A natural plant extract composition for preventing hair loss and strengthening hair, and its preparation method and application

Abstract

The present invention discloses a natural plant extract composition for preventing hair loss and fixing hair. Through the reasonable proportion and synergistic effect of seven kinds of plant extracts, including Hypericum perforatum extract, Guaiacaceae extract, Mangostana mangosteen peel extract, mango leaf extract, Polygonum multiflorum extract, Platycladus orientalis leaf extract, and ginger extract, 5α-reductase and main microorganisms such as Malassezia furfur, Propionibacterium acnes and Staphylococcus aureus that cause seborrheic alopecia are effectively inhibited, thereby reducing the probability of seborrheic alopecia. The beneficial ingredients in the seven kinds of plant extracts have the effects of promoting blood circulation and regulating scalp oil secretion, can promote hair growth, and improve the scalp environment. In addition, mango leaf extract and Polygonum multiflorum extract are rich in vitamins and nutrients, can nourish hair, enhance the elasticity and gloss of hair, and reduce hair breakage and shedding. The present invention also discloses the preparation method and application of the above-mentioned natural plant extract composition for preventing hair loss and fixing hair.

Description

Technical Field

[0001] The invention relates to the technical field of daily necessities, and in particular to a natural plant extract composition for preventing hair loss and strengthening hair, and a preparation method and application thereof. Background Art

[0002] Hair loss is a common health problem that not only affects appearance, but can also have a negative impact on a person's mental health and self-confidence. Hair loss is usually caused by a variety of factors, including genetic factors, hormonal changes, lifestyle, environmental factors, and specific health problems. There are many common types of hair loss, such as seborrheic alopecia, psychogenic alopecia, physical alopecia, chemical alopecia, etc. According to statistics, seborrheic alopecia accounts for more than 90% of all types of hair loss. Seborrheic alopecia refers to the phenomenon that excessive secretion of oil from the scalp leads to greasy hair, clogged hair follicles and inflammation, which eventually causes hair loss.

[0003] The main cause of seborrheic alopecia is abnormal oil secretion, which causes blockage of hair follicles. Excess oil will block the opening of hair follicles and hinder hair growth. Excess oil can easily breed bacteria, leading to scalp inflammation, which further affects hair growth. The main prevention and treatment methods are: (1) Use appropriate shampoo and hair care products to clean the scalp and control oil secretion; (2) Drug treatment: including topical drugs (such as minoxidil, ketoconazole, etc.) and oral drugs (such as vitamins, zinc, etc.) to regulate oil secretion and improve scalp health; (3) Improve lifestyle habits: Reducing stress, maintaining good eating habits, and regular work and rest can help improve seborrheic alopecia.

[0004] There are many anti-hair loss products on the current market, but some of them also have many defects, such as: using Western medicine, irritating, and uncertain efficacy. For example, minoxidil is a common drug that promotes hair growth. The drug must be taken twice a day without interruption. Once it is discontinued, hair loss will recur. Therefore, products based on natural plant extracts are more popular. Some of these products mainly use the blood circulation function of ginger extract to promote blood circulation in the head skin through ginger extract to reduce hair loss. Some products are based on traditional Chinese medicine theory and use compound herbal extracts. Depending on the different formula raw materials, they produce nourishing or degreasing effects to prevent hair loss. However, from the research and analysis of listed products, the effects of these types of anti-hair loss products are not very obvious. Therefore, it is necessary to provide a combination product with higher safety and better therapeutic effect. Summary of the invention

[0005] In view of this, the present invention proposes a natural plant extract composition for preventing hair loss and strengthening hair, and a preparation method and application thereof, to solve the above problems.

[0006] The technical solution of the present invention is achieved in this way:

[0007] A natural plant extract composition for preventing hair loss and strengthening hair, comprising, by weight: 25-45 parts of Hypericum perforatum extract, 20-35 parts of Guaiacum jasminoides extract, 15-30 parts of Mangosteen fruit peel extract, 10-30 parts of mango leaf extract, 10-25 parts of Polygonum multiflorum extract, 8-15 parts of Platycladus orientalis leaf extract, and 6-12 parts of ginger extract.

