60 results about "Propionibacterium sp." patented technology

Provided herein are ketolide derivatives, which can be used as antibacterial agents. Compounds described herein can be used for treating or preventing conditions caused by or contributed to by gram positive, gram negative or anaerobic bacteria, more particularly against, for example, Staphylococci, Streptococci, Enterococci, Haemophilus, Moraxalla spp., Chlamydia spp., Mycoplasm, Legionella spp., Mycobacterium, Helicobacter, Clostridium, Bacteroides, Corynebacterium, Bacillus, Enterobactericeae, Propionibacterium acnes or any combination thereof. Also provided are processes for preparation of compounds described herein, pharmaceutical compositions thereof, and methods of treating bacterial infections.

The invention discloses a chitosan antibacterial material modified by an antibacterial peptide as shown in SEQ ID Nos. 1 to 4. Specifically, the invention provides an antibacterial peptide-chitosan compound. Carboxyl on the antibacterial peptide and amino on the chitosan are coupled through an acylation reaction. The antibacterial peptide modified-chitosan material is obvious in antibacterial effect, and can obviously inhibit staphylococcus aureus, white staphylococcus, propionibacterium acnes or escherichia coli. Besides, the invention discloses a nanoparticle prepared from a cell growth factor loaded by the material and a preparation method of the nanoparticle. A cosmetic or a skin care product prepared by compounding the nanoparticle used as a raw material with other auxiliary materialshas the effects of suppressing acne growth and repairing the acne-damaged skin.

The invention belongs to the technical field of biological active bacterial preparations, particularly relates to a probiotic composition capable of obviously preventing and relieving the Alzheimer'sdisease, and further discloses a preparation prepared from the probiotic composition for preventing and relieving the Alzheimer's disease. The probiotic composition capable of obviously preventing andrelieving the Alzheimer's disease comprises lactobacillus plantarum Lp3a, bifidobacterium lactis Bla019, bifidobacterium longum BL5b, lactobacillus buchneri Lbu3, propionibacterium freudenreichii PrF01, propionibacterium shermanii, pediococcus acidilactici, clostridium butyricum and lactobacillus fermenti. The probiotic composition not only can obviously regulate and maintain intestinal microecology and control an in vivo toxin level but also can generate a certain quantity of short chain fatty acid, including acetic acid, propionic acid, butyric acid, lactic acid and the like to further relieve the related symptoms of the Alzheimer's disease. The probiotic composition has the efficacy of alleviating and relieving the symptoms of the Alzheimer's disease, also has the efficacy of soothingthe nerves and calming down, and also has a certain function for the depression, the anxiety disorder and the insomnia.

The invention concerns an in vitro method for determining if an individual is infected by a bacterium of the Propionibacterium genus comprising: (i) detecting antibodies directed against at least one protein of sequence SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6 or SEQ ID NO: 8, in a biological sample of the individual, and (ii) deducing therefrom that the individual is infected by a bacterium of the Propionibacterium genus. The invention further concerns the kit for diagnosing of such an infection.

The invention discloses a cheese and the method for preparing the same. The methbod comprises following steps: adding thermo-streptococcus, lactobacillus bulgaricus, propionibaterium shermanii and plant oil emulsion into raw milk, culturing 20-60 minutes at 30 -39 Deg. C; adding chymosin to form curd; preparing cheese with curd. The cheeses is characterized by high CLA content, soft taste, light scent, even porosity distribution, proper hardness, good plastic property and lacte color.

The invention discloses a composition with a multi-target acne removal effect and a preparation method and application thereof. The composition comprises the following components in percentage by weight: 25%-50% of sophora flavescens extract, 25%-50% of folium artemisiae argyi extract and 10%-25% of honeysuckle extract. The inhibition of the formula of the composition on the propagation of propionibacterium acnes is embodied in a multi-target form, and the formula can ensure safety while ensuring that the formula has the effect of inhibiting acne inflammation. The sophora flavescens extract, the folium artemisiae argyi extract and the honeysuckle extract in the formula not only can inhibit propagation of propionibacterium acnes, but also can inhibit propionibacterium acnes from releasing outer membrane vesicles, and have the effect of inhibiting acne inflammation.

