Skin care composition containing pogostemon cablin and preparation method and cosmetic thereof

Abstract

The invention relates to the field of cosmetics, in particular to a skin care composition containing pogostemon cablin, a preparation method of the skin care composition and cosmetics. The invention provides a skin care composition containing pogostemon cablin, which is prepared from the following components in percentage by mass: 10-25% of tea tree oil, 10-25% of pogostemon cablin, 10-25% of pogostemon cablin, 10-25% of pogostemon cablin, 10-25% of 15-30% of pogostemon cablin volatile oil; and 50 to 65% of evening primrose oil. According to the composition disclosed by the invention, all the components generate a synergistic effect, so that the inhibition effect on a propionibacterium acnes S21016 strain and staphylococcus aureus is improved; the anti-inflammatory effect is realized. The propionibacterium acnes and the staphylococcus aureus are closely related to the acnes; by inhibiting growth and reproduction of propionibacterium acnes and staphylococcus aureus bacteria, the effect of treating the acnes through bacteriostasis and sterilization is achieved.

Description

Technical Field

[0001] This application relates to the field of cosmetics, and more specifically, to a skin care composition containing patchouli, a method for preparing the same, and cosmetics. Background Technology

[0002] Acne causes numerous skin damages, including inflammatory damage: red, swollen pimples (papules / pustules) lead to dilation of capillaries in the dermis, causing localized redness, swelling, and pain. Deep inflammation can even lead to tissue necrosis, forming hard lumps. Furthermore, long-term acne can damage the skin barrier. The skin around pimples experiences increased transepidermal water loss, resulting in dryness and flaking. It becomes more susceptible to external stimuli (bacteria, pollutants).

[0003] However, common acne treatment products on the market have problems such as poor acne treatment effect, complex ingredients, or strong irritation; therefore, there is a need to develop a new acne treatment product. Summary of the Invention

[0004] The purpose of this application is to provide a skin care composition containing patchouli, its preparation method, and a cosmetic.

[0005] In a first aspect, this application provides a skincare composition containing patchouli, wherein the skincare composition containing patchouli comprises the following ingredients in percentage by weight:

[0006] Tea tree oil: 10-25%;

[0007] Patchouli volatile oil: 15-30%; and

[0008] Evening primrose oil: 50-65%.

[0009] In the various compositions of this application, the components exhibit a synergistic effect, enhancing the inhibitory effect against Propionibacterium acnes S21016 and Staphylococcus aureus; and also possessing anti-inflammatory properties. Both Propionibacterium acnes and Staphylococcus aureus are closely related to acne formation; by inhibiting the growth and reproduction of these bacteria, it is beneficial to achieve the efficacy of treating acne through antibacterial and bactericidal action.

[0010] In other embodiments of this application, the skin care composition containing patchouli comprises, by weight percentage, the following ingredients:

[0011] Tea tree oil: 15-25%;

[0012] Patchouli volatile oil: 20-30%; and

[0013] Evening primrose oil: 55-65%.

[0014] In other embodiments of this application, the skin care composition containing patchouli comprises, by weight percentage, the following ingredients:

[0015] Tea tree oil: 20-25%;

[0016] Patchouli volatile oil: 25-30%; and

[0017] Evening primrose oil: 55-60%.

[0018] In other embodiments of this application, the preparation steps of patchouli volatile oil include:

[0019] Patchouli branches and leaves were pulverized to obtain patchouli branch and leaf powder;

[0020] The powdered branches and leaves of patchouli were extracted by steam distillation to obtain a volatile water-oil mixture;

[0021] The volatile water-oil mixture is dried.

[0022] In other embodiments of this application, the volatile water-oil mixture is dried with anhydrous magnesium sulfate.

[0023] In other embodiments of this application, the mass ratio of the tea tree oil, the patchouli volatile oil, and the evening primrose oil is (10-25):(15-30):(50-65).