[0008] Preferably, the natural plant extract composition for preventing hair loss and strengthening hair comprises, by weight: 30-40 parts of Hypericum perforatum extract, 25-30 parts of Guaiac Wood extract, 20-30 parts of Mangosteen fruit peel extract, 15-25 parts of mango leaf extract, 15-20 parts of Polygonum multiflorum extract, 10-12 parts of Platycladus orientalis leaf extract, and 8-10 parts of ginger extract.

[0009] Hypericum perforatum extract is a product extracted from the flowering tips or aerial parts of Hypericum Perforatum L. as raw materials. The main component is hypericum (Mycoporphyrin), which belongs to the dianthrone compound and is the most biologically active substance in Hypericum perforatum. Its chemical formula is C30H16O8.

[0010] Guaiacum officinale extract is an extract of the plant of the genus Guaiacum (Guaiacum officinale L.) of the Tribulus family. The main chemical components of the guaiacum extract include brunetol, guaiacol, β-eudesmol, α-brunetene, γ-eudesmol and the like.

[0011] Mangosteen peel extract, also known as mangosteen peel extract, is an extract from the peel of Garcinia multiflora Champ. and G. obl. of the Garcinia family. The main components are α-mangosteen, hydroxycitric acid and xanthones.

[0012] Mango leaf extract is an extract of the leaves of the plant mango Mangiferaindica L. of the Anacardiaceae family. According to the Chinese Medicine Dictionary, mango leaves are sour, sweet, and cool in nature. They can promote the flow of qi and relieve stagnation, remove sha accumulation, treat abdominal pain caused by heat stagnation, and flatulence, and wash sores. The active ingredient of mango leaf extract is mangiferin.

[0013] Polygonum multiflorum extract is the dried root tuber of Polygonum multiflorum, a plant of the Polygonaceae family. Its main functional ingredients are lecithin, rhein, and rhein.

[0014] Platycladus orientalis leaf extract is from the branches and leaves of Platycladus orientalis (L.) Franco, a plant of the Cupressaceae family. It has the effects of cooling blood and stopping bleeding, promoting hair growth and darkening hair.

[0015] Ginger extract is an extract of the underground rhizome of Zingiber officinale, containing volatile oils and gingerols, among which gingerols include: gingerols, gingerediols, gingerols, gingerones, and gingerdones.

[0016] The present invention also provides a method for preparing a natural plant extract composition for preventing hair loss and strengthening hair, comprising the following steps:

[0017] S1. Prepare Hypericum perforatum flower, guaiac wood, mangosteen peel, mango leaf, Polygonum multiflorum root, Platycladus orientalis leaf, and ginger, wash, dry, crush, mix with alcohol solvents respectively, extract at 100-120° C., collect the extract, concentrate, and dry to obtain the corresponding Hypericum perforatum extract, guaiac wood extract, mangosteen peel extract, mango leaf extract, Polygonum multiflorum extract, Platycladus orientalis leaf extract, and ginger extract;

[0018] S2. Stir and mix Hypericum perforatum extract, Guaiac Wood extract, Mangosteen fruit peel extract, mango leaf extract, Polygonum multiflorum extract, Platycladus orientalis leaf extract, and ginger extract according to weight portions, and homogenize at 30-40°C. After homogenization, stir continuously for 2-3 hours, take out, and obtain a natural plant extract composition for preventing hair loss and strengthening hair.

[0019] Preferably, the alcohol solvent includes anhydrous ethanol; the mass volume g / mL ratio of the Hypericum perforatum flower, guaiac wood, mangosteen peel, mango leaf, Polygonum multiflorum root, Platycladus orientalis leaf, ginger and anhydrous ethanol is 1:15-20.

[0020] The present invention also provides an application of the natural plant extract composition for preventing hair loss and strengthening hair as an additive for hair care products.

[0021] The present invention also provides an application of a natural plant extract composition for preventing hair loss and strengthening hair in the preparation of hair care products.