The invention provides recombinant mortierella alpina oPAI for heterologous expression of linoleic acid isomerase gene from propionibacterium acnes and provides a method for constructing a recombinant mortierella alpina strain for heterologous expression of linoleic acid isomerase gene from propionibacterium acnes, and the invention further provides a use of the recombinant mortierella alpina strain for producing fatty acid, namely biological synthesizing conjugated linoleic acid. With mortierella alpina uracil auxotroph strain MAU1 as a material, a recombinant strain for heterologous expression of linoleic acid isomerase gene from propionibacterium acnes is successfully constructed, and a target product conjugated linoleic acid is detected in the recombinant strain added with long-chain acyl-coenzyme A for synthesizing an enzyme inhibitor.

The invention belongs to the technical field of feed mildew preventives and provides a method for producing a propionate mildew preventive through microbial fermentation and a use thereof. Through liquid fermentation, propionibacterium can produce propionate. The method comprises carrying out seed enlarged cultivation on propionibacterium, preparing a liquid propionibacterium strain, inoculating a liquid culture medium with the liquid propionibacterium strain, carrying out fermentation to obtain propionic acid, wherein in fermentation, alkali solution fed-batch is carried out so that the propionic acid and alkali undergo a reaction to produce a propionate, stopping fermentation when the propionate content is 18-25 g / L or g / kg, filtering the fermentation solution through a filter press, taking the filtrate, feeding the filtrate into a spray tower, and carrying out spray drying to obtain powder as a propionate product. The method needs less equipment, has simple processes and can replace the chemical synthesis method.

The invention provides application of polypeptide RK12 in preparation of a medicine for treating acne. The new application of the polypeptide RK12 is used for preparing the medicine for treating the acne, the polypeptide RK12 has a remarkable inhibition effect on propionibacterium acnes and clindamycin-resistant propionibacterium acnes, and compared with antibiotics, the polypeptide RK12 is low in drug resistance, has concentration and time dependence, and has a good application prospect in preparation of medicines for treating the acne and the clindamycin-resistant propionibacterium acnes. The research result of an in-vitro antibacterial spectrum experiment also proves that the composition can rapidly inhibit the growth of propionibacterium acnes and clindamycin-resistant propionibacterium acnes, the research result of an in-vitro anti-inflammatory experiment also proves that the composition has an obvious anti-inflammatory effect, and the composition is not easy to generate drug-resistant bacteria due to an antibacterial mechanism different from that of antibiotics. According to the new strategy and thought for treating acne provided by the invention, the antibacterial action mechanism is helpful for clinically limiting the influence of antibiotic resistance.

The invention discloses an external use type natural medicine composition for preventing and treating acne. The external use type natural medicine composition is prepared from the following componentsof herba sophorae alopecuroidis extract, herba nasturtii officinale extract and flos spiraeae ulmariae extract; or aloperine, sophoridine, herba nasturtii officinale extract, and flos spiraeae ulmariae extract. The invention also provides a preparation method of the external use type natural medicine composition. The external use type natural medicine composition has the advantages that the composition is a natural medicine extract or a single compound composition, the natural property is good, the side effect is small, the easiness in production of medicine resistance is avoided, and the like; proofed by test, the bacteria-inhibiting and anti-inflammatory functions are better, and the growth, proliferation and inflammation of propionibacterium acnes , staphylococcus epidermidis and staphylococcus aureus are inhibited; the effects of inhibiting bacteria, resisting inflammatory and inhibiting grease excretion are realized, and the irritation to skin is smaller after using for a long time; the aloperine and sophoridine components have good synergistic function, the better bacteria-inhibiting and anti-inflammatory treatment effects are realized, and the application prospect is betterin acne treatment.

The invention relates to a slow-releasing agent or rslow-releasing implanting agent containing antibiotics. The injection comprises slow-releasing micro-sphere and dissolvent. The slow-releasing micro-sphere contains slow-releasing findings and antibiotics, and dissolvent contains suspending adjuvant agent such as sodium carboxymethyl cellulose with the adhesive degree being 100cp-3000cp (at 20-30 Deg. C). The slow-releasing findings comprises EVAc, polyphenyl, PLA, PLGA, decanedioic acid copolymer, albumen glue and gelatin; the slow-releasing implanting agent is prepared with slow-releasing micro-sphere or through other methods. When said antibiotics is placed or injected into bacteria, the medicine can be released slowly for more than 10 days, and toxicity is dramatically reduced at the same time when effective medicine concentration is got and maintained. The said slow-releasing agent is specially effective for chronic medullitis, bedsore, intractable ulcer on skin, diabetes femoral head necrosis and other abscessus, which are caused by Staphylococcus, Streptococcus, Streptococcus, acne Propionibacteriaceae, Enterobacter, Enterobacter, gonotoxin or parameningococcus.