[0024] In other embodiments of this application, the mass ratio of the tea tree oil, the patchouli volatile oil, and the evening primrose oil is (15-25):(20-30):(55-65).

[0025] Secondly, this application provides a method for preparing a skin care composition containing patchouli, comprising:

[0026] Patchouli branches and leaves were pulverized to obtain patchouli branch and leaf powder;

[0027] The powdered branches and leaves of patchouli were extracted by steam distillation to obtain a volatile water-oil mixture;

[0028] A volatile water-oil mixture was dried with anhydrous magnesium sulfate to obtain patchouli volatile oil;

[0029] Mix the patchouli volatile oil, tea tree oil, and evening primrose oil evenly.

[0030] Thirdly, this application provides a cosmetic product comprising the patchouli-containing skincare composition of any of the foregoing claims.

[0031] In other embodiments of this application, the amount of patchouli-containing skincare composition added to the cosmetic is 1 to 10% by weight. Detailed Implementation

[0032] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions in the embodiments of this application will be clearly and completely described below. Obviously, the described embodiments are some embodiments of this application, but not all embodiments.

[0033] Therefore, the following detailed description of the embodiments of this application is not intended to limit the scope of the claimed application, but merely to illustrate selected embodiments of the application. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

[0034] This application provides a skin care composition containing patchouli, wherein the skin care composition containing patchouli comprises the following components in percentage by weight:

[0035] Tea tree oil: 10-25%;

[0036] Patchouli volatile oil: 15-30%; and

[0037] Evening primrose oil: 50-65%.

[0038] Exemplary examples, in some embodiments of this application, the skin care composition containing patchouli comprises, by weight percentage, the following ingredients:

[0039] Tea tree oil: 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, or any range between two of the aforementioned values;

[0040] Patchouli volatile oil: 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, or any range between two of the aforementioned values; and

[0041] Evening primrose oil: 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, or any range between any two of the aforementioned values.

[0042] In the various compositions of this application, the components exhibit a synergistic effect, enhancing the inhibitory effect against Propionibacterium acnes S21016 and Staphylococcus aureus; and also possessing anti-inflammatory properties. Both Propionibacterium acnes and Staphylococcus aureus are closely related to acne formation; by inhibiting the growth and reproduction of these bacteria, it is beneficial to achieve the efficacy of treating acne through antibacterial and bactericidal action.

[0043] In the above technical solution, tea tree oil possesses strong antibacterial capabilities. Its main component, terpine-4-ol, can disrupt the cell membrane structure of bacteria, causing intracellular substances to leak out, thereby inhibiting the growth and reproduction of bacteria closely related to acne formation, such as Propionibacterium acnes and Staphylococcus aureus. Patchouli volatile oil has good antibacterial effects, inhibiting the growth and reproduction of acne-related bacteria such as Propionibacterium acnes, thus reducing inflammation caused by bacterial infection and helping to alleviate acne symptoms. Additionally, it can regulate sebum secretion, maintaining a relatively balanced state and reducing the likelihood of acne caused by sebum clogging hair follicles. Evening primrose oil is rich in polyunsaturated fatty acids such as gamma-linolenic acid (GLA). GLA can regulate the human endocrine system, especially improving acne problems caused by endocrine disorders. It can also reduce skin inflammation and greatly alleviate the pain of acne. The above technical solution, by combining the three ingredients within the aforementioned proportions, greatly enhances the antibacterial and anti-inflammatory effects of the composition.

[0044] In the above technical solution, the MIC values ​​of tea tree oil against Propionibacterium acnes S21016 and Staphylococcus aureus were as follows: the MIC value of tea tree oil against Propionibacterium acnes S21016 was 0.20 μL / mL, and the MIC value against Staphylococcus aureus was 0.15 μL / mL. Combining it with patchouli oil and evening primrose oil within the above range can further enhance the antibacterial effect of the entire composition.

[0045] Further optionally, in some embodiments of this application, the skin care composition containing patchouli consists of the following components in percentage by weight: tea tree oil: 15-25%; patchouli volatile oil: 20-30%; and evening primrose oil: 55-65%.