[0022] The present invention also provides a hair care product, comprising the natural plant extract composition for preventing hair loss and strengthening hair, and auxiliary materials acceptable to the hair care product.

[0023] Preferably, the auxiliary materials include one or more of a solubilizing agent, a penetration enhancer, a surfactant, a thickening agent and a preservative.

[0024] Preferably, the solvent may be one or more of water, ethanol and propanol.

[0025] Preferably, the penetration enhancer may be one or more of hexylene glycol, butanediol, and polyethylene glycol.

[0026] Preferably, the surfactant may be one or more of sodium laureth sulfate, sodium dodecyl sulfate, cocamide, and lauroyl hydroxysulfobetaine.

[0027] Preferably, the thickener can be one or more of hexylene glycol, hydroxypropyl methylcellulose, xanthan gum.

[0028] Preferably, the preservative may be one or more of benzoic acid, parabens, and phenoxyethanol.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] The present invention provides a natural plant extract composition for preventing hair loss and strengthening hair. Through the synergistic effect of seven ingredients, namely, Hypericum perforatum extract, Guaiacum chinense extract, Mangosteen peel extract, Mangosteen leaf extract, Polygonum multiflorum extract, Platycladus orientalis leaf extract, and Ginger extract, the composition has a high inhibitory effect on 5α-reductase and major microorganisms such as Malassezia furfur, Propionibacterium acnes, and Staphylococcus aureus that cause seborrheic alopecia, thereby reducing the probability of seborrheic alopecia. Among them, Hypericum perforatum extract, Polygonum multiflorum extract, and Ginger extract have the effect of promoting blood circulation, which can increase the blood flow of scalp capillaries, help hair follicles absorb more nutrients, and promote hair growth; Guaiacum chinense extract, Mangosteen peel extract, and Platycladus orientalis leaf extract have the effect of anti-inflammatory and soothing scalp, which can reduce the inflammatory response of the scalp and improve the scalp environment; Mangosteen leaf extract and Polygonum multiflorum extract are rich in vitamins and nutrients, which can nourish hair, enhance the elasticity and luster of hair, and reduce hair breakage and shedding. DETAILED DESCRIPTION

[0031] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0032] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0033] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0034] Example 1

[0035] A natural plant extract composition for preventing hair loss and strengthening hair, comprising, by weight: 25 parts of Hypericum perforatum extract, 20 parts of Guaiacum jasminoides extract, 15 parts of Mangosteen fruit peel extract, 10 parts of mango leaf extract, 10 parts of Polygonum multiflorum extract, 8 parts of Platycladus orientalis leaf extract, and 6 parts of ginger extract.

[0036] The method for preparing the natural plant extract composition for preventing hair loss and strengthening hair comprises the following steps:

[0037] S1. Prepare Hypericum perforatum flower, guaiac wood, mangosteen peel, mango leaf, Polygonum multiflorum root, Platycladus orientalis leaf, and ginger, wash and dry them, crush them, and mix them with anhydrous ethanol respectively, wherein the mass volume g / mL ratio of the Hypericum perforatum flower, guaiac wood, mangosteen peel, mango leaf, Polygonum multiflorum root, Platycladus orientalis leaf, ginger and anhydrous ethanol is 1:15, reflux extraction is performed 3 times at 100°C, each time for 3 hours, and the three filtrates are combined after filtration, and the solvent is recovered by concentration under reduced pressure to obtain a concentrated solution, which is placed in an oven at 40°C for drying to obtain the corresponding Hypericum perforatum extract, guaiac wood extract, mangosteen peel extract, mango leaf extract, Polygonum multiflorum extract, Platycladus orientalis leaf extract, and ginger extract;

[0038] S2. Stir and mix Hypericum perforatum extract, Guaiacum guaiac extract, Mangosteen fruit peel extract, mango leaf extract, Polygonum multiflorum extract, Platycladus orientalis leaf extract, and ginger extract according to weight portions, and homogenize at 30°C at a homogenizing speed of 2000 rpm for 30 min. After the homogenization, stir continuously for 2 h, take out, and obtain a natural plant extract composition for preventing hair loss and strengthening hair.

