Vascularized tumor microfluidic organ chip used for culture in vitro, and preparation method for vascularized tumor microfluidic organ chip

Abstract

The invention provides a vascularized tumor microfluidic organ chip used for culture in vitro, and a preparation method for the vascularized tumor microfluidic organ chip. The chip comprises a PMMA module and a glass sheet, wherein the PMMA module is provided with three through holes which penetrate through the thickness direction of the PMMA module; the periphery of the upper surface of the glasssheet and the periphery of the lower surface of the PMMA module are bonded into a whole through a bonding layer, and the three through holes independently form a first cavity used for containing a tumor sphere, a second cavity used for storing culture solution, and a third cavity; in addition, the middle area of the lower surface of the PMMA module and the glass sheet form a hollow tissue cavity;and a static pressure difference generated by the culture solution with a liquid level height difference can accelerate a three-dimensional blood capillary network to be generated, and through co-culture of the three-dimensional blood capillary network and the tumor sphere, blood capillaries can be collected and can be constructed into three-dimensional vascularized tumor micro-tissues. The chipovercomes the defects of time consumption and high cost when the microfluidic organ chip is manufactured at present, the chip has a simple technology, and an application value is provided especially for researching in vitro vascularized tumor diseases.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering, in particular, to a vascularized tumor microfluidic organ chip for in vitro culture and a preparation method thereof. Background technique [0002] As a facilitator of material exchange between local tissues and the systemic circulation, the vascular system is important in the transport of nutrients, oxygen, and waste in the human body. Due to its own functionality and complexity, with the development of tissue engineering, the study of the vascular system has far-reaching applications in the therapeutic engineering of various vascular-related diseases, and also provides a basis for potential drug screening methods. [0003] At present, cancer has become one of the biggest challenges faced by the world's health field. The efficacy of drugs evaluated by traditional animal models often cannot predict their effects on the human body. Therefore, the development of new platforms for the...

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Problems solved by technology

[0009] Due to the time-consuming manufacturing process of traditional PDMS chips, and the fact that PDMS materials themselves easily absorb small molecules and affect drug screening, J.Ko, J.Ahn et al. wrote "Tumor spheroid-on-a -chip:standardized microfluidic culture platform for investigating tumorangiogenesis", constructing a 3D printing polystyrene (PS) material method based on injection molding method, constructing an open vascularized tumor platform that can be combined with a 96-well plate, and solving the problem of PDMS absorbing small molecules Each structural unit of the platform has two culture fluid reservoirs and a circular through hole for placing 3D tumor spheres, which can achieve precise tumor positioning and tumor-induced angiogenesis, but the entire platform is limited High print production costs

[0010] In summary, the currently reported fabrication of in vitro vascularized tumor microfluidic platforms has not achieved an ideal compromise between time and cost. Both traditional techniques and emerging 3D printing technologies have their own shortcomings. There is an urgent need to realize personalized in vitro vascularized chip structures

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