[0046] Further optionally, in some embodiments of this application, the skin care composition containing patchouli consists of the following components in percentage by weight: tea tree oil: 20-25%; patchouli volatile oil: 25-30%; and evening primrose oil: 55-60%.

[0047] Furthermore, in some embodiments of this application, the preparation steps of patchouli volatile oil include:

[0048] Patchouli branches and leaves were pulverized to obtain patchouli branch and leaf powder;

[0049] The powdered branches and leaves of patchouli were extracted by steam distillation to obtain a volatile water-oil mixture;

[0050] The volatile water-oil mixture is dried.

[0051] Furthermore, in some embodiments of this application, the volatile water-oil mixture is dried using anhydrous magnesium sulfate.

[0052] The above technical solution, through "drying the volatile water-oil mixture," aims to reduce the impurity water content in the oil, improve purity, and thus reduce quality risks such as rancidity. It also avoids the risk of microbial contamination. This, in turn, helps to improve the antibacterial and anti-inflammatory effects of the entire composition.

[0053] Furthermore, in some embodiments of this application, the mass ratio of the tea tree oil, the patchouli volatile oil, and the evening primrose oil is (10-25):(15-30):(50-65).

[0054] The mass ratio of the tea tree oil, the patchouli volatile oil, and the evening primrose oil is (15-25):(20-30):(55-65).

[0055] This application provides a method for preparing a skin care composition containing patchouli, comprising:

[0056] Patchouli branches and leaves were pulverized to obtain patchouli branch and leaf powder;

[0057] The powdered branches and leaves of patchouli were extracted by steam distillation to obtain a volatile water-oil mixture;

[0058] A volatile water-oil mixture was dried with anhydrous magnesium sulfate to obtain patchouli volatile oil;

[0059] Mix the patchouli volatile oil, tea tree oil, and evening primrose oil evenly.

[0060] Some embodiments of this application provide a cosmetic product comprising the patchouli-containing skincare composition provided in any of the foregoing embodiments.

[0061] Furthermore, in some embodiments of this application, the amount of patchouli-containing skincare composition added to the cosmetic is 1 to 10% by mass percentage.

[0062] For example, in some embodiments of this application, the amount of patchouli-containing skincare composition added to the cosmetic, by weight percentage, is 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, or any two of the aforementioned values.

[0063] In some embodiments of this application, the cosmetics described above also include cosmetic carriers commonly used in the art, such as emulsifiers and thickeners.

[0064] The features and performance of this application will be further described in detail below with reference to embodiments:

[0065] The tea tree oil used in the examples or comparative examples was purchased from: Wilfen (Beijing) Technology Development Co., Ltd.

[0066] Evening primrose oil purchased from: Wilfen (Beijing) Technology Development Co., Ltd.

[0067] Examples and Comparative Examples

[0068] A skincare composition is provided, and the composition and content of each embodiment and comparative example are shown in Table 1:

[0069] Table 1 (wt%)

[0070] Tea tree oil Patchouli volatile oil Evening Primrose Oil Example 1 15 20 65 Example 2 20 25 55 Example 3 25 30 55 Example 4 15 20 50 Example 5 15 15 65 Example 6 10 20 65 Comparative Example 1 / / 65 Comparative Example 2 15 / / Comparative Example 3 / 20 / Comparative Example 4 25 30 / Comparative Example 5 / 30 55 Comparative Example 6 25 / 55

[0071] The preparation steps of the patchouli volatile oil in the above embodiments or comparative examples are as follows:

[0072] Patchouli branches and leaves were pulverized to obtain patchouli branch and leaf powder;

[0073] The powdered branches and leaves of patchouli were extracted by steam distillation to obtain a volatile water-oil mixture;

[0074] A volatile water-oil mixture was dried with anhydrous magnesium sulfate to obtain patchouli volatile oil.

