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Culture method for induced differentiation of hair follicle stem cells into vascular endothelial cells

A hair follicle stem cell and vascular endothelium technology, applied in vascular endothelial cells, cell dissociation methods, cell culture active agents, etc., can solve the problems of easy aging of vascular endothelial cells, stenosis of bridge blood vessels, limited sources of materials, etc., and reduce immune rejection. Equal probability of reacting and spreading disease, promoting repair, rich source effect

Inactive Publication Date: 2016-07-13
HANGZHOU CITY XIAOSHAN DISTRICT TRADITIONAL CHINESE MEDICAL HOSPITAL
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Problems solved by technology

However, the source of autologous ECs is limited, and it is easy to cause stenosis and occlusion of the grafted blood vessels after harvesting, and it is easy to aggravate the damage to the patient. Moreover, the vascular endothelial cells that have been isolated and cultured in the primary generation are very easy to age, have a short cell cycle, and have very limited proliferation ability.
These all limit the direct acquisition and application of ECs

Method used

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  • Culture method for induced differentiation of hair follicle stem cells into vascular endothelial cells
  • Culture method for induced differentiation of hair follicle stem cells into vascular endothelial cells
  • Culture method for induced differentiation of hair follicle stem cells into vascular endothelial cells

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Embodiment Construction

[0033] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0034] 1. Isolation, culture, purification and identification of rat hair follicle stem cells

[0035] 1.1 Isolation of rat hair follicle stem cells

[0036]Newborn 1-week-old SD rats were killed by cervical dislocation, put into a beaker filled with 75% ethanol for disinfection, and then taken out. In an ultra-clean bench, use ophthalmic scissors to shorten the tentacles and cut off the skin of the tentacles, rinse once with 75% ethanol, and then rinse three times with PBS. Then digest with 1% collagenase type IV and 1% Dispase enzyme mixture at 37°C for 90min, and wash twice with PBS. Under a stereomicroscope, use a 1ml syringe needle to desheath the hair follicle, cut off both ends, leaving the bulge, inoculate the bulge of the hair follicle into a pre-coated culture dish, and add 1ml of complete medium.

[0037] The components ...

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Abstract

The invention relates to the field of stem cell induction culture, in particular to a culture method for induced differentiation of hair follicle stem cells into vascular endothelial cells. The culture method for induced differentiation of the hair follicle stem cells into the vascular endothelial cells comprises steps as follows: (1) separation of rat hair follicle stem cells; (2) culture of the rat hair follicle stem cells; (3) purification of the rat hair follicle stem cells; (4) culture of in-vitro induced differentiation of the rat hair follicle stem cells into the vascular endothelial cells. The vascular endothelial cells for promoting early vascularization of skin after trauma are obtained with the culture method, so that repair of traumatic skin is promoted; meanwhile, seed cell sources can be provided for solving problems of vascularization of tissue engineering skin, ischemic disease treatment through cell transplantation and the like.

Description

technical field [0001] The invention relates to the field of stem cell induction culture, in particular to a culture method for inducing hair follicle stem cells to differentiate into vascular endothelial cells. Background technique [0002] Stem cells are the first element of regenerative medicine, and in-depth research on their biological characteristics can guide the development of regenerative medicine. Stem cells commonly used in regenerative medicine are divided into embryonic stem cells and adult stem cells. Due to the limited sources of embryonic stem cells and serious ethical concerns Ethically controversial, so adult stem cells seem more suitable for application in regenerative medicine. [0003] Studies have shown that hair follicle stem cells (Hair Follicle Stem Cells, HFSCs) are a type of stem cells that exist in the protuberance of the outer root sheath of hair follicles, and have the characteristics of undifferentiation, self-renewal and strong proliferation i...

Claims

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Application Information

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IPC IPC(8): C12N5/071C12N5/074
CPCC12N5/069C12N2500/32C12N2500/35C12N2500/84C12N2500/90C12N2501/11C12N2501/115C12N2501/165C12N2501/30C12N2506/03C12N2509/00
Inventor 全仁夫杜伟斌郑宣李强曹国平
Owner HANGZHOU CITY XIAOSHAN DISTRICT TRADITIONAL CHINESE MEDICAL HOSPITAL
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