The invention relates to the field of polypeptide drugs, specifically to a Bombina orientalis polypeptide, and a preparation method and application thereof. The Bombina orientalis polypeptide ABP27 has an amino acid sequence as shown in SEQ ID No. 1. Experimental results show that the ABP27 has good antibacterial activity to Staphylococcus aureus, Escherichia coli DH5alpha, Bacillus acnes, animal pathogenic Escherichia coli, Candida albicans and Saccharomyces sake. Moreover, the ABP27 has good corrosion resistance and can used as an antiseptic substitute. The ABP27 is capable of inhibiting inflammatory response of Propionibacterium acnes. The Bombina orientalis polypeptide ABP27 provided by the invention can be used for preparing antibacterial drugs or cosmetics, has a wide application scope and produces good economic and social effect.

The invention discloses acid-resistant E.coli engineering bacteria, belonging to the field of genetic engineering. According to proteomics, secretion neighborhood ATPase is likely to be related to acid resistance of P.acidipropionici. With a molecular measure, a gene for coding secretion neighborhood ATPase in P.acidipropionici is over-expressed in E.coli BL21 (DE3) to obtain recombinant bacteria with high expression of secretion neighborhood ATPase. Compared with a contrast bacterial strain, the acid-resistant E.coli engineering bacteria have the advantage that resistance to propionic acid and acetic acid is improved to varying degrees. A research idea disclosed by the invention provides a basis and a new idea for revealing acid resisting mechanisms of propionibacterium and E.coli and improving bacterial fermentation production capability.

The invention discloses an anti-acne fucoxanthin-agar oligosaccharide composition, a preparation method and application of the composition. The anti-acne fucoxanthin-agar oligosaccharide composition contains agar oligosaccharides, fucoxanthin, plant glycosides, surfactants, stabilizers and water. The growth of two acne pathogenic bacteria, Propionibacterium acnes and Staphylococcus aureus, can beinhibited by the composition provided by the invention, and the development of acne conditions can be effectively controlled.

Bacteriophage compositions and therapeutic uses thereof. In particular, compositions of lytic bacteriophages that are capable of lysing Propionibacterium acnes (P. acnes) bacterial strains associated with acne and biofilms, thereby treating or preventing acne and biofilms.

The invention provides lactic acid bacteria mask essence capable of inhibiting propionibacterium acnes and a preparation method. The lactic acid bacteria mask essence is prepared from 5 parts of propylene glycol, 2 parts of PEG-40 hydrogenated castor oil, 1.4 parts of sodium hyaluronate, 2 parts of dipotassium glycyrrhetate, 0.2 part of carbomer, 0.1 part of hydroxyethyl cellulose, 0.2 part of chlorphenesin, 0.05 part of pelargonium graveolens oil, 0.2 part of phenoxyethanol, 2.1 parts of triethanolamine, 0.5 part of inulin, 0.84 part of inactivated freeze-dried powder, and the balance of water. For the lactic acid bacteria mask essence capable of removing acnes provided by the invention, the experiment proves that the lactic acid bacteria mask essence has an obvious inhibition effect on propionibacterium acnes, the diameter of an inhibition zone can reach 17.94 mM, meanwhile, the lactic acid bacteria mask essence is not sensitive to temperature and illumination and has good stabilityunder the room temperature condition and natural illumination, and the change of the diameter of the inhibition zone is less than 2mM after the lactic acid bacteria mask essence is stored for two months under the conditions of room temperature and natural illumination.