[0039] Example 2

[0040] A natural plant extract composition for preventing hair loss and strengthening hair, comprising, by weight: 30 parts of Hypericum perforatum extract, 25 parts of Guaiacum jasminoides extract, 20 parts of Mangosteen fruit peel extract, 15 parts of mango leaf extract, 15 parts of Polygonum multiflorum extract, 10 parts of Platycladus orientalis leaf extract, and 8 parts of ginger extract.

[0041] The method for preparing the natural plant extract composition for preventing hair loss and strengthening hair comprises the following steps:

[0042] S1. Prepare Hypericum perforatum flower, guaiac wood, mangosteen peel, mango leaf, Polygonum multiflorum root, Platycladus orientalis leaf, and ginger, wash and dry, crush, and mix with anhydrous ethanol respectively, wherein the mass volume g / mL ratio of the Hypericum perforatum flower, guaiac wood, mangosteen peel, mango leaf, Polygonum multiflorum root, Platycladus orientalis leaf, ginger and anhydrous ethanol is 1:20, reflux extraction is performed at 110°C for 3 times, each time for 3 hours, and the three filtrates are combined after filtration, and the solvent is recovered by concentration under reduced pressure to obtain a concentrated solution, which is placed in an oven at 40°C for drying to obtain the corresponding Hypericum perforatum extract, guaiac wood extract, mangosteen peel extract, mango leaf extract, Polygonum multiflorum extract, Platycladus orientalis leaf extract, and ginger extract;

[0043] S2. Stir and mix Hypericum perforatum extract, Guaiac Wood extract, Mangosteen fruit peel extract, mango leaf extract, Polygonum multiflorum extract, Platycladus orientalis leaf extract, and ginger extract according to weight portions, and homogenize at 40°C at a homogenizing speed of 2000 rpm for 30 min. After the homogenization, stir continuously for 3 h, take out, and obtain a natural plant extract composition for preventing hair loss and strengthening hair.

[0044] Example 3

[0045] A natural plant extract composition for preventing hair loss and strengthening hair, comprising, by weight: 35 parts of Hypericum perforatum extract, 28 parts of Guaiacum jasminoides extract, 25 parts of Mangosteen fruit peel extract, 20 parts of mango leaf extract, 18 parts of Polygonum multiflorum extract, 11 parts of Platycladus orientalis leaf extract, and 9 parts of ginger extract.

[0046] The method for preparing the natural plant extract composition for preventing hair loss and strengthening hair comprises the following steps:

[0047] S1. Prepare Hypericum perforatum flower, guaiac wood, mangosteen peel, mango leaf, Polygonum multiflorum root, Platycladus orientalis leaf, and ginger, wash and dry, crush, and mix with anhydrous ethanol respectively, wherein the mass volume g / mL ratio of the Hypericum perforatum flower, guaiac wood, mangosteen peel, mango leaf, Polygonum multiflorum root, Platycladus orientalis leaf, ginger and anhydrous ethanol is 1:20, reflux extraction is performed at 110°C for 3 times, each time for 3 hours, and the three filtrates are combined after filtration, and the solvent is recovered by concentration under reduced pressure to obtain a concentrated solution, which is placed in an oven at 40°C for drying to obtain the corresponding Hypericum perforatum extract, guaiac wood extract, mangosteen peel extract, mango leaf extract, Polygonum multiflorum extract, Platycladus orientalis leaf extract, and ginger extract;

[0048] S2. Stir and mix Hypericum perforatum extract, Guaiac Wood extract, Mangosteen fruit peel extract, mango leaf extract, Polygonum multiflorum extract, Platycladus orientalis leaf extract, and ginger extract according to weight portions, and homogenize at 40°C at a homogenizing speed of 2000 rpm for 30 min. After the homogenization, stir continuously for 3 h, take out, and obtain a natural plant extract composition for preventing hair loss and strengthening hair.

