The invention discloses a Zn-Mn series zinc alloy and a preparing method and application thereof. The Zn-Mn series zinc alloy comprises Zn and Mn, wherein by weight percent, the mass percent of Mn in the zinc alloy ranges from 0% to 30%, however the mass percent of the Mn cannot be 0. The preparing method comprises following steps: firstly, Zn and Mn are mixed, and a mixture is obtained; secondly, the mixture is treated according to the step of a or b, cooling is carried out, and the zinc alloy is obtained, wherein under the CO2 and SF6 atmosphere protection, the mixture is smelted or sintered, and under the vacuum atmosphere protection, hydrogen is dissolved in the mixture to be smelted. The prepared Zn-Mn series zinc alloy is excellent in mechanical property, long-term effective mechanical support is provided in the machine body, the excellent cellular biocompatibility, blood compatibility and tissue and organ compatibility can be achieved, and the alloy can be used for preparing of biomedical implant materials.