Antibacterial peptide Scybaumancin105-127 and application thereof

A technology of antibacterial peptides and antibacterial agents, applied in the field of peptides and their applications, can solve problems such as no drug availability, and achieve the effects of safe and non-toxic antibacterial activity, strong antibacterial activity, and high antibacterial activity.

Active Publication Date: 2021-07-16
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the continuous use of antibiotics, the inappropriate or excessive use of antibiotics has continuously enriched and spread the bacterial resistance genes, resulting in the emergence of a large number of drug-resistant bacteria in the clinic, which has brought great harm to clinical treatment. challenges, and even the situation where no medicine is available

Method used

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  • Antibacterial peptide Scybaumancin105-127 and application thereof
  • Antibacterial peptide Scybaumancin105-127 and application thereof
  • Antibacterial peptide Scybaumancin105-127 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Antimicrobial peptide Scybaumancin 105-127

[0036] A kind of antimicrobial peptide Scybaumancin of the present embodiment 105-127 The amino acid sequence is shown in SEQ ID NO.1, specifically:

[0037] V-V-T-C-R-L-A-H-M-A-L-K-S-A-K-S-S-R-S-L-L-C-H-NH 2 , C-terminal amidation modification (-NH 2 ).

[0038] In this example, Nanjing GenScript Co., Ltd. was entrusted to synthesize the antimicrobial peptide Scybaumancin with a purity of more than 95% by solid phase synthesis. 105-127 , and provide detection information such as polypeptide molecular weight and HPLC, use Helixquest to predict its charge and hydrophobicity, and use ProtParam to predict other physical and chemical parameters, and the antimicrobial peptide Scybaumancin 105-127 The physical and chemical parameters are shown in Table 1.

[0039] Table 1 Antimicrobial peptide Scybaumancin 105-127 Physical and chemical parameters

[0040]

Embodiment 2

[0041] Example 2: Scybaumancin 105-127 Determination of minimum bactericidal concentration (MBC: Minimum Bactericidal Concentration)

[0042] 1. The bacterial strains involved in this embodiment include: Listeria monocytogenes (Listeriamonocytogenes), Enterococcus faecium (Enterococcus faecium), Shigella flexneri (Shigellaflexneri), Acinetobacter baumannii (Acinetobacter baumannii) , Escherichia coli (Escherichia coli), Pseudomonas aeruginosa (Pseudomonas aeruginosa), and Cryptococcus neoformans (Cryptococcus neoformans) were purchased from the Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences; Propionibacterium acnes (Propionibacterium acnes) were purchased from Guangdong Provincial Microbial Culture Collection Center; clinical drug-resistant bacteria QZ18080, QZ18081, QZ18050, QZ18055, QZ18109, QZ18110 were from the Laboratory Department of the Second Affiliated Hospital of Fujian Medical University.

[0043] 2. The specific method is a...

Embodiment 3

[0057] Example 3: Scybaumancin 105-127 Kinetic curve of sterilization

[0058] 1. The strains involved are: Acinetobacter baumannii CGMCC 1.6769 and Cryptococcus neoformans CGMCC 2.1563.

[0059] 2. The specific method is as follows:

[0060] The antibacterial test method is consistent with the determination of the minimum bactericidal concentration. After co-incubating the antimicrobial peptide and bacteria for a certain period of time, take 10 μL of the co-incubated mixture to 490 μL PBS at different time points, mix well, and take 50 μL of the diluted solution to smear the NB plate. If the pre-experiment If the number of colonies on the coated plate is less than 50 clones, take 25 μL of the co-incubated mixture and smear it directly on the plate. After standing in a 37°C incubator for 12 hours, count the colonies (the mixed solution of the fungal co-incubation is diluted 10 times with PBS, then coated with YPD plates were placed in an incubator at 28°C for 48 hours, and c...

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Abstract

The invention discloses an antibacterial peptide Scybaumancin105-127 and application thereof, the antibacterial peptide is found in Scylla paramamosain for the first time, the molecular formula of the antibacterial peptide is C106H188N36O28S3, and the amino acid sequence of the antibacterial peptide is as shown in SEQ ID NO.1. The antibacterial peptide Scybaumancin105-127 disclosed by the invention has good antibacterial activity on acinetobacter baumannii, shigella flexneri, escherichia coli, pseudomonas aeruginosa, cryptococcus neoformans, listeria monocytogenes, enterococcus faecium and propionibacterium acnes, and has no cytotoxicity on normal cells. The antibacterial peptide Scybaumancin105-127 has high antibacterial activity, safety and non-toxic, also has relatively strong antibacterial activity on clinical drug-resistant bacteria, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of marine molecular biology, and specifically relates to polypeptides and applications thereof. Background technique [0002] Since Fleming discovered penicillin in 1928, the popularization and application of antibiotics has saved countless lives and promoted the development of modern medicine. However, during the continuous use of antibiotics, the inappropriate or excessive use of antibiotics has continuously enriched and spread the bacterial resistance genes, resulting in the emergence of a large number of drug-resistant bacteria in the clinic, which has brought great harm to clinical treatment. challenges, and even the situation where no medicine is available. The problem of bacterial resistance has spread globally. In 2017, the World Health Organization published the first list of "key pathogens" for antibiotic resistance, and released a catalog of 12 bacterial species that pose a great threat to human ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435A61K38/17A61P31/04A61P31/10A23L3/3535A01N47/44A01P1/00A01P3/00
CPCC07K14/43509A61P31/04A61P31/10A23L3/3535A01N47/44A61K38/00A23V2002/00A23V2200/10A23V2250/55Y02A50/30
Inventor 王克坚周瑛陈芳奕彭会陈慧芸
Owner XIAMEN UNIV
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