[0049] Example 4

[0050] A natural plant extract composition for preventing hair loss and strengthening hair, comprising, by weight: 40 parts of Hypericum perforatum extract, 30 parts of Guaiacum jasminoides extract, 30 parts of Mangosteen fruit peel extract, 25 parts of mango leaf extract, 20 parts of Polygonum multiflorum extract, 12 parts of Platycladus orientalis leaf extract, and 10 parts of ginger extract.

[0051] The method for preparing the natural plant extract composition for preventing hair loss and strengthening hair comprises the following steps:

[0052] S1. Prepare Hypericum perforatum flower, guaiac wood, mangosteen peel, mango leaf, Polygonum multiflorum root, Platycladus orientalis leaf, and ginger, wash and dry, crush, and mix with anhydrous ethanol respectively, wherein the mass volume g / mL ratio of the Hypericum perforatum flower, guaiac wood, mangosteen peel, mango leaf, Polygonum multiflorum root, Platycladus orientalis leaf, ginger and anhydrous ethanol is 1:20, reflux extraction is performed at 110°C for 3 times, each time for 3 hours, and the three filtrates are combined after filtration, and the solvent is recovered by concentration under reduced pressure to obtain a concentrated solution, which is placed in an oven at 40°C for drying to obtain the corresponding Hypericum perforatum extract, guaiac wood extract, mangosteen peel extract, mango leaf extract, Polygonum multiflorum extract, Platycladus orientalis leaf extract, and ginger extract;

[0053] S2. Stir and mix Hypericum perforatum extract, Guaiac Wood extract, Mangosteen fruit peel extract, mango leaf extract, Polygonum multiflorum extract, Platycladus orientalis leaf extract, and ginger extract according to weight portions, and homogenize at 40°C at a homogenizing speed of 2000 rpm for 30 min. After the homogenization, stir continuously for 3 h, take out, and obtain a natural plant extract composition for preventing hair loss and strengthening hair.

[0054] Example 5

[0055] A natural plant extract composition for preventing hair loss and strengthening hair, comprising, by weight: 45 parts of Hypericum perforatum extract, 35 parts of Guaiacum jasminoides extract, 30 parts of Mangosteen fruit peel extract, 30 parts of mango leaf extract, 25 parts of Polygonum multiflorum extract, 15 parts of Platycladus orientalis leaf extract, and 12 parts of ginger extract.

[0056] The method for preparing the natural plant extract composition for preventing hair loss and strengthening hair comprises the following steps:

[0057] S1. Prepare Hypericum perforatum flower, guaiac wood, mangosteen peel, mango leaf, Polygonum multiflorum root, Platycladus orientalis leaf, and ginger, wash and dry, crush, and mix with anhydrous ethanol respectively, wherein the mass volume g / mL ratio of the Hypericum perforatum flower, guaiac wood, mangosteen peel, mango leaf, Polygonum multiflorum root, Platycladus orientalis leaf, ginger and anhydrous ethanol is 1:20, reflux extraction is performed 3 times at 120°C, each time for 3 hours, and the three filtrates are combined after filtration, and the solvent is recovered by concentration under reduced pressure to obtain a concentrated solution, which is placed in an oven at 40°C for drying to obtain the corresponding Hypericum perforatum extract, guaiac wood extract, mangosteen peel extract, mango leaf extract, Polygonum multiflorum extract, Platycladus orientalis leaf extract, and ginger extract;

[0058] S2. Stir and mix Hypericum perforatum extract, Guaiac Wood extract, Mangosteen fruit peel extract, mango leaf extract, Polygonum multiflorum extract, Platycladus orientalis leaf extract, and ginger extract according to weight portions, and homogenize at 40°C at a homogenizing speed of 2000 rpm for 30 min. After the homogenization, stir continuously for 3 h, take out, and obtain a natural plant extract composition for preventing hair loss and strengthening hair.

[0059] Comparative Example 1

[0060] The composition of this comparative example is different from that of Example 2 in that this comparative example does not contain the Hypericum perforatum extract, and the missing amount of the Hypericum perforatum extract is distributed to the corresponding components according to the ratio of the other six substances in Example 2.