[0075] The preparation steps of the above composition include:

[0076] Accurately weigh the ingredients according to the composition of each ingredient in Table 1, mix them thoroughly, and then dispense and seal them in dropper essential oil bottles.

[0077] The performance of the composition samples prepared in each embodiment and comparative example was tested.

[0078] 1. Determination of the MIC values ​​of the compositions of each embodiment or comparative example against Propionibacterium acnes S21016 strain and Staphylococcus aureus.

[0079] The testing method is as follows:

[0080] Step (1) Preparation of bacterial suspension

[0081] The strains were inoculated onto solid culture medium for activation (Propionibacterium acnes was cultured anaerobically for 48 hours, Staphylococcus aureus was cultured aerobically for 24 hours);

[0082] Pick a single colony and add it to sterile physiological saline, shake to mix, and adjust the turbidity to 0.5 McFarland standard (≈1.5×10 CFU / mL);

[0083] Dilute the bacterial culture with culture medium to 1×10 CFU / mL (working concentration).

[0084] Step (2): Establish the concentration gradient, see Table 2:

[0085] Table 2

[0086]

[0087] Perform the dilution operation according to the table above:

[0088] Test tube 1: Add 1.6 mL of culture medium and 0.4 mL of each example or comparative sample to dilute to a concentration of 10 mg / mL;

[0089] Test tube 2: Take 1 mL of sample and 1 mL of culture medium from test tube 1 and dilute to a concentration of 5 mg / mL;

[0090] Repeat the double dilution to 10 test tubes (final volume 1 mL / tube).

[0091] Step (3), Adding bacteria and culturing

[0092] Add 100 μL of working bacterial solution (final bacterial count ≈ 5 × 10 CFU / mL) to each test tube.

[0093] Control group setup:

[0094] Growth control: Culture medium + bacterial suspension (no sample)

[0095] Solvent control: Culture medium + bacterial suspension + 1% DMSO (to verify that the solvent has no antibacterial properties)

[0096] Blank control: Culture medium (sterile, to verify sterility).

[0097] Cultivation conditions:

[0098] Propionibacterium acnes: Incubate at 37℃ in an anaerobic jar (85% N, 10% H, 5% CO) for 48 hours;

[0099] Staphylococcus aureus: Aerobic culture at 37℃ for 24 hours;

[0100] Step (4) Result Interpretation

[0101] Visual observation: The minimum sample concentration that completely inhibits the growth of visible bacteria is defined as the MIC.

[0102] Verification method:

[0103] Take the contents of the MIC concentration test tube, spread it on an agar plate, and incubate to confirm that no viable bacteria are growing (to avoid misjudgment due to the quiescent effect).

[0104] The micro-method can be performed by staining with resazurin: a pink color indicates the presence of viable bacteria (MIC is more sensitive for interpretation).

[0105] The test results for each embodiment or comparative sample are shown in Table 3:

[0106] Table 3

[0107]

[0108]

[0109] The experimental results above show that:

[0110] The samples from each embodiment showed superior MIC values ​​against both Propionibacterium acnes S21016 and Staphylococcus aureus compared to the comparative examples. This demonstrates that the components in the various compositions of this application exhibit a synergistic effect, enhancing the inhibitory effect against Propionibacterium acnes S21016 and Staphylococcus aureus.

[0111] Propionibacterium acnes and Staphylococcus aureus are both closely related to acne formation; inhibiting the growth and reproduction of Propionibacterium acnes and Staphylococcus aureus can help achieve the effect of treating acne through antibacterial and bactericidal treatment.

[0112] Furthermore, compared with Example 4, it can be seen that, under the premise that the contents of tea tree oil and patchouli volatile oil are the same, reducing the content of evening primrose oil slightly reduces the inhibitory effect of the composition of Example 4 on Propionibacterium acnes and Staphylococcus aureus.

[0113] Furthermore, compared with Example 5, it can be seen that, under the premise of the same content of tea tree oil and evening primrose oil, reducing the content of patchouli volatile oil slightly reduces the inhibitory effect of the composition of Example 5 on Propionibacterium acnes and Staphylococcus aureus.

