Injection, preparation method of injection and application of injection in dental pulp regeneration

A technology of injection and endodontics, applied in the field of biomaterials and clinical medicine

Pending Publication Date: 2021-01-15
PEKING UNIV SCHOOL OF STOMATOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, studies have found that the new tissue in the root canal is a mixture of cement...

Method used

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  • Injection, preparation method of injection and application of injection in dental pulp regeneration
  • Injection, preparation method of injection and application of injection in dental pulp regeneration
  • Injection, preparation method of injection and application of injection in dental pulp regeneration

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preparation example Construction

[0032] The embodiments of the present disclosure provide an injection, a preparation method of the injection, and an application of the injection in pulp regeneration. Among them, an injection used in dental pulp regeneration includes: a composite preparation as a dispersed phase; and a dispersion liquid as a continuous phase.

[0033] In the embodiment of the present disclosure, the composite preparation includes medicine, microcarrier and stem cells, the medicine is encapsulated inside the microcarrier, and the stem cells are attached to the surface of the microcarrier.

[0034] Among them, the drug is a protein growth factor or a small molecule drug, which is used to achieve stem cell expansion, odontogenic differentiation and angiogenesis differentiation, and promote regeneration of vascularized and neuralized dental pulp-like tissues. The proteinaceous growth factor is selected from the group consisting of stromal cell-derived factor (SDF), amelogenin, vascular endothelia...

Embodiment 1

[0052] Embodiment 1: Preparation of injectable simvastatin sustained-release GelMA crystal gel microsphere microcarrier

[0053] (1) Dissolve 1 gram of GelMA in 20 milliliters of deionized water, add 0.02 gram of photoinitiator I2959 and 50 milligrams of water-soluble surfactant Tween 60 and mix well to obtain solution A (water phase); add 500 milligrams to 200 milliliters of oil Milligrams of oil-soluble surfactant Span 80, mixed uniformly to obtain solution B (outer oil phase). Simvastatin (SIM) (0.1 mg, 1 mg, and 10 mg), 100 mg of aliphatic polyester PLGA, and 50 mg of oil-soluble surfactant Span 80 were dissolved in 2 mL of dichloromethane solution, respectively, to obtain drug solution C ( inner oil phase).

[0054] (2) Add solution C to solution A, in an ice-water bath environment, and sonicate for 3 minutes with a power of 200 W to obtain PLGA (SIM) / GelMA oil-in-water primary emulsion D.

[0055] (3) Add the oil-in-water primary emulsion D into the solution B, in an i...

Embodiment 2

[0058] Example 2: Preparation of Injectable Growth Factor Slow Release GelMA Crystal Colloid Microsphere Microcarrier

[0059] (1) Dissolve 1 gram of GelMA in 20 milliliters of deionized water, add 0.02 gram of photoinitiator L2959, 50 milligrams of water-soluble surfactant Tween 60 and 10 micrograms of VEGF and mix uniformly to obtain solution A (water phase); in 200 milliliters Add 500 mg of oil-soluble surfactant Span 80 to the oil, and mix well to obtain solution B (oil phase).

[0060] (2) Add solution A to solution B, in an ice-water bath environment, and mechanically stir at 300 rpm for 10 minutes to obtain a water-in-oil emulsion of GelMA / oil.

[0061] (3) Put the water-in-oil emulsion obtained in step (2) into -20°C for 30 minutes, -80°C for 30 minutes, and liquid nitrogen for 10 minutes to cool down in sequence, and cross-link with 365nm ultraviolet light to obtain growth factor-loaded GelMA microspheres.

[0062] (4) The growth factor-loaded GelMA crystal gel micro...

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Abstract

The invention relates to the technical field of biological materials and clinical medicine, and provides an injection, a preparation method of the injection and application of the injection in dentalpulp regeneration. The injection comprises a composite preparation as a dispersion phase and a dispersion liquid as a continuous phase. The composite preparation comprises a drug, a microcarrier and stem cells, the drug is wrapped in the microcarrier, and the stem cells are attached to the surface of the microcarrier. The dispersion liquid is selected from at least one of a group consisting of a balanced salt solution, a serum-free culture medium and injectable hydrogel. The injection is mainly applied to regeneration of clinical functional dental pulp tissues, and regeneration of dental pulpin affected root canals is realized. By utilizing the injection, the formation of the nerve and vascularized dental pulp-like tissue in the root canal can be realized, the nutrition, defense and sensory functions of the dental pulp tissue are recovered, and the service life of the affected tooth is prolonged.

Description

technical field [0001] The disclosure relates to the technical field of biomaterials and clinical medicine, and specifically relates to an injection used in pulp regeneration, a preparation method of the injection, and an application of the injection in pulp regeneration, so as to realize the restoration of the dental pulp in the root canal of the affected tooth. regeneration. Background technique [0002] When a tooth suffers from dental caries, periodontal disease or trauma, irreversible inflammation or necrosis may occur in the dental pulp. Clinically, for young permanent teeth with pulp necrosis or apical periodontitis, apex induction or apical barrier surgery are mainly used. Permanent tooth pulp necrosis uses root canal therapy to remove necrotic infected tissue in the root canal, and uses drugs to fill and seal the root canal to eliminate inflammation. However, root apex induction, root apex barrier surgery and root canal treatment cannot achieve the formation of fu...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/22A61L27/38A61L27/50A61L27/54
CPCA61L27/3834A61L27/3865A61L27/222A61L27/18A61L27/54A61L27/50A61L2430/12A61L2400/06A61L2300/414A61L2300/216A61L2300/412
Inventor 葛立宏赵玉鸣袁晓静王媛媛汪晓彤韩建民袁作楹熊春阳
Owner PEKING UNIV SCHOOL OF STOMATOLOGY
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