[0061] Comparative Example 2

[0062] The composition of this comparative example is different from that of Example 2 in that this comparative example does not contain the guaiac extract, and the missing amount of the guaiac extract is distributed to the corresponding components according to the ratio of the other six substances in Example 2.

[0063] Comparative Example 3

[0064] The composition of this comparative example is different from that of Example 2 in that this comparative example does not contain the mangosteen peel extract, and the missing amount of the mangosteen peel extract is distributed to the corresponding components according to the ratios of the other six substances in Example 2.

[0065] Comparative Example 4

[0066] The composition of this comparative example is different from that of Example 2 in that this comparative example does not contain mango leaf extract, and the missing amount of mango leaf extract is distributed to the corresponding components according to the ratio of the other six substances in Example 2.

[0067] Comparative Example 5

[0068] The composition of this comparative example is different from that of Example 2 in that this comparative example does not contain the Polygonum multiflorum extract, and the missing amount of the Polygonum multiflorum extract is distributed to the corresponding components according to the ratio of the other six substances in Example 2.

[0069] Test Example 1 Antibacterial Test

[0070] The main microorganisms that cause seborrheic alopecia include aerobic fungi (Malassezia furfur), anaerobic bacteria (Propionibacterium acnes) and facultative aerobic bacteria (Staphylococcus aureus).

[0071] The test method is the Oxford cup method (tube and plate method), which is a common method for determining the potency of antibiotics at home and abroad, and is also a method specified in the pharmacopoeias of many countries. The inhibition zone test is used to determine the inhibitory effect of the sample on Malassezia furfur (Propionibacterium acnes ATCC 44344), Propionibacterium acnes (Propionibacterium acnesATCC11827), and Staphylococcus aureus (Staphylococcus aureus ATCC49775).

[0072] Test samples: The compositions prepared in Examples 1-5 and Comparative Examples 1-5 were diluted with dimethyl sulfoxide (DMSO) to a sample with a mass fraction of 5%, and the following test was performed.

[0073] Aseptic operation was performed and different culture methods were used for different microorganisms. Malassezia furfur was cultured with fungal culture medium and shaking, Propionibacterium acnes was cultured with anaerobic method, and Staphylococcus aureus was cultured with LB broth culture medium and shaking. The three activated bacteria were prepared into bacterial suspensions, and 0.2 mL of each bacterial suspension was smeared on Leeming & Notman culture medium plates. The Oxford cup was placed vertically on the surface of the corresponding culture medium with tweezers and gently pressed to make it in contact with the culture medium without gaps. Three small tubes could be placed on each plate, and 0.1 mL of various test samples were added to each small tube, and marked to prevent overflow. After anaerobic culture at 37°C incubator for 3 days, the results were observed.

[0074] During the culture, on the one hand, the test bacteria began to grow, and on the other hand, the sample to be tested spread outward from the Oxford cup as the origin, forming a circular area where the colony cannot grow, namely the "inhibition zone". The larger the inhibition zone, the better the acne removal effect. The experimental results were used to determine the antibacterial activity according to the diameter of the inhibition zone. The criteria for determining the antibacterial effect were: an inhibition zone diameter of >20mm is highly effective; an inhibition zone diameter of 15-20mm is moderately effective; an inhibition zone diameter of 10-15mm is inefficient; and an inhibition zone diameter of <10mm is ineffective. The antibacterial effect is shown in Table 1:

[0075] Table 1

[0076]

[0077]

[0078] As can be seen from Table 1, the composition of the embodiments of the present invention has a strong antibacterial effect on Malassezia furfur, Propionibacterium acnes, and Staphylococcus aureus, and the inhibition zone of the comparative composition on the bacteria is smaller than that of the embodiments, indicating that the composition of the present invention has a synergistic effect and can effectively inhibit Malassezia furfur, Propionibacterium acnes and Staphylococcus aureus that cause seborrheic alopecia.