[0114] Furthermore, compared with Example 6, it can be seen that, under the premise that the contents of patchouli volatile oil and evening primrose oil are the same, reducing the content of tea tree oil slightly reduces the inhibitory effect of the composition of Example 6 on Propionibacterium acnes and Staphylococcus aureus.

[0115] Based on Tables 1 and 3, further optimization can be made. When the ratio of tea tree oil, patchouli volatile oil, and evening primrose oil is (15-25):(20-30):(55-65), it is more conducive to improving the inhibitory effect of the entire composition on Propionibacterium acnes and Staphylococcus aureus.

[0116] 2. Test samples of the compositions of each embodiment or comparative example in an inflammation model of THP-1 cells stimulated by Propionibacterium acnes (C. acnes).

[0117] The testing steps are as follows:

[0118] (1) THP-1 cell culture

[0119] Culture medium: RPMI 1640 + 10% FBS + 1% Pen / Strep

[0120] Culture conditions: 37℃, 5% CO2, density maintained at 2-5 × 10 cells / mL (>95% viability)

[0121] Subculturing: Centrifuge at 1500 rpm for 5 minutes, then dilute and subculture at a ratio of 1:3 (every 2-3 days).

[0122] (2) PMA-induced differentiation of macrophages

[0123] The operation is as follows:

[0124] Plating: Adjust THP-1 cells to 1×10 cells / mL and seed in 96-well plates (200μL / well).

[0125] Add PMA: Final concentration 100 nM PMA (1 mM stock solution prepared with DMSO)

[0126] Differentiation: After 48 hours of culture, cells adhere and extend (macrophage morphology).

[0127] Medium change: Discard the PMA-containing medium, wash twice with pre-warmed PBS, replace with fresh PMA-free medium, and let stand for 24 hours.

[0128] (3) Preparation of Propionibacterium acnes (C. acnes)

[0129] Strain activation: Anaerobic chamber (37℃, 80% N + 10% H + 10% CO), cultured in RCM medium for 5 days;

[0130] Bacterial collection: Centrifuge at 5000 rpm for 10 min, wash 3 times with PBS;

[0131] Heat inactivation: 80℃ water bath for 30 minutes (inactivation verification: no sterile growth after 7 days of plate incubation);

[0132] Concentration adjustment: Resuspend in PBS and adjust to 1×10 CFU / mL (equivalent to OD=0.5) using McFarland turbidimetry.

[0133] (4) Establishment of inflammation model and sample processing

[0134] Grouped design (96-well plate, n=6 replicates):

[0135] Blank control group: cells + culture medium

[0136] Negative control group: cells + PBS (solvent control)

[0137] Inflammation model group: cells + inactivated C. acnes (MOI = 10:1)

[0138] Low / medium / high dose sample groups: Cells + C. acnes + sample (pretreated for 1 hour)

[0139] Positive control group: Cells + C. acnes + dexamethasone (10 μM)

[0140] Stimulus conditions:

[0141] C. acnes was added at an MOI of 10:1 (bacteria:cells).

[0142] Add bacteria 1 hour after sample pretreatment

[0143] Co-cultured for 24 hours (37℃, 5% CO2).

[0144] (5) Detection of inflammatory factors

[0145] Sample collection: Centrifuge (3000 rpm, 10 min) and collect the supernatant. Detect IL-1β using ELISA (R&D Systems kit).

[0146] Cell lysates were detected by qRT-PCR; 2 -ΔΔCt The relative expression level of TLR2 mRNA was calculated using this method.

[0147] The test results are shown in Table 4 below.

[0148] Table 4

[0149]

[0150] The experimental results above show that:

[0151] The IL-1β and TLR2 mRNA levels in the samples of each embodiment were higher than those in the comparative examples. This indicates that the components in the various compositions of this application have a synergistic effect, enhancing the anti-inflammatory effect. This also demonstrates that the compositions of this application can inhibit the growth and reproduction of acne-related bacteria such as Propionibacterium acnes, thereby reducing inflammation caused by bacterial infection and helping to alleviate acne symptoms.

