57 results about "Propionibacterium sp." patented technology

The invention discloses a composition with a multi-target acne removal effect and a preparation method and application thereof. The composition comprises the following components in percentage by weight: 25%-50% of sophora flavescens extract, 25%-50% of folium artemisiae argyi extract and 10%-25% of honeysuckle extract. The inhibition of the formula of the composition on the propagation of propionibacterium acnes is embodied in a multi-target form, and the formula can ensure safety while ensuring that the formula has the effect of inhibiting acne inflammation. The sophora flavescens extract, the folium artemisiae argyi extract and the honeysuckle extract in the formula not only can inhibit propagation of propionibacterium acnes, but also can inhibit propionibacterium acnes from releasing outer membrane vesicles, and have the effect of inhibiting acne inflammation.

The invention provides application of polypeptide RK12 in preparation of a medicine for treating acne. The new application of the polypeptide RK12 is used for preparing the medicine for treating the acne, the polypeptide RK12 has a remarkable inhibition effect on propionibacterium acnes and clindamycin-resistant propionibacterium acnes, and compared with antibiotics, the polypeptide RK12 is low in drug resistance, has concentration and time dependence, and has a good application prospect in preparation of medicines for treating the acne and the clindamycin-resistant propionibacterium acnes. The research result of an in-vitro antibacterial spectrum experiment also proves that the composition can rapidly inhibit the growth of propionibacterium acnes and clindamycin-resistant propionibacterium acnes, the research result of an in-vitro anti-inflammatory experiment also proves that the composition has an obvious anti-inflammatory effect, and the composition is not easy to generate drug-resistant bacteria due to an antibacterial mechanism different from that of antibiotics. According to the new strategy and thought for treating acne provided by the invention, the antibacterial action mechanism is helpful for clinically limiting the influence of antibiotic resistance.

The invention discloses acid-resistant E.coli engineering bacteria, belonging to the field of genetic engineering. According to proteomics, secretion neighborhood ATPase is likely to be related to acid resistance of P.acidipropionici. With a molecular measure, a gene for coding secretion neighborhood ATPase in P.acidipropionici is over-expressed in E.coli BL21 (DE3) to obtain recombinant bacteria with high expression of secretion neighborhood ATPase. Compared with a contrast bacterial strain, the acid-resistant E.coli engineering bacteria have the advantage that resistance to propionic acid and acetic acid is improved to varying degrees. A research idea disclosed by the invention provides a basis and a new idea for revealing acid resisting mechanisms of propionibacterium and E.coli and improving bacterial fermentation production capability.

The present invention relates to a composition comprising one or more of bacterial strains, wherein the one or more of bacterial strains exhibit a stronger inhibition of pathogenic C. acnes strains compared to non-pathogenic C. acnes strains.

The invention discloses a short lipopeptide with specific anti-Propionibacterium acnes action and anti-inflammatory action, and belongs to the field of biotechnology. The short lipopeptide is composed of C6-C20 fatty acids and an amino acid sequence with the general formula KWKW. The short lipopeptide of the present invention can specifically inhibit the growth of Propionibacterium acnes and reduce the inflammatory response of tissues around hair follicles. In vivo and in vitro experiments show that the short lipopeptide of the present invention has high antibacterial and anti-inflammatory activities and low toxicity. In addition, the short lipopeptide has a different mechanism of action from traditional antibiotics, and the short lipopeptide is extremely difficult to produce drug resistance, especially effective for strains that have already developed resistance to existing antibiotics, so it can achieve the fundamentals of preventing and treating acne and improving the quality of life Purpose. The short lipopeptide of the present invention is a compound or complex containing lipids and amino acids, which can be added to various toiletries or cosmetics, is safe and environmentally friendly, and has broad market prospects.

The present invention proposes a method for cyclic fermentation of propionibacterium, comprising the following steps: (1) the cultured propionibacterium thallus enters a fermentation tank containing a culture medium for fermentation to obtain a fermentation liquid; (2) the step ( 1) The fermented liquid in 1) is subjected to ceramic membrane circulation filtration, and the fermented liquid supernatant liquid containing propionic acid is obtained by filtration, and the thalline concentrate liquid containing propionibacterium is sent to the fermentation tank, and fresh culture medium is added to carry out fermentation; (3) Circulation Step (2), until the acid production rate drops significantly, stop the cycle; (4) Repeat steps (1)-(3) to start a new round of cyclic fermentation of Propionibacterium. The invention adopts ceramic membrane filtration, realizes the recycling of the propionibacterium thallus, shortens the cultivation cycle of the propionibacterium thallus, reduces the production cost, and improves the production efficiency.

