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Method for preparing retinal pigment epithelia

A technology for retinal pigment and epithelial cells, applied in the field of preparation of retinal pigment epithelial cells, can solve the problems of increased animal-derived pollution, long differentiation time, low differentiation efficiency, etc., to shorten the differentiation time, avoid potential risks, and improve the differentiation efficiency. Effect

Inactive Publication Date: 2012-08-01
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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Problems solved by technology

The disadvantage of this method is that, first, it has high requirements for embryonic stem cells, and only some cell lines can differentiate enough RPE cells; second, it takes a long time for differentiation, usually 4-8 weeks to form pigmented foci; Third, the feeder cells themselves have the effect of inhibiting the differentiation of stem cells, making the differentiation efficiency low, usually 10-20%; Possibility of sexual contamination, it is also reported to use human-derived feeder layer, but its technical difficulty is relatively high, and the source is limited, so it is difficult to put it into practice

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  • Method for preparing retinal pigment epithelia
  • Method for preparing retinal pigment epithelia
  • Method for preparing retinal pigment epithelia

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Embodiment Construction

[0021] The present invention will be described in detail below in conjunction with the embodiments and drawings, which are only for illustration and not limiting the scope of application of the present invention. The present invention is not limited to the following embodiments or examples, and any modifications and variations that do not violate the spirit of the present invention shall be included within the scope of the present invention.

[0022] The method for preparing retinal pigment epithelial cells in this embodiment uses a liver X receptor (LXR) activator to induce stem cells to differentiate into retinal pigment epithelial cells.

[0023] In this embodiment, the stem cells are human embryonic stem cells. Of course, other types of human stem cells or stem cells of other species can also be used, all of which can achieve the purpose of the invention, but the effect of using human embryonic stem cells is better.

[0024] In this embodiment, the induction of differentia...

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Abstract

The invention discloses a method for preparing retinal pigment epithelia. According to the method, stem cells are induced by utilizing a liver X receptor (LXR) activating agent to be differentiated into the retinal pigment epithelia. Compared with the prior art, the method for inducing the stem cells to be differentiated into the retinal pigment epithelia by utilizing the LXR receptor activating agent has the advantages that: a feed layer is not used, so that potential risks caused by an animal source feed layer are avoided; and the differentiation efficiency can be improved, and the differentiation time can be shortened.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preparing retinal pigment epithelial cells. Background technique [0002] Retinal degenerative diseases are a class of serious blinding diseases, mainly including Retinal pigment degeneration (RP for short) and age-related macular degeneration (Agerelated macular degeneration, AMD for short). The pathogenesis of such diseases is unknown, and the effective prevention and treatment methods are limited, which seriously endangers the quality of life of patients and causes hundreds of billions of huge losses worldwide every year. The pathological process of these diseases is caused by the degeneration and death of retinal pigment epithelium (RPE). Therefore, the use of RPE cells for replacement transplantation therapy has become an important means for the treatment of such diseases, but the source of RPE cells is limited, which limits the clinical application of transplantat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
Inventor 阴正勤曾玉晓徐海伟段平刘俊
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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