta-unsaturated aldehyde and application thereof. The compound can covalently modify Sortase A enzyme of propionibacterium acnes through alpha, beta-unsaturated aldehyde, inhibit transpeptidation catalysis and adhesion of gram-positive bacteria (for example, methicillin-resistant staphylococcus aureus, streptococcus mutans and propionibacterium acnes), block anchoring of membrane proteins, inhibit growth of gram-positive bacteria or kill bacteria; conjugated groups with different electric effects and steric effects are screened to adjust the covalent modification efficiency of alpha, beta-unsaturated aldehyde and Sortase A enzyme, strengthen the activity of the compound in inhibiting Sortase A enzyme, thereby improve the antibacterial activity of the compound, and further make the compound with the conjugated structure containing the alpha, beta-unsaturated aldehyde in the application be applied to the fields of biological medicine and cosmetics as an antibacterial component.

The application belongs to the technical field of natural plant extracts and application, and particularly relates to azafran volatile oil and application thereof in preparation of antifungal bacteriostatic products. In the application, the azafran is soaked in water and then distilled to obtain azafran volatile oil. The obtained azafran volatile oil shows good bacteriostasis, especially in inhibiting fungal strains such as Candida albicans, Malassezia furfur, Trichophyton mentagrophytes and Propionibacterium acnes. The application develops a new use of azafran volatile oil in preparation of antifungal bacteriostatic products.

The application belongs to the technical field of microorganisms, and discloses a propionic acid-producing propionic acid bacteria NHNK-621 and application of the propionic acid bacteria in preparation of products for improving constipation and memory, the bacteria strain has been preserved in the China Center for Type Culture Collection on November 25, 2024, and the number is CCTCC NO: M 20242631. The propionic acid bacteria NHNK-621 provided by the application has the functions of promoting 5-HT secretion, up-regulating intestinal aquaporin and brain-derived neurotrophic factor genes, metabolically producing gamma-aminobutyric acid, inhibiting growth of Candida albicans, inhibiting hypha formation of Candida albicans, combining with hypha of Candida albicans, up-regulating intestinal defensin and mucin gene expression, in addition, the bacterial biofilm can increase the colonization ability to intestinal epithelial cells, and can be used for preparing products for improving constipation and memory.

The invention belongs to the field of biological feed, and discloses application of P.megatherium in preparation of an additive for inhibiting generation of greenhouse gas in animal waste. The P.megatherium is preserved in the Guangdong Microbial Culture Collection Center on April 18, 2025, and the preservation number is GDMCC NO: 66160. According to the present invention, the P.megatherium has efficient enzyme production capacity, and can efficiently decompose cellulose after the P.megatherium is planted in the rumen so as to significantly reduce the carbon emission of ruminants. Meanwhile, the probiotics with various enzyme production characteristics and propionibacterium are further compounded, so that the obtained intestinal probiotic composition has an obvious synergistic effect, and carbon emission is obviously reduced. Finally, a composition containing a fermentation product, an intestinal probiotic composition and a microcapsule with an insect grease slow-release function acts on the intestinal tract, and carbon emission of ruminants can be remarkably reduced.

This invention belongs to the field of biotechnology and discloses a strain that produces vitamin B. 12 This invention relates to *Propionibacterium ferruginea* with anti-anemia function, its post-biotics, preparations, methods, and applications. The strain is named S2A35, classified as *Propionibacterium ferruginea*, with accession number CGMCC No. 37757, accession date February 9, 2026, and deposited at the China General Microbiological Culture Collection Center (CGMCC), No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. This *Propionibacterium ferruginea* strain is capable of producing active vitamin B. 12 This strain has the function of cobalamin, and both the live bacteria and its post-biotics have the function of treating anemia. It is expected to be used for vitamin B12. 12 Its production, as well as its use in preparing foods, health products, foods for special medical purposes, and medicines for the prevention, improvement, and treatment of anemia, have very broad application prospects.