The present invention relates to a novel quinolonecarboxylic acid derivatives represented by general formula which have potent antibacterial effects on gram-positive bacteria, in particular, Propionibacterium acnes, wherein R<1> represents hydrogen or a carboxyl-protective group; R<2> represents optionally substituted cycloalkyl; R<3> represents hydrogen, halogeno, optionally substituted alkyl, alkoxy or alkylthio, optionally protected hydroxy or amino, or nitro; R<4> represents optionally substituted alkyl or alkoxy; Z represents halogeno, optionally substituted alkyl, alkenyl, cycloalkyl, alkoxy, alkylthio, amino or pyridin-4-yl or pyridin-3-yl optionally having one or more substituents selected from among optionally substituted hydroxy and amino.

The invention belongs to the field of biomedicine and specifically relates to an anti-acne composition and a preparation method thereof. the anti-acne composition comprises the following raw materials(by weight): 100-140 parts of camellia sinensis essential oil, 140-180 parts of magnolia cortex essential oil, 100-140 parts of magnolol, 80-120 parts of honokiol, 60-100 parts of oleum menthae piperitae, 80-120 parts of chinese mosla herb essential oil, 40-80 parts of Helichrysum Italicum essential oil and 30-70 parts of Oleum Ocimi Gratissimi. The anti-acne composition effectively inhibits multiplication of bacteria such as propionibacterium acnes, etc., to realize treatment and prevention of acne. The anti-acne composition of the scheme can be applied to research and development and preparation of anti-acne products.

The invention discloses a short lipopeptide with specific anti-Propionibacterium acnes action and anti-inflammatory action, and belongs to the field of biotechnology. The short lipopeptide is composed of C6-C20 fatty acids and an amino acid sequence with the general formula KWKW. The short lipopeptide of the present invention can specifically inhibit the growth of Propionibacterium acnes and reduce the inflammatory response of tissues around hair follicles. In vivo and in vitro experiments show that the short lipopeptide of the present invention has high antibacterial and anti-inflammatory activities and low toxicity. In addition, the short lipopeptide has a different mechanism of action from traditional antibiotics, and the short lipopeptide is extremely difficult to produce drug resistance, especially effective for strains that have already developed resistance to existing antibiotics, so it can achieve the fundamentals of preventing and treating acne and improving the quality of life Purpose. The short lipopeptide of the present invention is a compound or complex containing lipids and amino acids, which can be added to various toiletries or cosmetics, is safe and environmentally friendly, and has broad market prospects.

The present invention proposes a method for cyclic fermentation of propionibacterium, comprising the following steps: (1) the cultured propionibacterium thallus enters a fermentation tank containing a culture medium for fermentation to obtain a fermentation liquid; (2) the step ( 1) The fermented liquid in 1) is subjected to ceramic membrane circulation filtration, and the fermented liquid supernatant liquid containing propionic acid is obtained by filtration, and the thalline concentrate liquid containing propionibacterium is sent to the fermentation tank, and fresh culture medium is added to carry out fermentation; (3) Circulation Step (2), until the acid production rate drops significantly, stop the cycle; (4) Repeat steps (1)-(3) to start a new round of cyclic fermentation of Propionibacterium. The invention adopts ceramic membrane filtration, realizes the recycling of the propionibacterium thallus, shortens the cultivation cycle of the propionibacterium thallus, reduces the production cost, and improves the production efficiency.

The invention discloses a Propionibacterium jensenii strain and application thereof. The Propionibacterium jensenii strain is preserved in the China Center for Type Culture Collection on May 3, 2012, and has a preservation number of CCTCC No.M 2012153. The Propionibacterium jensenii is screened out from sewage sludge from a cheese factory; and yield of propionic acid produced from fermentation bythe Propionibacterium jensenii reaches 20.96g / L.

The invention discloses an external use type natural medicine composition for preventing and treating acne. The external use type natural medicine composition is prepared from the following componentsof herba sophorae alopecuroidis extract, herba nasturtii officinale extract and flos spiraeae ulmariae extract; or aloperine, sophoridine, herba nasturtii officinale extract, and flos spiraeae ulmariae extract. The invention also provides a preparation method of the external use type natural medicine composition. The external use type natural medicine composition has the advantages that the composition is a natural medicine extract or a single compound composition, the natural property is good, the side effect is small, the easiness in production of medicine resistance is avoided, and the like; proofed by test, the bacteria-inhibiting and anti-inflammatory functions are better, and the growth, proliferation and inflammation of propionibacterium acnes , staphylococcus epidermidis and staphylococcus aureus are inhibited; the effects of inhibiting bacteria, resisting inflammatory and inhibiting grease excretion are realized, and the irritation to skin is smaller after using for a long time; the aloperine and sophoridine components have good synergistic function, the better bacteria-inhibiting and anti-inflammatory treatment effects are realized, and the application prospect is betterin acne treatment.