[0152] The secretion inhibition rate of the inflammatory cytokine IL-1β was 66.54%, and it significantly reduced the transcription of intracellular Toll-like receptor 2 (TLR2) mRNA, with an inhibition rate of 73.73%.

[0153] It is evident that the composition provided in the embodiments of this application is beneficial for achieving the effect of treating acne through antibacterial and bactericidal action.

[0154] Furthermore, compared with Example 4, it can be seen that, under the premise that the contents of tea tree oil and patchouli volatile oil are the same, reducing the content of evening primrose oil slightly reduces the inhibitory effect of the composition of Example 4 on Propionibacterium acnes and Staphylococcus aureus.

[0155] Furthermore, compared with Example 5, it can be seen that, under the premise of the same content of tea tree oil and evening primrose oil, reducing the content of patchouli volatile oil slightly reduces the inhibitory effect of the composition of Example 5 on Propionibacterium acnes and Staphylococcus aureus.

[0156] Furthermore, compared with Example 6, it can be seen that, under the premise that the contents of patchouli volatile oil and evening primrose oil are the same, reducing the content of tea tree oil slightly reduces the inhibitory effect of the composition of Example 6 on Propionibacterium acnes and Staphylococcus aureus.

[0157] Based on Tables 1 and 4, further optimization can be made. When the ratio of tea tree oil, patchouli volatile oil, and evening primrose oil is (15-25):(20-30):(55-65), it is more conducive to improving the inhibitory effect of the entire composition on Propionibacterium acnes and Staphylococcus aureus.

[0158] 3. Perform in vitro inflammatory cell experiments on the test samples of the compositions of each embodiment or comparative example.

[0159] Cellular experiments: In the human primary arthritis chondrocyte experiment, an in vitro inflammatory cell model was constructed. The cells were divided into a normal control group, an inflammation model group (chondrocytes were treated with 10 ng / mL interleukin-1β (IL-1β) for 24 h), and a group of GLA (10 μM) + IL-1β of various examples or comparative sample compositions (chondrocytes were treated with different composition samples for 2 h, and then treated with 10 ng / mL IL-1β for 24 h).

[0160] The test results of each embodiment or comparative example are shown in Table 5 below.

[0161] Table 5

[0162]

[0163]

[0164] The experimental results above show that:

[0165] The samples in each embodiment showed superior levels of TNF-α, IL-6, and IL-8 compared to the comparative examples. This indicates that the components in the various compositions of this application exhibit a synergistic effect, enhancing the anti-inflammatory efficacy. This also demonstrates that the compositions of this application can inhibit the growth and reproduction of acne-related bacteria such as Propionibacterium acnes, thereby reducing inflammation caused by bacterial infection and helping to alleviate acne symptoms.

[0166] The results showed that the samples from each embodiment could inhibit the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in chondrocytes induced by IL-1β, reduce the release of nitric oxide (NO) and prostate E2 (PGE2), and inhibit the expression of inflammatory cytokines TNF-α, IL-6 and IL-8 in chondrocytes induced by IL-1β. They also inhibited the expression of p-p65 and the nuclear translocation of p65 in chondrocytes. All the differences were statistically significant (P < 0.05).

[0167] Combining Tables 1 and 5, further optimization can be made. When the ratio of tea tree oil, patchouli volatile oil and evening primrose oil is (15-25):(20-30):(55-65), it is more conducive to improving the anti-inflammatory effect of the entire composition.

[0168] 4. Clinical testing protocols for samples from each embodiment or comparative example:

[0169] Thirty participants aged 18-30 with acne were recruited and randomly divided into three groups of 10 each. They were given a trial of the skincare compositions provided in Examples 1-3 above. The method of application was to apply the skincare composition to the acne-affected areas twice daily, morning and evening, for 7 consecutive days. A combination of professional ratings and subjective ratings from the participants was used. The rating results after 7 days of use are shown in Tables 6-8 below:

[0170] Table 6. Scoring results of the skincare composition in Example 1.