The invention discloses application of propionibacterium acnes exopolysaccharide in preparation of a skin barrier function reconstruction preparation, the preparation is a pharmaceutical preparation or a cosmetic preparation, and the mass percent concentration of the propionibacterium acnes exopolysaccharide in the preparation is 0.001%-5%. According to the invention, the traditional pathogenicity cognition of the propionibacterium acnes is broken through, and the exopolysaccharides generated by fermentation of the propionibacterium acnes are creatively utilized to be applied to preparation of the skin barrier function reconstruction preparation; the composition can safely, controllably and effectively promote proliferation, differentiation and migration of keratinocytes, so that barrier reconstruction and / or repair are / is accelerated, controllable reconstruction, repair and remodeling of thinned skin due to barrier damage are realized under the condition of low dosage, and the composition has a very good application prospect.

Blepharitis, an infection and inflammation of the edge of the eyelids, and meibomian gland dysfunction (MGD), blockage of tiny oil glands at the eye lid margin, are quite common, usually under diagnosed and underappreciated diseases. There are number of causes for blepharitis, including bacterial components such as Staphylococcus aureus, Staphylococcus epidermidis, Propionibacterium acnes and Corynebacteria sp., seborrhea or microscopic mites (Demodex). More than 50% of patients seeking ophthalmologic consultations have symptoms and signs indicating one of these entities. Another large number of patients remain undiagnosed or in early stages of disease that could be easily treated thus preventing long-term pain and suffering. MGD is in fact the leading cause of dry eye disease, a potentially preventable malady.

The invention discloses a propionibacterium mutant strain with high propionic acid tolerance as well as a mutation breeding method and application of the propionibacterium mutant strain, and belongs to the technical field of industrial microorganism breeding. The mutant strain is named as propionibacterium CCTCC M20252200D5-6, the propionic acid tolerance upper limit of the mutant strain reaches 85 g / L, and the propionic acid yield of the mutant strain in a 50 L fermentation tank is 76 g / L and is increased by 25.25% compared with that of a wild strain. The breeding method comprises the following steps: 1) carrying out ARTP mutagenesis (treatment is carried out for 80s, and the fatality rate is 92.2%); 2) adaptive evolution of gradient propionic acid pressure (30-80g / L); (3) carrying out primary screening on a 100g / L propionic acid flat plate; 4) carrying out targeted screening on bacterial colonies with high content of trehalose by virtue of a Raman spectrum (532nm excitation, 850cm <-1 > characteristic peak); 5) fermentation verification; the method solves the problems of low screening efficiency and poor positive mutation rate of traditional breeding, the screening flux reaches 1000 strains per day, the period is shortened to 5 days, and the method can be directly applied to industrial production of propionic acid through a microbial fermentation method and has remarkable economic and environmental benefits.

The invention discloses a probiotic and propionibacterium acnes co-culture system and application thereof. The semi-permeable membrane is arranged between the upper cavity and the lower cavity; the aperture of the semi-permeable membrane is 0.4 mu m; the upper cavity and the lower cavity comprise a liquid culture medium; the probiotics are lactobacillus plantarum and / or streptococcus thermophilus, the upper cavity is inoculated with the lactobacillus plantarum and / or streptococcus thermophilus, and the lower cavity is inoculated with propionibacterium acnes. Through culture medium, anaerobic conditions and strain proportion gradient design, stable co-culture of the three bacteria is realized, and a systematic function evaluation platform is constructed and used for probiotic screening, acne skin micro-ecological mechanism research and cosmetic functional raw material evaluation.

The invention discloses a preparation method of torreya grandis hydrolat and application of the torreya grandis hydrolat in acne improvement, and belongs to the field of plant extracts. The Chinese torreya hydrolat is obtained by performing steam distillation, condensation and oil-water separation on Chinese torreya aril to obtain a water phase and then performing filtration. The scavenging rates of DPPH and ABTS < + > free radicals are 85% and 90% or above respectively, and good oxidation resistance is achieved. Besides, antibacterial tests show that the Chinese torreya hydrolat can remarkably inhibit proliferation of propionibacterium acnes, has little influence on staphylococcus epidermidis and can be applied to cosmetics for improving acnes.