The invention discloses an external use type natural medicine composition for preventing and treating acne. The external use type natural medicine composition is prepared from the following componentsof herba sophorae alopecuroidis extract, herba nasturtii officinale extract and flos spiraeae ulmariae extract; or aloperine, sophoridine, herba nasturtii officinale extract, and flos spiraeae ulmariae extract. The invention also provides a preparation method of the external use type natural medicine composition. The external use type natural medicine composition has the advantages that the composition is a natural medicine extract or a single compound composition, the natural property is good, the side effect is small, the easiness in production of medicine resistance is avoided, and the like; proofed by test, the bacteria-inhibiting and anti-inflammatory functions are better, and the growth, proliferation and inflammation of propionibacterium acnes , staphylococcus epidermidis and staphylococcus aureus are inhibited; the effects of inhibiting bacteria, resisting inflammatory and inhibiting grease excretion are realized, and the irritation to skin is smaller after using for a long time; the aloperine and sophoridine components have good synergistic function, the better bacteria-inhibiting and anti-inflammatory treatment effects are realized, and the application prospect is betterin acne treatment.

The invention discloses a method for screening combined bacteria of propionibacterium and lactic acid bacteria with synergetic inhibition on activity of mold. The method comprises the following steps:(1) preparing a lactic acid bacterium seed liquid and a propionibacterium seed liquid; (2) preparing a mold spore suspension; (3) adding the two bacterial liquids (a combined bacterium) of the step (1), single bacterial liquids (single bacteria) or a blank MRS (DeMan-Rogosa-Sharpe) medium into a porous plate; and (4) adding a solid medium into each pore, after curing, adding the mold spore suspension prepared in the step (2) into each pore, performing culture and observation, comparing mold growth situations of pores with the combined bacteria and pores of single bacteria, and screening combined bacteria of propionibacterium and lactic acid bacteria with synergetic inhibition on activity of mold. The method is implemented on the porous plate, is capable of simultaneously screening multiple combined bacteria, is simple to operate, easy to implement and applicable to screening on combined bacteria of propionibacterium and lactic acid bacteria with synergetic inhibition on activity of mold.

The invention discloses an anti-dandruff cosmetic containing propionibacterium. The anti-dandruff cosmetic comprises the following raw materials in parts by weight: 0.1-0.5 parts of propionibacterium suspension, 8-30 parts of surfactant, thickener 0.2-8 parts, 0.1-5 parts of conditioning agent, 0.1-3 parts of moisturizing agent, 40-60 parts of sterile water; the concentration of the propionibacterium suspension is 0.2-4×10 2 cfu / ml; the temperature of the anti-dandruff cosmetic is 2-8°C; the pH of the anti-dandruff cosmetic is 5.5-7.3. The invention also discloses a preparation method and application method of the anti-dandruff cosmetic containing propionibacterium. Compared with the prior art, the beneficial effects of the present invention are: for the first time, propionibacterium is directly added to the anti-dandruff cosmetics, and the normal quantity of propionibacterium in the scalp can be restored after use, and the dandruff can be quickly and significantly removed without relapse, and has broad application prospects.

Provided herein are, inter alia, compositions, systems, and methods for preventing or treating acne. Included are compositions, combinations, systems, and methods comprising at least one Propionibacterium acnes bacteriophage, at least one anti-acne compound, and a pharmaceutically acceptable carrier. Also included are compositions, combinations, and systems comprising a Propionibacterium acnes bacteriophage and an enzyme. Methods for preventing or treating acne are also provided.

Provided herein are, inter alia, compositions, systems, and methods for preventing or treating acne. Included are compositions, combinations, systems, and methods comprising at least one Propionibacterium acnes bacteriophage, at least one anti-acne compound, and a pharmaceutically acceptable carrier. Also included are compositions, combinations, and systems comprising a Propionibacterium acnes bacteriophage and an enzyme. Methods for preventing or treating acne are also provided.

The invention discloses a method for producing propionibacterium bacteriocin through high-density fermentation. The method comprises the following steps: 1) performing seed cultivation; 2) performingenlargement cultivation; and 3) inoculating the seed liquid subjected to enlargement cultivation in the step (2) into a 5 L fermentation tank with a 2 L SLB culture medium according to 10 percent inoculation quantity, performing shaking table fermentation at the fermentation temperature of 30 DEG C and the shaking table rotating speed of 100 rpm, introducing CO2 during fermentation to maintain thetank pressure to be 0.05 Mpa and control the pH to be 6.0, supplementing a carbon source and a nitrogen source separately every other 24 hours starting from the 96th hour of fermentation and continuously supplementing for 5 times, wherein 100 mL of carbon source and 100 mL of nitrogen source are supplemented separately each time, the carbon source is a mixed carbon source comprising 75 percent ofsodium lactate and 25 percent of glucose, the adding concentration of the carbon source is 2 to 4 g / L, the nitrogen source comprises a yeast solution and corn vinasse clear liquid, the volume ratio of the yeast solution to the corn vinasse clear liquid is 1:(10-50), and the mass ratio of the vinasse to the distilled water in the corn vinasse clear liquid is 1:4. Due to supplementation in batches,high-density, high-yield and high-concentration cultivation of propionibacterium is guaranteed.

The present invention relates to a method and a composition to prevent or treat acne by selective inhibition of P. acnes bacteria. The method comprises treating skin with P. acnes phage-derived endolysins and nucleic acid molecules encoding the same in combination with selective essential oils.