[0079] Test Example 2 Effect of 5α-reductase activity

[0080] The main cause of seborrheic alopecia is that the 5α-reductase in the scalp hair follicles can convert testosterone into the more active dihydrotestosterone (DHT). The latter has an affinity for the androgen receptor (AR) more than 5 times greater than testosterone. DHT combines with AR concentrated in the hair follicle unit and enters the cell nucleus, thereby producing a series of biological effects, reducing the concentration of intracellular cyclic adenosine monophosphate, shortening the hair growth cycle, and miniaturizing the hair follicles, thereby causing hair loss.

[0081] The "one-step sandwich method" is used to detect the inhibition rate of the test substance on 5α-reductase. The inhibitory factor (may exist in the sample) acts on the 5α-reductase adsorbed on the antibody (the antibody has been fixed to the concave hole of the ELISA plate), inhibiting its activity. After the treatment, the 5α-reductase still remaining on the microwell is connected with the HRP-labeled immunoenzyme to catalyze the substrate to develop color. After terminating the reaction, the absorbance value is tested. By analyzing the data, the inhibitory effect of the test substance on 5α-reductase is evaluated, and the oil control effect of the sample is scientifically and reasonably evaluated based on this data. Test materials and instruments: ELISA analyzer; 5α-reductase antigen Elisa reagent test kit.

[0082] Test method:

[0083] ① Dilute the samples of Example 1-5 and Comparative Example 1-5 N times with 0.1mol / L Tris-HCl buffer as samples for the next test, take out the required slats from the aluminum bag after equilibration at room temperature for 20 minutes, seal the remaining slats with a ziplock bag and return them to 4°C. Note: The concentration of this sample should not be too high, as it will lead to an imbalance between the sample and the reductase concentration. A preliminary experiment is required for the most suitable concentration.

[0084] ② Set up blank wells, positive control wells, sample wells, and negative control wells; except for the blank group, no enzyme was added, and 50 μL of 5α-reductase was added to each well of the other groups, and incubated in a constant temperature box at 37°C for 10 min; add samples of Example 1-5 and Comparative Example 1-5 to the sample wells, and add positive control (EGGG with a certain molar concentration) to the positive control wells; note that the molar concentration ratio between the sample and the positive control should be appropriate (at least less than 10 times); no sample or inhibitor was added to the negative control; incubate the microplate after sample addition for 10 min, and wash the plate 5 times (the plate washing solution is provided by the kit, and the original washing solution is diluted 20 times for standby use).

[0085] ③ Add 100uL of horseradish peroxidase (HRP)-labeled detection antibody to each well of the negative control group, positive control group and sample wells, seal the reaction wells with a sealing film, and incubate at 37°C in a water bath or incubator for 60min.

[0086] ④Discard the liquid, pat dry on absorbent paper, and wash the plate 5 times.

[0087] ⑤ Add 50 μl of substrate A and B to each well and incubate at 37°C in the dark for 15 min.

[0088] ⑥ Add 50 μl of stop solution to each well and measure the OD value of each well at a wavelength of 450 nm within 15 minutes.

[0089] Calculation of 5α-reductase inhibition rate:

[0090] (1) The percentage calculation formula of sample inhibition rate is as follows:

[0091] Inhibition rate (%) = 100 × (OD 空白 +OD 阴性对照 -OD 样品 ) / (OD 空白 +OD 阴性对照 )

[0092] (2) The percentage calculation formula of the positive control inhibition rate is as follows:

[0093] Inhibition rate (%) = 100 × (OD 空白 +OD 阴性对照 -OD 阳性对照 ) / (OD 空白 +OD 阴性对照 )

[0094] Test results: The inhibition of the test samples on 5α-reductase is shown in Table 2.