Provided herein are, inter alia, compositions, systems, and methods for preventing or treating acne. Included are compositions, combinations, systems, and methods comprising at least one Propionibacterium acnes bacteriophage, at least one anti-acne compound, and a pharmaceutically acceptable carrier. Also included are compositions, combinations, and systems comprising a Propionibacterium acnes bacteriophage and an enzyme. Methods for preventing or treating acne are also provided.

A preparation and a use of a nucleic acid with anti-hair loss and oil control effects and a composition including the nucleic acid are provided. The small interfering RNA (siRNA) sequence has a significant silencing effect for the androgen receptor (AR) target gene. The composition prepared from an ionic liquid or a combination of an ionic liquid and a lipid exhibits a prominent delivery effect for water-soluble nucleic acid molecules. The composition can penetrate cells and enter cell nuclei to achieve an AR-silencing effect, and can provide significant regulatory effects for downstream target genes or proteins regulated by AR and comprehensive anti-hair loss and oil control effects. The composition exhibits a prominent transdermal permeation-promoting effect, and can permeate the epidermis layer and reach the dermis layer to exert remarkable anti-hair loss and oil control effects. In addition, the composition has a high antibacterial activity against Propionibacterium acnes.

The invention belongs to the field of chemical synthesis, and relates to a production process for improving the purity of 5.6-dimethylbenzimidazole. The 2, 5, 6-dimethyl benzimidazole (5, 6-DMB) is a natural benzimidazole derivative, and is an important precursor substance for biological fermentation synthesis. The 5, 6-DMB is added into a culture medium for synthesizing the VB12 by microorganisms, so that the speed of synthesizing the VB12 by the propionibacterium can be increased. According to the present invention, the original preparation process is improved, the 4-chloro-1, 2-dimethyl benzene is adopted as the raw material, the reaction steps are shortened, the production process for improving the purity of 5.6-dimethyl benzimidazole is designed, and the important significance is provided for promoting the social development.

The invention belongs to the technical field of biological medicines, and particularly discloses a Pi scorpion polypeptide and application thereof. Through molecular biology, site-specific mutagenesis, chemical synthesis and other methods, the obtained Pi scorpion polypeptide can effectively inhibit the growth of escherichia coli, pseudomonas aeruginosa, staphylococcus aureus, propionibacterium acnes and related drug-resistant bacteria at low concentration. The pemai Pi scorpion polypeptide has a very good effect in treating or preventing folliculitis, furuncle, impetigo, acne and whelk caused by escherichia coli, pseudomonas aeruginosa, acinetobacter baumannii, propionibacterium acnes, staphylococcus aureus, related drug-resistant bacteria and the like. The pemai Pi scorpion polypeptide disclosed by the invention has good activity in resisting escherichia coli, pseudomonas aeruginosa, acinetobacter baumannii, propionibacterium acnes, staphylococcus aureus and related drug-resistant bacteria, and can be further developed and utilized as an antibacterial drug for treating folliculitis, furuncle, impetigo, acne, whelk and the like.

The invention provides an antibacterial peptide as well as a screening method and application thereof. On the first aspect, the invention provides the antibacterial peptide, and the amino acid sequence of the antibacterial peptide is as shown in SEQ ID NO. 1. The antibacterial peptide has high specificity on staphylococcus aureus in a certain concentration range, has no inhibition effect on other common skin symbiotic bacteria such as staphylococcus epidermidis, staphylococcus vorus and propionibacterium acnes, can avoid damage to normal flora balance of a human body, reduces the risk of secondary infection, and has a good application prospect. The problem of flora imbalance caused by broad-spectrum killing of the existing antibacterial peptide is solved. In addition, the minimum inhibitory concentration on staphylococcus aureus is low, the obvious bacteriostatic effect can be achieved under the condition of lower dosage, and the medicine dosage and potential side effects are reduced. In addition, the compound is derived from human skin symbiotic microorganisms and has good biocompatibility with the human body, in-vitro cytotoxicity experiments prove that the effective antibacterial concentration of the compound has no obvious toxicity on human skin keratinocytes, and the safety of the compound is superior to that of chemically synthesized antibacterial molecules.