[0171]

[0172]

[0173] Table 7. Scoring results of the skincare composition in Example 2

[0174]

[0175] Table 8. Scoring results of the skincare composition in Example 3.

[0176]

[0177]

[0178] Explanation of Tables 6-8:

[0179] Acne size rating: No change is 0 points, slight reduction is 2 points, reduction is 4 points; significant reduction is 6 points, very significant reduction is 8 points, disappearance is 10 points;

[0180] Redness and swelling severity rating: No change is 0 points, slight improvement is 2 points, some change is 4 points; significant improvement is 6 points, very significant improvement is 8 points, disappearance is 10 points;

[0181] Pain rating: No change is 0 points, slight improvement is 2 points, some change is 4 points; significant improvement is 6 points, very significant improvement is 8 points, and no pain is 10 points;

[0182] The scoring results in Tables 6-8 above show that:

[0183] In Examples 1-3, the pimple size score was around 6 points, indicating that the composition of this application can significantly reduce pimple size after 7 days of use; in Examples 1-3, the redness and swelling score was around 6 points, indicating that the redness and swelling can significantly decrease after 7 days of use; in Examples 1-3, the pain score was around 8 points, indicating that the pain can be significantly reduced after 7 days of use.

[0184] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A skin care composition containing patchouli, characterized in that, The patchouli-containing skincare composition comprises, by weight percentage, the following ingredients: Tea tree oil: 10-25%; Patchouli volatile oil: 15-30%; and Evening primrose oil: 50-65%.

2. The skin care composition containing patchouli according to claim 1, characterized in that, The patchouli-containing skincare composition comprises, by weight percentage, the following ingredients in the following proportions. composition: Tea tree oil: 15-25%; Patchouli volatile oil: 20-30%; and Evening primrose oil: 55-65%.

3. The skin care composition containing patchouli according to claim 1, characterized in that, The patchouli-containing skincare composition comprises, by weight percentage, the following ingredients: Tea tree oil: 20-25%; Patchouli volatile oil: 25-30%; and Evening primrose oil: 55-60%.

4. The skin care composition containing patchouli according to any one of claims 1-3, characterized in that, The preparation steps of the patchouli volatile oil include: Patchouli branches and leaves were pulverized to obtain patchouli branch and leaf powder; The patchouli leaf powder was extracted by steam distillation to obtain a volatile water-oil mixture; The volatile water-oil mixture is dried.

5. The skin care composition containing patchouli according to claim 4, characterized in that, Volatile water-oil mixtures are dried using anhydrous magnesium sulfate.

6. The skin care composition containing patchouli according to claim 5, characterized in that, The mass ratio of the tea tree oil, the patchouli volatile oil, and the evening primrose oil is (10-25):(15-30):(50-65).

7. The skin care composition containing patchouli according to claim 6, characterized in that, The mass ratio of the tea tree oil, the patchouli volatile oil, and the evening primrose oil is (15-25):(20-30):(55-65).

8. The method for preparing the skin care composition containing patchouli according to any one of claims 1-7, characterized in that, include: Patchouli branches and leaves were pulverized to obtain patchouli branch and leaf powder; The powdered branches and leaves of patchouli were extracted by steam distillation to obtain a volatile water-oil mixture; A volatile water-oil mixture was dried with anhydrous magnesium sulfate to obtain patchouli volatile oil; Mix the patchouli volatile oil, tea tree oil, and evening primrose oil evenly.

9. A cosmetic product, characterized in that, The cosmetic product includes the skin care composition containing patchouli as described in any one of claims 1-8.

10. The cosmetic product according to claim 9, characterized in that, The amount of the patchouli-containing skincare composition added to the cosmetic product is 1-10% by weight.

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