[0095] Table 2

[0096] Group Inhibition rate (%) Example 1 83.6 Example 2 89.2 Example 3 91.3 Example 4 90.8 Example 5 88.5 Comparative Example 1 75.6 Comparative Example 2 69.1 Comparative Example 3 64.3 Comparative Example 4 73.2 Comparative Example 5 50.6

[0097] From the results in Table 2, it can be seen that the inhibition rate of 5α-reductase by the composition of the present invention is relatively high, while the inhibition rate of 5α-reductase by the comparative example composition is significantly lower than that in the embodiment, indicating that the synergistic enhancement of the composition of the present invention can better exert the effect of inhibiting the activity of 5α-reductase, thereby also having a better preventive and therapeutic effect on hair loss.

[0098] Application Examples 1-5

[0099] The natural plant extract composition for preventing hair loss and fixing hair of Examples 1-5 is mixed with auxiliary materials to prepare shampoo; the formula of the shampoo consists of the following components: 3% of the natural plant extract composition for preventing hair loss and fixing hair, 75% of distilled water, 5% of hexylene glycol, 5% of silicone oil, 9% of sodium laureth sulfate, 0.8% of cocamide, 0.7% of lauroyl hydroxysulfobetaine, 0.5% of phenoxyethanol and 1% of citric acid.

[0100] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A natural plant extract composition for preventing hair loss and strengthening hair, characterized in that: The invention comprises, by weight, 25-45 parts of Hypericum perforatum extract, 20-35 parts of Guaiac Wood extract, 15-30 parts of Mangosteen fruit peel extract, 10-30 parts of mango leaf extract, 10-25 parts of Polygonum multiflorum extract, 8-15 parts of Platycladus orientalis leaf extract and 6-12 parts of ginger extract.

2. A natural plant extract composition for preventing hair loss and strengthening hair according to claim 1, characterized in that: The invention comprises, by weight, 30-40 parts of Hypericum perforatum extract, 25-30 parts of Guaiac Wood extract, 20-30 parts of Mangosteen fruit peel extract, 15-25 parts of mango leaf extract, 15-20 parts of Polygonum multiflorum extract, 10-12 parts of Platycladus orientalis leaf extract and 8-10 parts of ginger extract.

3. A method for preparing a natural plant extract composition for preventing hair loss and strengthening hair according to any one of claims 1 to 2, characterized in that: The following steps are involved: S1. Prepare Hypericum perforatum flower, guaiac wood, mangosteen peel, mango leaf, Polygonum multiflorum root, Platycladus orientalis leaf, and ginger, wash, dry, crush, mix with alcohol solvents respectively, extract at 100-120° C., collect the extract, concentrate, and dry to obtain the corresponding Hypericum perforatum extract, guaiac wood extract, mangosteen peel extract, mango leaf extract, Polygonum multiflorum extract, Platycladus orientalis leaf extract, and ginger extract; S2. Stir and mix Hypericum perforatum extract, Guaiac Wood extract, Mangosteen fruit peel extract, mango leaf extract, Polygonum multiflorum extract, Platycladus orientalis leaf extract, and ginger extract according to weight portions, and homogenize at 30-40°C. After homogenization, stir continuously for 2-3 hours, take out, and obtain a natural plant extract composition for preventing hair loss and strengthening hair.

4. The method for preparing a natural plant extract composition for preventing hair loss and strengthening hair according to claim 3, characterized in that: The alcohol solvent includes anhydrous ethanol; the mass volume g / mL ratio of the Hypericum perforatum flower, guaiac wood, mangosteen peel, mango leaf, Polygonum multiflorum root, Platycladus orientalis leaf, ginger and anhydrous ethanol is 1:15-20.

5. Use of a natural plant extract composition for preventing hair loss and strengthening hair according to any one of claims 1 to 2 in the preparation of an additive for hair care products.

6. Use of a natural plant extract composition for preventing hair loss and strengthening hair according to any one of claims 1 to 2 in the preparation of hair care products.

7. A hair care product, comprising the natural plant extract composition for preventing hair loss and strengthening hair according to any one of claims 1 to 2, and auxiliary materials acceptable to hair care products.

8. The hair care product according to claim 7, characterized in that: The auxiliary materials include one or more of a solubilizing agent, a penetration enhancer, a surfactant, a thickening agent and a preservative.

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