The present application relates to the field of microorganisms, and particularly relates to a preparation method and application of a Beauveria bassiana BC-01 mycelium polysaccharide, wherein the Beauveria bassiana BC-01 has been preserved in the China General Microbiological Culture Collection Center on January 18, 2024, and the preservation number is CGMCC No. 41044. The strain produces mycelium polysaccharide with an IC 50 value of 0.5302 mg / mL for inhibiting tyrosinase; and a polysaccharide concentration of 1.5 mg / mL shows good whitening effect. The polysaccharide promotes the expression of cytokines IL-6 and TNF-alpha, and shows immune activity. The polysaccharide has the effects of whitening, antioxidation, and immune activation, and can promote the proliferation of Staphylococcus epidermidis and inhibit the proliferation of Propionibacterium acnes, and can be used as a new whitening and skin microecological material for developing cosmetics. The preparation method of the Beauveria bassiana mycelium polysaccharide is simple and easy to operate, the extraction process does not use organic reagents such as ethanol and methanol, and the production is safe and low in price.

The application belongs to the technical field of microbial fermentation, and particularly discloses a method for continuously producing propionic acid. The method inoculates the fermentation liquor of a previous batch into the fermentation medium of a next batch, realizes the recycling of propionic acid bacillus, reduces the production cost, and improves the production efficiency. After the two batches are co-fermented for a certain time, the fermentation liquor with higher activity of the next batch is inoculated into the fermentation liquor of the previous batch, the problem of low activity and slow growth of the bacteria in the late fermentation of the previous batch is solved, the fermentation period is greatly shortened, the problem of low propionic acid yield caused by feedback inhibition of fermentation products is solved, and the propionic acid yield and production rate are significantly improved.

The invention belongs to the technical field of bioengineering, and discloses a propionic acid culture medium and a propionibacterium fermentation method based on the propionic acid culture medium, and the fermentation method comprises the following preparation steps: inoculating propionibacterium into an activation culture medium, and carrying out heat preservation culture to obtain an activated strain; inoculating the activated strain into a first-stage seed culture medium, and carrying out heat preservation culture to obtain a first-stage seed; inoculating the first-stage seeds into a second-stage seed culture medium, and carrying out heat preservation culture to obtain second-stage seeds; inoculating the secondary seeds into a sterilized propionic acid culture medium containing a vitamin composition, carrying out heat preservation, stirring and fermenting, controlling the glucose concentration in the fermentation process, and detecting the propionic acid content in the fermentation process until the propionic acid content is stable and is not increased any more, so as to finish fermentation. By adopting the method, byproducts can be effectively reduced in the process of producing propionic acid through effective fermentation, and the turbidity of the suspension is prevented from rising.

A peptide exhibiting both antibacterial and anti-inflammatory activities or a derivative thereof, including an amino acid sequence of SEQ ID NO: 1 or a variant thereof. The peptides synthesized in this disclosure exhibit antibacterial and anti-inflammatory effects, inhibiting one or more of the following microbes: Escherichia coli, Staphylococcus aureus, Propionibacterium acnes, Candida albicans, as well as the expression of the inflammatory factors IL-1β, IL-6, CCL-2, and COX-2.

The invention belongs to the technical field of bacteriostatic agents and application thereof, and particularly relates to a Tibetan medicine composition, a preparation method and application of the Tibetan medicine composition in bacteriostatic products. The traditional Tibetan medicine sophora moorcroftiana seeds, cortex phellodendri, myricaria laxiflora and artemisia sieversiana are compounded, active ingredients in the traditional Tibetan medicine sophora moorcroftiana seeds, cortex phellodendri, myricaria laxiflora and artemisia sieversiana are extracted, the water-soluble plant source bacteriostatic agent with a good bacteriostatic effect is prepared, and the alkaloid content in the obtained bacteriostatic agent is 2.06-2.78 mg / g by optimizing an extraction process; in addition, the Tibetan medicine composition provided by the invention has a good inhibition effect on ten microbial bacteria such as escherichia coli, candida albicans, staphylococcus aureus, bacillus subtilis, propionibacterium acnes, streptococcus mutans, salmonella enteritidis, pseudomonas aeruginosa, porphyromonas gingivalis and staphylococcus epidermidis, and has universal antibacterial activity; the method can be widely applied to antibacterial products in various fields.

