Method for preparing multilayer spiral urethral tissue engineering scaffold

A tissue engineering stent and urethra technology, which is applied in the field of preparation of tissue engineering urethral stents for urethral diseases, can solve the problems of hindering the physiological activity of the urethra, sudden disintegration, slow expansion rate, etc., and achieves the effect of reducing surface coverage and avoiding urethral blockage.

Active Publication Date: 2020-01-14
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The defect of the existing urethral stent preparation method is that although the stent formed by curling the polymer film is easy to manufacture and has high compliance, it is prone to sudden disintegration during the degradation process, forming larger fragments, which is easy to cause The urethra is blocked, which hinders the normal physiological activities of the urethra; in addition, relying solely on the self-expandability of the spiral stent, the expansion rate is relatively slow, and there is a possibility of dislocation after stent implantation
As a result, the printed product is basically in a glass state at room temperature, with low strength and high brittleness, which cannot meet the mechanical performance requirements of the bracket.
[0006] Simply implanting the carrying cells into the vicinity of the defect in the organism is prone to the problem of cell death due to insufficient supply of nutrients

Method used

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  • Method for preparing multilayer spiral urethral tissue engineering scaffold
  • Method for preparing multilayer spiral urethral tissue engineering scaffold
  • Method for preparing multilayer spiral urethral tissue engineering scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A kind of preparation of urethra tissue engineering support, comprises the steps:

[0044] (1) Using polylactic acid-glycolic acid (PLGA) copolymer as raw material (LA:GA=75:25, Mv=100000), after three-dimensional modeling, import the .stl file into the fused deposition 3D printer and print out two surfaces A rectangular long embryo body with a coverage rate of 20% and a spline curve pattern; the wall thickness of the support, that is, the thickness of the rectangular long embryo body is 0.1 mm; 1 3.0mm and φ 2 On a 5.6mm mold; both ends are fixed with thin copper wires; then placed in a vacuum oven at 120 ° C for 10 minutes to promote polymer crystallization and the shape of the bracket; after cooling, take out the mold to obtain monolayers with different diameters The bracket is then cut into a long tube with a length of 30.0mm.

[0045] (2) The stents of equal length and matching diameter are coaxially nested and fixed to form a double-layer stent, and one end of t...

Embodiment 2

[0054] A kind of preparation of urethra tissue engineering support, comprises the steps:

[0055] (1) Using polylactic acid (molecular weight: 100,000) as the raw material, import the .stl file into the fused deposition 3D printer after 3D modeling and print out two rectangular strips with a surface coverage of 30% and spline curve patterns Body, the wall thickness of the stent, that is, the thickness of the rectangular long strip embryo body is 0.3mm; respectively, two rectangular long strip embryo bodies are helically crimped with diameters of φ 1 = 4.0mm and φ 2 = 6.8mm on the mold; both ends are respectively fixed with thin copper wires; then placed in a vacuum oven at 90°C for 10 minutes to be annealed and shaped; after the mold is cooled, the mold is taken out to obtain single-layer stents of different diameters, which are all cut to length A long tube of 40.0mm.

[0056] (2) A double-layered stent is formed by coaxially nesting and fixing stents of equal length and ma...

Embodiment 3

[0065] A kind of preparation of urethra tissue engineering support, comprises the steps:

[0066] (1) Using polycaprolactone (molecular weight: 50,000) as the raw material, import the .st1 file into the fused deposition 3D printer after three-dimensional modeling and print out two rectangles with a surface coverage of 40% and spline curve patterns. Strip-shaped embryo body, the wall thickness of the support, that is, the thickness of the rectangular long strip embryo body is 0.4mm; two rectangular long strip embryo bodies are helically crimped on diameters of φ 1 = 4.5mm and φ 2 = 7.3mm on the mold; both ends are respectively fixed with thin copper wires; then placed in a vacuum oven at 30°C for annealing for 20 minutes and finalized; after the mold is cooled, take out the mold to obtain single-layer stents with different diameters, and cut them into lengths A long tube of 60.0mm.

[0067](2) The stents of equal length and matching diameter are coaxially nested and fixed to ...

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Abstract

The invention discloses a method for preparing a multilayer spiral urethral tissue engineering scaffold. A thermoplastic degradable polymer is used as a printing raw material, a rectangular strip embryoid body with hollow patterns by adopting a fused deposition type 3D printer, an embryoid body spiral tube is obtained by spirally curling, and after heat treatment and nesting, a double-layer scaffold consisting of an inner-layer embryoid body spiral tube and an outer-layer embryoid body spiral tube is obtained, one end of the double-layer scaffold is sewed by a polylactic acid suture, an endothelial cell sheet layer and a urothelial cell sheet layer which are transfected with vascular endothelial growth factors and fibroblast growth factors during culture and a smooth muscle cell sheet layer which is transfected with human platelet growth factors during culture are lined on the inner wall of the outer embryoid body spiral tube in sequence, extracellular matrix is filled, the other end of the double-layer scaffold is sewed, and the urethral tissue engineering scaffold can be obtained after thrombin is fixed. The scaffold prepared by the invention can keep a urethra smooth, is beneficial to healing the injured urethra tissue, realizes the vascularization of a urethra scaffold in time and can quickly realize the revascularization.

Description

technical field [0001] The invention belongs to the field of biomedical devices, and in particular relates to a preparation technology for a tissue-engineered urethral stent for treating urethral diseases such as urethral defects. [0002] Background of the invention [0003] The defect of the existing urethral stent preparation method is that although the stent formed by curling the polymer film is easy to manufacture and has high compliance, it is prone to sudden disintegration during the degradation process, forming larger fragments, which is easy to cause Urethral blockage hinders normal physiological activities of the urethra; in addition, relying solely on the self-expandability of the helical stent, the expansion rate is relatively slow, and there is a possibility of dislocation after stent implantation. The preparation process of the braided stent is slow, inefficient, time-consuming and lacks precise control of the stent structure; the compliance is insufficient and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/12A61L31/14A61L31/16A61L31/00B29C64/118B33Y80/00
CPCA61L31/005A61L31/129A61L31/14A61L31/148A61L31/16A61L2300/30A61L2300/604B33Y80/00B29C64/118
Inventor 白晶王先丽张磊薛烽程兆俊范启洲
Owner SOUTHEAST UNIV
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