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Bionic artificial bone scaffold and preparation method thereof

A kind of artificial bone and biological technology, which is applied in the field of bionic artificial bone scaffold and its preparation, can solve the problems of slow crawling replacement, difficulty in realizing the complete repair and reconstruction of the defect area of ​​scaffold and osseointegration, and achieve enhanced ability to repair bone defects and facilitate Nutrient delivery and cell metabolic waste discharge, the effect of a wide range of sources

Active Publication Date: 2017-03-15
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the common problem with the existing artificial bone scaffolds is that although the interface between the implant and the bone can form a certain osseointegration, the new bone growth is limited by the local microenvironment, and the crawling replacement is slow. , Osteogenesis is limited to the edge area of ​​the implant, it is difficult to achieve the integration of the scaffold and the bone and the complete repair and reconstruction of the defect area
(Wu S, et al.Fabrication of aligned nanofiberpolymer yarn networks for anisotropic soft tissuescaffolds.ACS Appl.Mater.Interfaces 2016(8):16950-60) So far, no electrospun nanofibers have been constructed in a layered, interlaced helical manner. Literature reports on bionic artificial bone scaffolds for bone defect repair

Method used

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  • Bionic artificial bone scaffold and preparation method thereof
  • Bionic artificial bone scaffold and preparation method thereof
  • Bionic artificial bone scaffold and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Dissolve type I collagen and biodegradable polymers in a composite solvent of hexafluoroisopropanol and dimethylformamide at a mass ratio of 99:1 (volume ratio of hexafluoroisopropanol to dimethylformamide 2:1), and add cell growth factors to prepare material solution A, wherein the degradable polymer is polylactide-glycolide; cell growth factors are VEGF and b-FGF, in material solution A The mass fractions are 0.001% and 0.00001%, respectively;

[0041] (2) adding nano-hydroxyapatite (n-HA) to the type I collagen / polylactide-glycolide mixed solution prepared in step (1), and adding cell growth factors to prepare material solution B, Among them, n-HA is composed of Ca(OH) 2 and phosphoric acid according to the molar ratio of calcium / phosphorus of 1.67:1 under the condition that pH is 10, and the mass fraction in material solution B is 20%; the cell growth factors are BMP-7 and OGP, in material solution B The mass fractions are 0.005% and 0.00002%, respectively;

...

Embodiment 2

[0047] (1) Type I collagen and biodegradable polymer are dissolved in acetic acid solution with a mass fraction of 3% according to the mass ratio of 80:20, and cell growth factors are added to obtain material solution A, wherein the degradable polymer is The mixture of chitosan and hyaluronic acid (the mass ratio of chitosan and hyaluronic acid is 7:3); cell growth factor is VEGF, Ang-1 and Ang-2, and the mass fraction in material solution A is respectively 0.001%, 0.0001% and 0.0001%;

[0048] (2) adding nano-hydroxyapatite (n-HA) to the mixed solution of type I collagen / chitosan / hyaluronic acid prepared in step (1), and adding cell growth factors to obtain material solution B, Among them, n-HA is composed of Ca(NO 3 ) 2 and (NH 4 ) 2 HPO 4 According to the calcium / phosphorus molar ratio 1.67:1, the reaction synthesis under the condition of pH 11, the mass fraction in the material solution B is 60%; the cell growth factors are BMP-2 and TGF-β, in the material solution B ...

Embodiment 3

[0054](1) Dissolve type I collagen and biodegradable polymers in dimethyl sulfoxide at a mass ratio of 70:30, and add cell growth factors to prepare material solution A, wherein the degradable polymers are polylactic acid and A mixture of polyvinyl alcohol (the mass ratio of polylactic acid and polyvinyl alcohol is 1:1); the cell growth factors are VEGF, TGF-β and b-FGF, and the mass fractions in material solution A are 0.002% and 0.002% respectively and 0.001%;

[0055] (2) adding nano-hydroxyapatite (n-HA) to the mixed solution of type I collagen / polylactic acid / polyvinyl alcohol prepared in step (1), and adding cell growth factors to obtain material solution B, wherein , n-HA by CaCl 2 and Na 3 PO 4 According to the calcium / phosphorus molar ratio 1.67:1, the reaction synthesis under the condition of pH is 12, and the mass fraction in the material solution B is 40%; the cell growth factor is a mixture of BMP-7 and PDGF, in the material solution B The mass fractions are 0...

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Abstract

The invention relates to a bionic artificial bone scaffold and a preparation method thereof. The method comprises the following steps: preparing material solutions A and B; utilizing an electrostatic spinning method to respectively prepare the material solutions A and B into a central channel layer and a peripheral column layer of a scaffold, wherein nanometer fibers in the central channel layer are in same spiral directions and the fibers in two adjacent nanometer fiber layers in the peripheral column layer are in opposite spiral directions; micro-level finely simulating a three-dimensional microenvironment of an osteon; hierarchically adding growth factors, supplying a differential inducing environment to seed cells BMSCs and constructing the bionic artificial bone scaffold with vascularizing tissues in the central channel layer and bone tissues in the peripheral column layer. The staggered orientation of the nanometer fibers in the scaffold can greatly enhance the structural stability; effective mechanic support is supplied to a bone defect part; the mutually connected network frame thereof is beneficial to cell permeation growth and blood vessel and nerve growth and also is convenient to transfer nutrient substances and metabolic wastes; the bionic artificial bone scaffold has ideal popularization and application values in the fields of biomedical materials and tissue engineering.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and in particular relates to a bionic artificial bone support and a preparation method thereof. Background technique [0002] There are tens of millions of patients with bone defects every year in the world, making bone repair scaffold materials one of the most clinically demanded biomedical materials. For a long time, the research and development of ideal artificial bone scaffolds to treat bone defects has been a hot research topic for scientists. [0003] There are different types of artificial bone repair materials currently used clinically, made of metals, ceramics or polymers, which have their own advantages and disadvantages in terms of biological activity, biocompatibility, biodegradability, mechanical properties and service life. . However, the common problem with the existing artificial bone scaffolds is that although the interface between the implant and the bone can form a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/12A61L27/20A61L27/16A61L27/18A61L27/22A61L27/58A61L27/54D04H1/728D04H1/4382D01D5/00
CPCA61L27/12A61L27/16A61L27/18A61L27/20A61L27/22A61L27/24A61L27/54A61L27/58A61L2430/02D01D5/0061D01D5/0084D04H1/4382D04H1/728C08L5/08C08L5/04C08L29/04C08L71/02C08L67/04
Inventor 江虹龙平李梦伟孙菊江何雨薇董世武李振声丁海滨
Owner ARMY MEDICAL UNIV
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