The present application relates to the technical field of external preparation preparation, and discloses a kind of nano-dispersed composition for external use prevention and treatment hair follicle related pathogenic microorganism.The composition comprises free octanoic acid 0.1-1.5%, free decanoic acid 0.1-1.5%, free lauric acid 0.1-1.5%, polyoxyethylene hydrogenated castor oil 3-10% and polyol solvent 5-20% by total weight of composition.The pH of the composition is 3.0-5.0, the particle size is 20-100 nm, and PDI≤0.3.The composition remains clear and transparent after 3 freeze-thaw cycles at-20℃ / 4℃, and exhibits synergistic antibacterial effect on hair follicle related pathogenic microorganisms such as malassezia furfur, propionibacterium acnes and staphylococcus aureus at a lower active ingredient dosage.The ternary medium-chain fatty acid nano-dispersed system of the present application has synergistic antibacterial effect and low-temperature stability, and can be used to prepare external preparations for regulating hair follicle skin microecology, inhibiting excessive proliferation of hair follicle related microorganisms or preventing and treating acne, folliculitis, malassezia related skin problems and dandruff related problems.

This invention relates to a fermenting strain of *Limosilactobacillus fermentum* LAF6 and its applications, belonging to the field of microbial technology. This strain has been deposited at the Guangdong Provincial Microbial Culture Collection Center (GDMCC NO. 66595). Safety assessments show that the bacterium has no hemolytic activity, and drug sensitivity tests show that it is sensitive to a variety of common antibiotics. Furthermore, experiments show that the fermentation broth produced by this strain has good antibacterial effects against *Burkholderia cepacia*, *Escherichia coli*, *Propionibacterium acnes*, and *Candida albicans*, while also possessing excellent antioxidant and anti-inflammatory activities. Therefore, the strain has high safety, and its fermentation broth has excellent antibacterial properties, making it a mild and low-irritant raw material suitable for the preparation of daily chemical products, with broad application prospects and significant value for translational research.

To provide a defensin expression promoter having an excellent defensin expression promoting action on the skin or in vivo.SOLUTION: Lysophosphatidic acid or its salt is used as an active ingredient. Since lysophosphatidic acid or a salt thereof has an excellent defensin expression promoting action on the skin or in vivo, it can be preferably used for the purpose of improving the antibacterial action on the skin or in vivo. It is particularly effective for promoting the expression of human β - defensin-1 and human β - defensin-3 among defensins. In addition, since lysophosphatidic acid or a salt thereof also has an action of suppressing proliferation of Propionibacterium acnes based on the action of promoting expression of human β - defensin-3, it can be used as an agent for suppressing proliferation of Propionibacterium acnes.SELECTED DRAWING: None

The application discloses a preparation method and application of hematin. The hematin is prepared from a fermentation culture of propionic acid bacteria (Corallicola sp.) SCSIO 13291 CGMCC No. 14538. The hematin is prepared from the propionic acid bacteria (Corallicola sp.) SCSIO 13291, and therefore can be applied to hematin-related drugs and food.

The present invention relates to an antibacterial and biofilm formation-inhibiting composition containing myristoleic acid as an active ingredient, wherein the myristoleic acid has been confirmed to exhibit an antibacterial effect against acne bacterium (Cutibacterium acnes) and Staphylococcus aureus, which cause skin inflammation and trouble, and to effectively inhibit the biofilms of Cutibacterium acnes and Staphylococcus aureus, at a very low concentration, and thus, the composition containing myristoleic acid as an active ingredient can be provided as an antibacterial agent and an anti-biofilm composition, and can be provided as a cosmetic composition for ameliorating skin inflammatory diseases.