The invention discloses a method for screening combined bacteria of propionibacterium and lactic acid bacteria with synergetic inhibition on activity of mold. The method comprises the following steps:(1) preparing a lactic acid bacterium seed liquid and a propionibacterium seed liquid; (2) preparing a mold spore suspension; (3) adding the two bacterial liquids (a combined bacterium) of the step (1), single bacterial liquids (single bacteria) or a blank MRS (DeMan-Rogosa-Sharpe) medium into a porous plate; and (4) adding a solid medium into each pore, after curing, adding the mold spore suspension prepared in the step (2) into each pore, performing culture and observation, comparing mold growth situations of pores with the combined bacteria and pores of single bacteria, and screening combined bacteria of propionibacterium and lactic acid bacteria with synergetic inhibition on activity of mold. The method is implemented on the porous plate, is capable of simultaneously screening multiple combined bacteria, is simple to operate, easy to implement and applicable to screening on combined bacteria of propionibacterium and lactic acid bacteria with synergetic inhibition on activity of mold.

The present invention discloses an application of sophorolipid in preparation of a medicine for preventing against propionibacterium acnes and a biofilm thereof. The sophorolipid is prepared from Wickerhamiella domercqiae var. sophorollipid CGMCC No.1576 through a fermentation method; a concentration of the sophorolipid in effectively inhibiting propionibacterium acnes is 0.12-1.5 g / L and a concentration of the sophorolipid in effectively inhibiting propionibacterium acnes biofilm is 2.0-2.5 g / L. Experiments confirm that the sophorolipid has a significant bacteriostatic effect, does not have any toxic and side effects on human body, can be used to replace a conventional antibiotic medicine that is prone to enable people to relapse and infect and have drug resistance after therapy in certain aspects, and has great application prospects.

The invention discloses dandruff removing cosmetic containing propionibacterium. The dandruff removing cosmetic is prepared from, by weight, 0.1-0.5 part of propionibacterium suspension, 8-30 parts of surfactant, 0.2-8 parts of thickener, 0.1-5 parts of conditioner, 0.1-3 parts of humectants and 40-60 parts of sterile water. The concentration of the propionibacterium suspension is (0.2-4)*10<2> cfu / ml, the temperature of the dandruff removing cosmetic is 2-8 DEG C, and the pH value of the dandruff removing cosmetic is 5.5-7.3. The invention further discloses a preparing method and a using method of the dandruff removing cosmetic containing propionibacterium. Compared with the prior art, the dandruff removing cosmetic has the advantages that propionibacterium is directly added into the dandruff removing cosmetic, the normal number of propionibacterium in the scalp can be recovered after using the dandruff removing cosmetic, dandruff is removed fast and remarkably and does not relapse, and the dandruff removing cosmetic has broad application prospects.

Provided herein are, inter alia, compositions, systems, and methods for preventing or treating acne. Included are compositions, combinations, systems, and methods comprising at least one Propionibacterium acnes bacteriophage, at least one anti-acne compound, and a pharmaceutically acceptable carrier. Also included are compositions, combinations, and systems comprising a Propionibacterium acnes bacteriophage and an enzyme. Methods for preventing or treating acne are also provided.

Provided is a composition that contains propionic acid bacteria or oligosaccharide. An increase in body mass can be inhibited by ingestion, by a subject, of the composition that contains propionic acid bacteria or oligosaccharide.

The invention provides a composition for repairing micro-ecological balance of skin, a care bath lotion and a preparation method of the care bath lotion. The composition is mainly composed of hexine dihydroxy sulfonate and bacillus licheniformis glutamic acid fermentation product filtrate, the care bath lotion containing the composition can inhibit reproduction of skin harmful bacteria such as staphylococcus aureus and propionibacterium acnes and promote reproduction of beneficial bacteria such as staphylococcus epidermidis, so that the problems of skin itch, scurf and the like caused by the fact that traditional bath lotion damages skin micro-ecological balance are solved.